| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DVL3 |
| Uniprot No | Q92997 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-716aa |
| 氨基酸序列 | MGETKIIYHLDGQETPYLVKLPLPAERVTLADFKGVLQRPSYKFFFKSMDDDFGVVKEEISDDNAKLPCFNGRVVSWLVSAEGSHPDPAPFCADNPSELPPPMERTGGIGDSRPPSFHPHAGGGSQENLDNDTETDSLVSAQRERPRRRDGPEHATRLNGTAKGERRREPGGYDSSSTLMSSELETTSFFDSDEDDSTSRFSSSTEQSSASRLMRRHKRRRRKQKVSRIERSSSFSSITDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNEINFENMSNDDAVRVLREIVHKPGPITLTVAKCWDPSPRGCFTLPRSEPIRPIDPAAWVSHTAAMTGTFPAYGMSPSLSTITSTSSSITSSIPDTERLDDFHLSIHSDMAAIVKAMASPESGLEVRDRMWLKITIPNAFIGSDVVDWLYHNVEGFTDRREARKYASNLLKAGFIRHTVNKITFSEQCYYIFGDLCGNMANLSLHDHDGSSGASDQDTLAPLPHPGAAPWPMAFPYQYPPPPHPYNPHPGFPELGYSYGGGSASSQHSEGSRSSGSNRSGSDRRKEKDPKAGDSKSGGSGSESDHTTRSSLRGPRERAPSERSGPAASEHSHRSHHSLASSLRSHHTHPSYGPPGVPPLYGPPMLMMPPPPAAMGPPGAPPGRDLASVPPELTASRQSFRMAMGNPSEFFVDVM |
| 预测分子量 | 85.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DVL3重组蛋白的3篇代表性文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库查询确认)：
---
1. **文献名称**：*Structural and functional analysis of recombinant DVL3 in Wnt/β-catenin signaling*
**作者**：Li, Y., Zhang, W., & Chen, X.
**摘要**：本研究通过大肠杆菌系统成功表达并纯化了重组DVL3蛋白，解析了其N端结构域的晶体结构，揭示了其与Frizzled受体相互作用的分子机制，并证明其在体外可增强Wnt/β-catenin通路的信号传导。
2. **文献名称**：*Expression optimization and purification of human DVL3 protein for biochemical assays*
**作者**：Smith, J.R., Kumar, S., & Patel, R.
**摘要**：作者优化了人源DVL3重组蛋白在原核和真核系统中的表达条件，开发了一种高效层析纯化策略，验证了重组蛋白的活性，并应用于高通量筛选靶向Wnt通路的抑制剂。
3. **文献名称**：*DVL3 phosphorylation dynamics mediated by recombinant protein interaction studies*
**作者**：Wang, H., et al.
**摘要**：利用重组DVL3蛋白进行体外激酶实验，揭示了CK1ε和PKA对其不同结构域的磷酸化调控模式，阐明了磷酸化修饰对DVL3在胚胎发育中功能的影响。
---
**建议**：如需具体文献，可通过PubMed或Google Scholar检索关键词“DVL3 recombinant protein”或“Dishevelled-3 expression”，并筛选近年的功能研究、结构分析或疾病关联论文。
**Background of DVL3 Recombinant Protein**
DVL3 (Dishevelled segment polarity protein 3) is a key component of the Wnt signaling pathway, an evolutionarily conserved system critical for embryonic development, tissue homeostasis, and cell fate determination. As a member of the Dishevelled (DVL) family, which includes DVL1. DVL2. and DVL3. it acts as a scaffold protein to mediate signaling cascades in both canonical (β-catenin-dependent) and non-canonical (planar cell polarity and calcium-dependent) Wnt pathways. DVL3 facilitates signal transduction by interacting with receptors like Frizzled and downstream effectors, regulating processes such as cell proliferation, polarity, and differentiation.
Structurally, DVL3 contains three conserved domains: the DIX domain for protein oligomerization, the PDZ domain for mediating protein-protein interactions, and the DEP domain for membrane localization. Dysregulation of DVL3 is linked to developmental disorders and cancers, including colorectal, breast, and prostate cancers, where its overexpression often correlates with tumor progression and metastasis.
Recombinant DVL3 protein is produced using genetic engineering techniques, typically expressed in bacterial (e.g., *E. coli*) or mammalian systems to ensure proper folding and post-translational modifications. This engineered protein retains functional domains, enabling researchers to study its role in Wnt signaling, screen for inhibitors, or analyze interactions with other pathway components (e.g., β-catenin, Axin). Its applications span structural biology, *in vitro* binding assays, and drug discovery, offering insights into targeting DVL3-associated diseases. By elucidating DVL3's mechanisms, recombinant forms contribute to advancing therapeutic strategies for cancers and developmental pathologies driven by Wnt pathway aberrations.
×
