| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHRD |
| Uniprot No | Q9H2X0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-995aa |
| 氨基酸序列 | MPSLPAPPAPLLLLGLLLLGSRPARGAGPEPPVLPIRSEKEPLPVRGAAGCTFGGKVYALDETWHPDLGEPFGVMRCVLCACEAPQWGRRTRGPGRVSCKNIKPECPTPACGQPRQLPGHCCQTCPQERSSSERQPSGLSFEYPRDPEHRSYSDRGEPGAEERARGDGHTDFVALLTGPRSQAVARARVSLLRSSLRFSISYRRLDRPTRIRFSDSNGSVLFEHPAAPTQDGLVCGVWRAVPRLSLRLLRAEQLHVALVTLTHPSGEVWGPLIRHRALAAETFSAILTLEGPPQQGVGGITLLTLSDTEDSLHFLLLFRGLLEPRSGGLTQVPLRLQILHQGQLLRELQANVSAQEPGFAEVLPNLTVQEMDWLVLGELQMALEWAGRPGLRISGHIAARKSCDVLQSVLCGADALIPVQTGAAGSASLTLLGNGSLIYQVQVVGTSSEVVAMTLETKPQRRDQRTVLCHMAGLQPGGHTAVGICPGLGARGAHMLLQNELFLNVGTKDFPDGELRGHVAALPYCGHSARHDTLPVPLAGALVLPPVKSQAAGHAWLSLDTHCHLHYEVLLAGLGGSEQGTVTAHLLGPPGTPGPRRLLKGFYGSEAQGVVKDLEPELLRHLAKGMASLMITTKGSPRGELRGQVHIANQCEVGGLRLEAAGAEGVRALGAPDTASAAPPVVPGLPALAPAKPGGPGRPRDPNTCFFEGQQRPHGARWAPNYDPLCSLCTCQRRTVICDPVVCPPPSCPHPVQAPDQCCPVCPEKQDVRDLPGLPRSRDPGEGCYFDGDRSWRAAGTRWHPVVPPFGLIKCAVCTCKGGTGEVHCEKVQCPRLACAQPVRVNPTDCCKQCPVGSGAHPQLGDPMQADGPRGCRFAGQWFPESQSWHPSVPPFGEMSCITCRCGAGVPHCERDDCSLPLSCGSGKESRCCSRCTAHRRPAPETRTDPELEKEAEGS |
| 预测分子量 | 102 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CHRD(Chordin)重组蛋白的3篇参考文献，包含文献名称、作者及摘要概述：
1. **"Recombinant Chordin inhibits BMP signaling and promotes cartilage differentiation"**
*作者：Zhang Y. et al. (2004)*
摘要：研究报道了重组Chordin蛋白的高效表达及纯化方法，证实其通过结合BMP分子抑制下游信号通路，并在体外实验中促进间充质干细胞向软骨细胞分化。
2. **"Structural basis of BMP signaling inhibition by Chordin"**
*作者：Troilo H. et al. (2014)*
摘要：通过X射线晶体学解析了Chordin-BMP复合物的三维结构，揭示了Chordin通过特定结构域竞争性抑制BMP与受体结合的分子机制，为开发靶向BMP信号通路的治疗策略提供依据。
3. **"Chordin recombinant protein enhances nerve regeneration in zebrafish models"**
*作者：Smith J.R. & Beck C.W. (2018)*
摘要：利用重组Chordin蛋白处理斑马鱼神经损伤模型，发现其通过调节BMP/TGF-β信号通路显著促进轴突再生，提示Chordin在神经修复治疗中的潜在应用价值。
备注：以上文献信息为示例性内容，实际引用时建议通过PubMed或Web of Science核对真实文献。Chordin相关研究可重点关注发育生物学领域，关键词包括"Chordin recombinant expression/BMP antagonist/tissue morphogenesis"。
**Background of CHRD (Chordin) Recombinant Protein**
Chordin (CHRD) is a secreted glycoprotein crucial in embryonic development, particularly in establishing the dorsal-ventral axis. It acts as a key antagonist of bone morphogenetic proteins (BMPs), growth factors that regulate cell differentiation and tissue patterning. By binding directly to BMPs (e.g., BMP2. BMP4), Chordin inhibits their interaction with receptors, thereby modulating BMP signaling gradients essential for proper neural induction, organogenesis, and skeletal formation. This regulatory mechanism is conserved across species, with homologs identified in vertebrates and invertebrates.
Structurally, Chordin contains multiple cysteine-rich (CR) domains that mediate BMP binding. Its activity is further regulated by extracellular proteases, such as Tolloid, which cleave Chordin to release BMPs, fine-tuning signaling dynamics. Dysregulation of Chordin-BMP interactions is linked to developmental disorders, including skeletal malformations and congenital heart defects, underscoring its biological significance.
Recombinant Chordin protein is produced using expression systems like *E. coli* or mammalian cells, enabling scalable synthesis for research and therapeutic applications. It retains the native protein's ability to bind BMPs, making it a valuable tool for studying developmental pathways, tissue engineering, and disease models. For instance, recombinant Chordin is used to investigate BMP-driven processes in cancer, fibrosis, and bone regeneration.
In drug discovery, Chordin-derived peptides or mimics are explored for modulating BMP signaling in conditions with excessive BMP activity, such as fibrodysplasia ossificans progressiva. Quality-controlled batches of recombinant Chordin are validated via SDS-PAGE, Western blotting, and functional assays (e.g., BMP inhibition in cell cultures), ensuring consistency for experimental and preclinical use.
Overall, Chordin recombinant protein serves as a pivotal reagent in developmental biology and regenerative medicine, bridging mechanistic insights with therapeutic potential.
×
