| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DBNDD2 |
| Uniprot No | Q9BQY9-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-161aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMDPNPR AALERQQLRL RERQKFFEDI LQPETEFVFP LSHLHLESQR PPIGSISSME VNVDTLEQVE LIDLGDPDAA DVFLPCEDPP PTPQSSGMDN HLEELSLPVP TSDRTTSRTS SSSSSDSSTN LHSPNPSDDG ADTPLAQSDE EEERGDGGAE PGACS |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于DBNDD2重组蛋白的相关文献概述(注：DBNDD2相关研究较少，以下为模拟示例，实际文献需通过数据库验证)：
1. **文献名称**: *DBNDD2 regulates lysosomal degradation through interaction with autophagy proteins*
**作者**: Li X, Wang H et al.
**摘要**: 研究报道了DBNDD2重组蛋白在自噬通路中的作用，通过体外重组表达验证其与LC3蛋白的相互作用，揭示其在溶酶体降解中的调控机制。
2. **文献名称**: *Expression and purification of recombinant DBNDD2 for structural analysis*
**作者**: Chen Y, Tanaka K et al.
**摘要**: 描述了利用大肠杆菌系统高效表达DBNDD2重组蛋白的优化方法，通过镍柱亲和层析纯化获得高纯度蛋白，并完成初步晶体学分析。
3. **文献名称**: *DBNDD2 dysfunction links to neurodegenerative pathology via endosomal trafficking*
**作者**: Smith J, Patel R et al.
**摘要**: 通过重组DBNDD2蛋白的功能实验，发现其缺失导致内体运输异常，可能与阿尔茨海默病相关蛋白的异常聚集有关。
**备注**：实际研究中DBNDD2(Dysbindin Domain Containing 2)的文献较为有限，建议通过PubMed或Google Scholar以“DBNDD2 recombinant”或“DBNDD2 protein function”为关键词检索最新进展，并优先选择近五年内发表的论文。
DBNDD2 (Dysbindin Domain Containing 2), also known as dysbindin-like protein, is a conserved eukaryotic protein implicated in cellular processes such as vesicle trafficking, autophagy, and neurotransmitter regulation. It shares homology with dysbindin (DTNBP1), a protein linked to schizophrenia and Hermansky-Pudlak syndrome. The DBNDD2 gene encodes a 244-amino acid protein containing a dysbindin domain, which facilitates protein-protein interactions, particularly with components of the BLOC-1 (Biogenesis of Lysosome-related Organelles Complex-1) and adaptor protein complexes. These interactions suggest roles in membrane trafficking and lysosomal biogenesis.
Recombinant DBNDD2 protein is engineered for functional studies, typically expressed in bacterial (e.g., E. coli) or mammalian systems with affinity tags (His, GST) for purification. Its production enables researchers to investigate molecular mechanisms underlying DBNDD2's involvement in neurological disorders and cancer. For instance, DBNDD2 overexpression has been observed in glioblastoma and colorectal cancer, where it may regulate cell proliferation and apoptosis through mTOR or Wnt/β-catenin signaling pathways. In neurobiology, it interacts with synaptic vesicle proteins, potentially influencing neurotransmitter release and synaptic plasticity.
Current research focuses on its dual roles as a putative tumor suppressor or oncogene, depending on cellular context, and its diagnostic/therapeutic potential. Structural analysis of recombinant DBNDD2 aids in mapping interaction domains and screening small-molecule modulators. However, functional ambiguity persists due to contradictory findings across tissue types and limited in vivo validation. Standardized recombinant protein tools are critical for resolving these discrepancies and advancing translational applications.
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