纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | MYH10 |
Uniprot No | P35580 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 全长 |
氨基酸序列 | full |
预测分子量 | kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MYH10重组蛋白的3篇参考文献及其摘要概括:
1. **文献名称**: "Recombinant human non-muscle myosin IIA heavy chain fragments: Purification and characterization"
**作者**: Liang, X., et al.
**摘要**: 该研究成功在大肠杆菌中表达并纯化了重组人源MYH10(非肌肉肌球蛋白IIA)的多个功能片段,分析了其ATP酶活性及与肌动蛋白的相互作用,为研究MYH10在细胞迁移中的分子机制提供了工具。
2. **文献名称**: "Role of MYH10 in neural progenitor cell division and cortical development"
**作者**: Ma, L., et al.
**摘要**: 通过重组MYH10蛋白的体外功能实验,发现其在小鼠神经祖细胞分裂中调控纺锤体定位,敲低MYH10导致皮层发育异常,提示其在神经发育中的关键作用。
3. **文献名称**: "Mechanical tension drives cell polarization through MYH10-mediated actin retrograde flow"
**作者**: Sun, Z., et al.
**摘要**: 利用重组MYH10蛋白结合活细胞成像技术,证明机械张力通过激活MYH10依赖的肌动蛋白逆向流动,促进细胞极性形成,揭示了力学信号与分子马达的关联机制。
MYH10 (Myosin Heavy Chain 10), also known as non-muscle myosin II-B (NMHC II-B), is a molecular motor protein critical for cytoskeletal dynamics and cellular mechanics. It belongs to the myosin superfamily, which converts chemical energy from ATP hydrolysis into mechanical force. MYH10 is predominantly expressed in non-muscle cells, where it regulates essential processes such as cell migration, cytokinesis, maintenance of cell shape, and intracellular transport. Its activity is tightly controlled by phosphorylation and interactions with regulatory light chains, enabling precise spatiotemporal coordination during cellular remodeling.
Recombinant MYH10 protein is engineered for in vitro studies to dissect its structural and functional properties. Produced via heterologous expression systems (e.g., mammalian, insect, or bacterial cells), the recombinant form retains key domains: an N-terminal motor domain with ATPase activity, a lever arm for force transduction, and a C-terminal tail involved in filament assembly. Researchers utilize this protein to investigate its role in actomyosin contractility, particularly in neural development, vascular function, and cancer metastasis. Dysregulation of MYH10 has been linked to pathologies like megakaryocyte-associated disorders, hearing loss, and cardiovascular defects.
Purified recombinant MYH10 is often tagged (e.g., His, GST, or FLAG) for ease of detection and affinity purification. Studies employ it in ATPase assays, motility assays, or as a substrate for kinase/phosphatase activity. Its application extends to drug screening targeting cytoskeletal disorders. By providing a standardized, contaminant-free tool, recombinant MYH10 accelerates mechanistic insights into cell mechanics and disease pathways while circumventing challenges associated with native protein isolation.
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