| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYH2 |
| Uniprot No | Q9UKX2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MYH2重组蛋白的3篇参考文献示例(注：文献为虚构示例，仅作参考格式示范)：
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1. **文献名称**：*"Recombinant MYH2 Expression in Insect Cells for Structural Analysis"*
**作者**：T. Suzuki et al.
**摘要**：本研究利用杆状病毒-昆虫细胞表达系统成功制备了重组MYH2蛋白，通过亲和层析纯化后获得高纯度蛋白，并利用冷冻电镜解析其三维结构，为理解MYH2在肌球蛋白复合体中的构效关系提供依据。
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2. **文献名称**：*"Functional Characterization of Human MYH2 Recombinant Protein in ATPase Activity Assays"*
**作者**：M. Rodriguez & J. Park
**摘要**：作者在大肠杆菌中表达并纯化了人源MYH2重组蛋白，体外实验证明其具有显著的ATP酶活性，且该活性受钙离子浓度调节，提示MYH2在快速骨骼肌收缩中的关键作用。
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3. **文献名称**：*"Development of a MYH2 Recombinant Protein-Based Diagnostic Assay for Myopathies"*
**作者**：L. Chen et al.
**摘要**：该研究将重组MYH2蛋白作为抗原，开发出特异性抗体检测方法，用于诊断与MYH2突变相关的先天性肌病，临床样本验证显示其敏感性和特异性均优于传统检测手段。
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(如需真实文献，建议通过PubMed或Web of Science以“MYH2 recombinant protein”为关键词检索。)
MYH2 (myosin heavy chain 2) is a key component of skeletal muscle myosin, a molecular motor essential for muscle contraction. It belongs to the myosin heavy chain family, which comprises multiple isoforms (MYH1. MYH2. MYH4. etc.) expressed in different muscle fiber types. Specifically, MYH2 is predominantly found in fast-twitch type IIa muscle fibers, which exhibit intermediate properties between oxidative slow-twitch (type I) and glycolytic fast-twitch (type IIx/IIb) fibers. These fibers contribute to activities requiring both endurance and rapid force generation.
The MYH2 gene encodes the heavy chain subunit of myosin II, a hexameric protein complex consisting of two heavy chains and four light chains. MYH2 contains critical functional domains, including the motor domain for ATP hydrolysis, the lever arm for force transmission, and the tail region for filament assembly. Its expression is regulated during development, exercise, and pathological conditions, such as muscular dystrophies or aging-related sarcopenia.
Recombinant MYH2 protein is produced using biotechnological systems (e.g., bacterial, insect, or mammalian cell expression) to study its structural, biochemical, and mechanical properties. Purified MYH2 enables in vitro investigations of actin-myosin interactions, ATPase activity, and contractile mechanics, providing insights into muscle physiology and disease mechanisms. Researchers also utilize it to develop therapeutic strategies for neuromuscular disorders or to model mutations linked to hereditary myopathies. Furthermore, recombinant MYH2 serves as an antigen for antibody production in diagnostic assays or as a reference standard in omics studies. Its study bridges molecular biology, biophysics, and clinical research, advancing our understanding of muscle function and dysfunction.
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