| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MYLK4 |
| Uniprot No | Q86YV6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-388aa |
| 氨基酸序列 | MLKVKRLEEFNTCYNSNQLEKMAFFQCREEVEKVKCFLEKNSGDQDSRSR HNEAKEVWSNADLTERMPVKSKRTSALAVDIPAPPAPFDHRIVTAKQGAV NSFYTVSKTEILGGGRFGQVHKCEETATGLKLAAKIIKTRGMKDKEEVKN EISVMNQLDHANLIQLYDAFESKNDIVLVMEYVDGGELFDRIIDESYNLT ELDTILFMKQICEGIRHMHQMYILHLDLKPENILCVNRDAKQIKIIDFGL ARRYKPREKLKVNFGTPEFLAPEVVNYDFVSFPTDMWSVGVIAYMLLSGL SPFLGDNDAETLNNILACRWDLEDEEFQDISEEAKEFISKLLIKEKSWRI SASEALKHPWLSDHKLHSRLNAQKKKNRGSDAQDFVTK |
| 预测分子量 | 70 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于MYLK4重组蛋白的参考文献(基于公开数据模拟，部分信息可能需核实)：
1. **标题**: "Characterization of MYLK4 kinase activity and its role in cell motility"
**作者**: Zhang L, et al.
**摘要**: 研究通过重组表达人源MYLK4蛋白，发现其具有钙离子依赖性激酶活性，并证明其在调控非肌肉肌球蛋白II磷酸化中的作用，可能影响肿瘤细胞迁移能力。
2. **标题**: "Recombinant MYLK4 production in E. coli and structural analysis of its catalytic domain"
**作者**: Tanaka K, et al.
**摘要**: 报道了在大肠杆菌中高效表达并纯化MYLK4激酶结构域的方法，通过X射线晶体学解析其三维结构，揭示了ATP结合口袋的独特构象。
3. **标题**: "MYLK4 variants alter smooth muscle contractility via RhoA/ROCK signaling"
**作者**: Chen X, et al.
**摘要**: 利用重组MYLK4蛋白进行体外磷酸化实验，发现其基因突变体可异常激活RhoA通路，可能与血管发育异常相关，为相关疾病机制提供新见解。
注：以上文献信息为模拟创作，实际研究需通过PubMed或Web of Science等平台检索(截至2023年10月，MYLK4相关研究仍较少)。建议结合UniProt数据库(ID: Q86YV5)获取基础蛋白信息。
MYLK4 (myosin light chain kinase 4) is a member of the myosin light chain kinase (MLCK) family, which plays a crucial role in regulating cellular processes such as cytoskeletal dynamics, cell motility, and contractility. Unlike its well-characterized paralogs (e.g., MYLK1-3), MYLK4 is a relatively understudied calcium/calmodulin-independent kinase. It contains a conserved serine/threonine kinase domain but lacks the canonical regulatory domains found in other MLCKs, suggesting distinct mechanisms of activation and substrate targeting. The gene encoding MYLK4 is located on human chromosome 6 and is expressed in various tissues, including the brain, heart, and skeletal muscle, though its physiological roles remain poorly defined.
Emerging studies implicate MYLK4 in cancer progression, particularly in regulating tumor cell invasion and metastasis. It is hypothesized to phosphorylate non-muscle myosin light chains, influencing actomyosin contractility and cellular adhesion. Additionally, MYLK4 has been linked to cardiovascular pathologies, with polymorphisms associated with hypertension and aortic aneurysm risk. However, mechanistic insights remain limited, partly due to the lack of purified, functional protein for biochemical and structural studies.
Recombinant MYLK4 protein, typically produced in bacterial or mammalian expression systems with affinity tags (e.g., His-tag), enables researchers to explore its enzymatic activity, substrate specificity, and interactions. Its production involves codon optimization, solubilization optimization, and purification via chromatography. This tool is vital for high-throughput drug screening, structural biology (e.g., crystallography), and functional assays to decipher its role in disease pathways. Challenges persist in achieving proper post-translational modifications and resolving autoinhibitory regulatory mechanisms. Further research using recombinant MYLK4 could clarify its biological significance and therapeutic potential, bridging gaps in our understanding of MLCK family diversity.
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