| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITSN1 |
| Uniprot No | Q15811 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1721aa |
| 氨基酸序列 | MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP |
| 预测分子量 | 195,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ITSN1重组蛋白的3篇模拟参考文献示例(实际文献需通过学术数据库查询确认)：
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1. **文献名称**：*Structural characterization of ITSN1 EH domains and their role in endocytosis*
**作者**：Pucharcos, C. et al.
**摘要**：本研究通过重组表达人源ITSN1的EH结构域，利用X射线晶体学解析其三维结构，揭示了EH结构域与内吞相关蛋白(如EGFR)的特异性结合机制，为阐明ITSN1调控细胞内吞的分子基础提供依据。
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2. **文献名称**：*ITSN1 recombinant protein modulates amyloid precursor protein processing via Cdc42 signaling*
**作者**：O’Bryan, J.P. et al.
**摘要**：通过体外实验证实，重组ITSN1蛋白通过激活Cdc42通路，影响γ-分泌酶对淀粉样前体蛋白(APP)的切割，提示其在阿尔茨海默病病理中的潜在调控作用。
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3. **文献名称**：*Expression and functional analysis of ITSN1-S in neuronal differentiation*
**作者**：Sengar, A.S. et al.
**摘要**：在大肠杆菌中重组表达神经元特异性剪接变体ITSN1-S，发现其通过促进N-WASP/Arp2/3复合体介导的肌动蛋白重塑，调控神经突起的生长，为神经发育机制研究提供新方向。
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**备注**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台以关键词“ITSN1 recombinant protein”或“Intersectin 1 expression”检索确认。建议结合具体研究需求筛选近五年高影响力论文。
**Background of ITSN1 Recombinant Protein**
Intersectin-1 (ITSN1) is a multidomain scaffolding protein encoded by the *ITSN1* gene, playing pivotal roles in regulating endocytosis, intracellular signaling, and cytoskeletal dynamics. Structurally, ITSN1 contains two N-terminal Eps15 homology (EH) domains, a central coiled-coil region, and five C-terminal Src homology 3 (SH3) domains. These domains enable ITSN1 to interact with diverse partners, such as clathrin, dynamin, synaptojanin, and small GTPases (e.g., Cdc42), facilitating its involvement in clathrin-mediated endocytosis (CME) and receptor tyrosine kinase signaling.
The recombinant ITSN1 protein is engineered to study its biochemical and functional properties *in vitro*. Produced using expression systems like *E. coli* or mammalian cells, recombinant ITSN1 retains the ability to bind partner proteins, making it invaluable for dissecting molecular mechanisms of endocytosis, vesicle trafficking, and signal transduction. Researchers employ it in pull-down assays, structural studies, and reconstituting protein interaction networks.
ITSN1 is implicated in neurological and oncogenic processes. Dysregulation of ITSN1 has been linked to neurodegenerative disorders (e.g., Alzheimer’s disease) due to its role in synaptic vesicle recycling, and to cancers via its interaction with Ras and other oncogenic pathways. Recombinant ITSN1 aids in screening therapeutic agents targeting these diseases. Additionally, splice variants of ITSN1 (long and short isoforms) exhibit tissue-specific functions, with the long isoform predominantly expressed in neurons.
Overall, ITSN1 recombinant protein serves as a critical tool for unraveling cellular trafficking pathways and developing strategies to modulate ITSN1-associated pathologies.
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