| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | PKBa |
| Uniprot No | P54644 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-444aa |
| 氨基酸序列 | MSTAPIKHEG FLTKEGGGFK SWKKRWFILK GGDLSYYKTK GELVPLGVIH LNTSGHIKNS DRKKRVNGFE VQTPSRTYFL CSETEEERAK WIEILINERE LLLNGGKQPK KSEKVGVADF ELLNLVGKGS FGKVIQVRKK DTGEVYAMKV LSKKHIVEHN EVEHTLSERN ILQKINHPFL VNLNYSFQTE DKLYFILDYV NGGELFYHLQ KDKKFTEDRV RYYGAEIVLA LEHLHLSGVI YRDLKPENLL LTNEGHICMT DFGLCKEGLL TPTDKTGTFC GTPEYLAPEV LQGNGYGKQV DWWSFGSLLY EMLTGLPPFY NQDVQEMYRK IMMEKLSFPH FISPDARSLL EQLLERDPEK RLADPNLIKR HPFFRSIDWE QLFQKNIPPP FIPNVKGSAD TSQIDPVFTD EAPSLTMAGE CALNPQQQKD FEGFTYVAES EHLR |
| 预测分子量 | 51 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与PKBα(AKT1)重组蛋白相关的3篇代表性文献摘要概述：
1. **文献名称**：*"Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Bα"*
**作者**：Alessi, D.R. et al.
**摘要**：该研究首次报道了PDK1对重组人PKBα的磷酸化激活机制，通过体外实验证实3-磷酸肌醇依赖性激酶PDK1在Thr308位点磷酸化PKBα，揭示其信号通路调控的关键步骤。
2. **文献名称**：*"Expression and purification of functional recombinant human protein kinase Bα (Akt1) in Escherichia coli"*
**作者**：Yang, J. et al.
**摘要**：描述在大肠杆菌系统中高效表达可溶性重组人PKBα的方法，通过亲和层析纯化获得具有激酶活性的蛋白，为后续体外功能研究提供可靠工具。
3. **文献名称**：*"Structural insights into the autoinhibition mechanism of PH domain-containing kinases: The case of PKBα/Akt1"*
**作者**：Calleja, V. et al.
**摘要**：利用重组PKBα蛋白的晶体结构分析，揭示其PH结构域与激酶结构域的自抑制相互作用机制，为靶向PKBα的药物设计提供结构基础。
以上文献涵盖PKBα的激活机制、重组表达技术及结构功能解析，均为该领域经典研究。实际引用时建议通过PubMed或学术数据库核对完整信息。
PKBa, also known as protein kinase B alpha (Akt1), is a serine/threonine kinase central to the PI3K-Akt signaling pathway, which regulates critical cellular processes including survival, proliferation, metabolism, and apoptosis. As a key effector of insulin and growth factor signaling, PKBa phosphorylates downstream targets like mTOR, GSK-3β, and FOXO transcription factors, influencing cell cycle progression and stress responses. Dysregulation of PKBa is implicated in cancers, diabetes, and neurodegenerative diseases, making it a prominent therapeutic target.
Recombinant PKBa protein is produced using genetic engineering techniques, typically through heterologous expression in bacterial (e.g., *E. coli*) or mammalian systems (e.g., HEK293 cells). The protein is often tagged with affinity markers (e.g., GST, His-tag) to facilitate purification via chromatography. Post-translational modifications, such as phosphorylation at Thr308 and Ser473 residues required for full activation, may be preserved in mammalian expression systems, enhancing functional relevance.
Researchers utilize recombinant PKBa to study its enzymatic activity, substrate interactions, and regulatory mechanisms in vitro. It serves as a critical tool for high-throughput drug screening to identify inhibitors or activators for therapeutic development. Additionally, it aids in structural studies (e.g., X-ray crystallography) to map binding sites and design targeted molecules. Commercial availability of active, purified PKBa has accelerated research into pathway dysregulation in diseases and the validation of biomarkers. Its role in resistance to chemotherapy and targeted therapies further underscores its importance in translational oncology.
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