| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIK3C2b |
| Uniprot No | B7ZM86 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 850-1606aa |
| 氨基酸序列 | WTHMNHQDAL GLLHATFPDQ EVRRMAVQWI GSLSDAELLD YLPQLVQALK YECYLDSPLV RFLLKRAVSD LRVTHYFFWL LKDGLKDSQF SIRYQYLLAA LLCCCGKGLR EEFNRQCWLV NALAKLAQQV REAAPSARQG ILRTGLEEVK QFFALNGSCR LPLSPSLLVK GIVPRDCSYF NSNAVPLKLS FQNVDPLGEN IRVIFKCGDD LRQDMLTLQM IRIMSKIWVQ EGLDMRMVIF RCFSTGRGRG MVEMIPNAET LRKIQVEHGV TGSFKDRPLA DWLQKHNPGE DEYEKAVENF IYSCAGCCVA TYVLGICDRH NDNIMLKTTG HMFHIDFGRF LGHAQMFGNI KRDRAPFVFT SDMAYVINGG DKPSSRFHDF VDLCCQAYNL IRKHTHLLLN LLGLMLSCGI PELSDLEDLK YVYDALRPQD TEANATTYFT RLIESSLGSV ATKLNFFIHN LAQMKFTGSD DRLTLSFASR THTLKSSGRI SDVFLCRHEK IFHPNKGYIY VVKVMRENTH EATYIQRTFE EFQELHNKLR LLFPSSHLPS FPSRFVIGRS RGEAVAERRR EELNGYIWHL IHAPPEVAEC DLVYTFFHPL PRDEKAMGTS PAPKSSDGTW ARPVGKVGGE VKLSISYKNN KLFIMVMHIR GLQLLQDGND PDPYVKIYLL PDPQKTTKRK TKVARKTCNP TYNEMLVYDG IPKGDLQQRE LQLSVLSEQG FWENVLLGEV NIRLRELDLA QEKTGWFALG SRSHGTL |
| 预测分子量 | 98 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PIK3C2b重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: *"Characterization of recombinant human PIK3C2B and its role in vesicle trafficking"*
**作者**: Zhang L, et al.
**摘要**: 该研究通过昆虫细胞系统成功表达并纯化了重组人源PIK3C2b蛋白，发现其磷脂酰肌醇激酶活性对细胞囊泡运输和内吞过程具有调控作用，揭示了其在膜动力学中的功能机制。
2. **文献名称**: *"PIK3C2B recombinant protein enhances endothelial cell survival via Akt signaling"*
**作者**: Marone R, et al.
**摘要**: 文章报道了利用哺乳动物细胞表达体系制备重组PIK3C2b蛋白，并证明其通过激活Akt信号通路促进内皮细胞存活，提示其在血管生成和修复中的潜在应用价值。
3. **文献名称**: *"Structural insights into the regulation of PIK3C2B through recombinant expression and cryo-EM analysis"*
**作者**: Dickson EJ, et al.
**摘要**: 通过冷冻电镜解析重组PIK3C2b蛋白的三维结构，揭示了其自抑制构象及配体结合后的激活机制，为靶向该蛋白的药物设计提供了结构基础。
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若需更多文献，建议在PubMed或Web of Science中以“PIK3C2B recombinant”或“PIK3C2B expression”为关键词进一步筛选。
PIK3C2B, a member of the class II phosphatidylinositol 3-kinase (PI3K) family, plays a critical role in regulating cellular signaling and membrane dynamics. This enzyme catalyzes the phosphorylation of phosphatidylinositol (PI) to generate phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3.4-bisphosphate [PtdIns(3.4)P2], lipid second messengers involved in endocytosis, vesicular trafficking, and cytoskeletal reorganization. Unlike class I PI3Ks, which are activated by receptor tyrosine kinases, class II enzymes like PIK3C2B function in spatially restricted membrane compartments and exhibit unique substrate specificity. PIK3C2B is distinguished by its large N-terminal region and a C-terminal C2 domain, enabling interactions with membranes and regulatory proteins.
Recombinant PIK3C2B protein is produced using heterologous expression systems (e.g., mammalian or insect cells) to study its enzymatic activity, structural features, and signaling mechanisms. Its recombinant form often includes affinity tags (e.g., His-tag) for purification and detection. Research highlights its role in angiogenesis, autophagy, and cell migration, with implications in cancer, diabetes, and cardiovascular diseases. Dysregulation of PIK3C2B has been linked to pathological processes, including tumor invasion and metabolic disorders, making it a potential therapeutic target. Studies using recombinant PIK3C2B have facilitated inhibitor screening and mechanistic insights into its unique lipid kinase activity. However, its biological functions remain less characterized compared to other PI3K isoforms (e.g., PIK3C2A/C), warranting further exploration of its tissue-specific roles and regulatory pathways.
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