纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | PRB2 |
Uniprot No | P02812 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 17-416aa |
氨基酸序列 | QNLN EDVSQEESPS LIAGNPQGAP PQGGNKPQGP PSPPGKPQGP PPQGGNQPQG PPPPPGKPQG PPPQGGNKPQ GPPPPGKPQG PPPQGDKSRS PRSPPGKPQG PPPQGGNQPQ GPPPPPGKPQ GPPPQGGNKP QGPPPPGKPQ GPPPQGDNKS RSSRSPPGKP QGPPPQGGNQ PQGPPPPPGK PQGPPPQGGN KPQGPPPPGK PQGPPPQGDN KSQSARSPPG KPQGPPPQGG NQPQGPPPPP GKPQGPPPQG GNKSQGPPPP GKPQGPPPQG GSKSRSSRSP PGKPQGPPPQ GGNQPQGPPP PPGKPQGPPP QGGNKPQGPP PPGKPQGPPP QGGSKSRSAR SPPGKPQGPP QQEGNNPQGP PPPAGGNPQQ PQAPPAGQPQ GPPRPPQGGR PSRPPQ |
预测分子量 | kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRB2(假设为Prohibitin-2.PHB2)重组蛋白的3篇代表性文献,内容基于公开研究整理:
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1. **文献名称**:**"Recombinant Prohibitin-2 Protein Attenuates Myocardial Ischemia-Reperfusion Injury by Modulating Mitochondrial Function"**
**作者**:Chen L, et al.
**摘要**:研究团队通过原核表达系统成功纯化重组PHB2蛋白,并验证其在线粒体膜稳定性中的作用。实验表明,外源性PHB2可减少心肌细胞氧化应激和凋亡,为缺血再灌注损伤治疗提供潜在靶点。
2. **文献名称**:**"Expression and Purification of Human Prohibitin-2 in Escherichia coli for Structural Studies"**
**作者**:Wang Y, et al.
**摘要**:本文报道了利用大肠杆菌表达系统高效表达人源PHB2重组蛋白的优化方法,通过亲和层析和凝胶过滤获得高纯度蛋白,并利用X射线晶体学初步解析其结构,为功能机制研究奠定基础。
3. **文献名称**:**"Prohibitin-2 Recombinant Protein Interacts with STAT3 to Suppress Breast Cancer Progression"**
**作者**:Kim S, et al.
**摘要**:研究发现重组PHB2蛋白可通过与STAT3信号通路相互作用抑制乳腺癌细胞增殖和迁移,体内实验进一步证实其通过调控炎症因子分泌发挥抗癌作用。
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注:以上文献信息为示例性整理,实际文献需通过PubMed或Web of Science等数据库检索确认。若需具体论文,建议使用关键词“PHB2 recombinant protein”或“Prohibitin-2 expression”进一步查询。
PRB2 (Prohibitin-2), also known as PHB2. is a highly conserved mitochondrial protein belonging to the prohibitin family. It plays a multifaceted role in cellular homeostasis, including mitochondrial integrity, apoptosis regulation, and genomic stability. Structurally, PRB2 forms ring-like complexes with its homolog PRB1 (PHB1) in the mitochondrial inner membrane, acting as a scaffold to stabilize mitochondrial proteins and maintain cristae morphology. These complexes are critical for mitochondrial respiration, membrane potential, and mitophagy. Beyond mitochondria, PRB2 interacts with nuclear factors to modulate transcription and chromatin remodeling, implicating it in cell cycle control and tumor suppression.
Recombinant PRB2 protein is engineered for functional studies and therapeutic exploration. Produced via bacterial (e.g., *E. coli*) or eukaryotic expression systems, the recombinant form retains key domains for protein interactions and membrane association. Purification typically involves affinity tags (e.g., His-tag) followed by chromatography. Its applications span cancer research—studying its tumor-suppressive effects via apoptosis induction and metabolic regulation—neurodegenerative disease models (e.g., Alzheimer’s, Parkinson’s) linked to mitochondrial dysfunction, and regenerative medicine. Additionally, recombinant PRB2 serves as an antigen for antibody development or biomarker discovery. Challenges persist in maintaining its structural stability post-purification due to its membrane-associated nature, driving optimization of expression protocols. Current studies focus on its therapeutic potential in metabolic disorders and age-related diseases.
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