纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | MYH1 |
Uniprot No | P12882 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 全长 |
氨基酸序列 | full |
预测分子量 | kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MYH1重组蛋白的3篇参考文献示例(注:以下文献为虚构示例,仅用于展示格式,实际文献需通过学术数据库查询):
1. **文献名称**: "Expression and Functional Characterization of Recombinant MYH1 in Mammalian Cells"
**作者**: Smith A, et al.
**摘要**: 本研究成功在哺乳动物细胞中表达了重组MYH1蛋白,并验证了其ATP酶活性及与肌动蛋白的相互作用,为研究骨骼肌收缩机制提供了工具。
2. **文献名称**: "Structural Analysis of MYH1 Recombinant Protein Using Cryo-EM"
**作者**: Jones B, et al.
**摘要**: 通过冷冻电镜技术解析了重组MYH1蛋白的三维结构,揭示了其马达结构域的关键构象变化,为理解肌球蛋白动力机制提供结构基础。
3. **文献名称**: "MYH1 Mutations and Their Impact on Recombinant Protein Stability"
**作者**: Chen C, et al.
**摘要**: 分析了致病性MYH1基因突变对重组蛋白稳定性和聚合能力的影响,发现特定突变导致蛋白降解加速,可能与肌病发病相关。
如需真实文献,建议访问PubMed或Google Scholar,搜索关键词“MYH1 recombinant protein expression”或“MYH1 structural/functional study”。
**Background of MYH1 Recombinant Protein**
The MYH1 gene encodes myosin heavy chain 1. a critical component of skeletal muscle myosin, a molecular motor essential for muscle contraction. As part of the myosin II family, MYH1 forms the core of thick filaments in striated muscle, interacting with actin to generate force through ATP hydrolysis. Its structure includes an N-terminal motor domain (responsible for ATPase activity and actin binding), a neck region with light-chain-binding sites for force modulation, and a C-terminal tail mediating filament assembly.
MYH1 is predominantly expressed in fast-twitch skeletal muscle fibers, influencing contractile speed and metabolic efficiency. Mutations in MYH1 are linked to myopathies and cardiomyopathies, driving research into its role in disease. Recombinant MYH1 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables mechanistic studies without native tissue limitations. Production involves cloning the MYH1 cDNA into expression vectors, optimizing conditions for solubility, and purifying using affinity chromatography or tag-based methods.
Applications include *in vitro* studies of muscle contraction, drug screening for neuromuscular disorders, structural analysis (e.g., cryo-EM), and exploring MYH1 mutations' pathological impacts. Challenges persist in expressing full-length MYH1 due to its large size (~220 kDa) and post-translational modifications. Nonetheless, recombinant MYH1 remains vital for advancing muscle biology and therapeutic development.
×