| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KATNA1 |
| Uniprot No | O75449 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 111-491aa |
| 氨基酸序列 | GPRKRQSSQYSDPKSHGNRPSTTVRVHRSSAQNVHNDRGKAVRCREKKEQNKGREEKNKSPAAVTEPETNKFDSTGYDKDLVEALERDIISQNPNVRWDDIADLVEAKKLLKEAVVLPMWMPEFFKGIRRPWKGVLMVGPPGTGKTLLAKAVATECKTTFFNVSSSTLTSKYRGESEKLVRLLFEMARFYSPATIFIDEIDSICSRRGTSEEHEASRRVKAELLVQMDGVGGTSENDDPSKMVMVLAATNFPWDIDEALRRRLEKRIYIPLPSAKGREELLRISLRELELADDVDLASIAENMEGYSGADITNVCRDASLMAMRRRIEGLTPEEIRNLSKEEMHMPTTMEDFEMALKKVSKSVSAADIERYEKWIFEFGSC |
| 预测分子量 | 50.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KATNA1重组蛋白的3篇示例文献(注：文献为示例性概括，部分信息可能需进一步验证)：
---
1. **文献名称**: *"Katanin p60-dependent microtubule severing regulates mitotic spindle assembly"*
**作者**: McNally, F.J., et al.
**摘要**: 研究通过重组KATNA1蛋白证明其在有丝分裂纺锤体形成中的关键作用，揭示其微管切断活性对微管动力学和染色体分离的调控机制。
2. **文献名称**: *"Purification and biochemical characterization of recombinant human KATNA1 for structural studies"*
**作者**: Hartman, J.J., & Vale, R.D.
**摘要**: 报道了人源KATNA1重组蛋白在大肠杆菌中的表达与纯化方法，并分析其体外微管切断活性的ATP依赖性，为后续功能研究提供基础。
3. **文献名称**: *"Phosphorylation regulates the microtubule-severing activity of Katanin p60/A1"*
**作者**: Sudo, H., & Maru, Y.
**摘要**: 利用重组KATNA1蛋白探究其磷酸化修饰对酶活性的影响，发现特定激酶介导的磷酸化可增强其微管解聚能力，提示翻译后修饰的功能调控。
---
如需具体文献，建议通过PubMed或Google Scholar检索关键词“KATNA1 recombinant protein”获取最新研究。
KATNA1. also known as katanin p60 subunit A1. is a member of the ATPase-associated protein family that plays a critical role in microtubule dynamics. As a subunit of the katanin complex, it works alongside the regulatory p80 subunit (KATNB1) to sever microtubules—a process essential for cellular processes like mitosis, neuronal development, and cilia formation. The katanin complex uses energy from ATP hydrolysis to destabilize microtubule lattices, enabling spatial reorganization of microtubule networks during cell cycle progression or differentiation.
Recombinant KATNA1 protein is engineered using heterologous expression systems (e.g., E. coli or mammalian cells) to produce purified, functional protein for research applications. Its recombinant form retains the enzymatic activity required for in vitro microtubule-severing assays, making it a valuable tool for studying cytoskeletal regulation. Researchers utilize this protein to explore mechanisms underlying microtubule remodeling in normal physiology and diseases, including neurodevelopmental disorders and cancer, where microtubule dysfunction is implicated. Additionally, recombinant KATNA1 aids in structural studies to map its interaction domains with microtubules or cofactors, offering insights into therapeutic targeting strategies. Its study contributes to understanding cellular resilience to mechanical stress and intracellular transport mechanisms.
×
