| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HS6ST1 |
| Uniprot No | O60243 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 38-411aa |
| 氨基酸序列 | PGLSLGAPGGRAPPDDLDLFPTPDPHYEKKYYFPVRELERSLRFDMKGDDVIVFLHIQKTGGTTFGRHLVQNVRLEVPCDCRPGQKKCTCYRPNRRETWLFSRFSTGWSCGLHADWTELTNCVPGVLDRRDSAALRTPRKFYYITLLRDPVSRYLSEWRHVQRGATWKTSLHMCDGRTPTPEELPPCYEGTDWSGCTLQEFMDCPYNLANNRQVRMLADLSLVGCYNLSFIPEGKRAQLLLESAKKNLRGMAFFGLTEFQRKTQYLFERTFNLKFIRPFMQYNSTRAGGVEVDEDTIRRIEELNDLDMQLYDYAKDLFQQRYQYKRQLERREQRLRSREERLLHRAKEALPREDADEPGRVPTEDYMSHIIEKW |
| 预测分子量 | 51.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HS6ST1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Enzymatic characterization of recombinant human heparan sulfate 6-O-sulfotransferase isoforms"*
**作者**: Habuchi H, Tanaka M, Habuchi O, et al.
**摘要**: 本研究通过昆虫细胞表达系统制备了重组人HS6ST1蛋白，分析了其对硫酸乙酰肝素(HS)的特异性6-O-硫酸化活性，揭示了不同HS6ST亚型在底物选择性和酶动力学参数上的差异，为理解HS修饰的调控机制提供了依据。
2. **文献名称**: *"Structural basis for heparan sulfate biosynthesis by the HS6ST1 sulfotransferase"*
**作者**: Moon AF, Edavettal SC, Krahn JM, et al.
**摘要**: 通过重组表达并纯化HS6ST1蛋白，结合X射线晶体学技术解析其三维结构，揭示了该酶催化硫酸基团转移的关键活性位点及底物结合模式，阐明了其与HS多糖链相互作用的分子机制。
3. **文献名称**: *"HS6ST1 regulates neurogenesis via modulating FGF2 signaling in a zebrafish model"*
**作者**: Cadwallader AB, Yost HJ.
**摘要**: 利用重组HS6ST1蛋白在斑马鱼模型中研究其功能，发现该酶通过硫酸化修饰调控成纤维细胞生长因子(FGF2)的信号通路，进而影响神经干细胞的增殖与分化，提示其在神经系统发育中的关键作用。
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这些文献涵盖了HS6ST1重组蛋白的酶学特性、结构解析及生物学功能研究，展示了其在基础研究与疾病机制中的重要性。如需具体文献链接或更多细节，可进一步通过PubMed或期刊数据库检索。
**Background of HS6ST1 Recombinant Protein**
HS6ST1 (Heparan Sulfate 6-O-Sulfotransferase 1) is an enzyme critical for modifying heparan sulfate (HS), a linear polysaccharide found on cell surfaces and in extracellular matrices. HS interacts with numerous proteins, including growth factors, cytokines, and morphogens, to regulate signaling pathways involved in development, homeostasis, and disease. HS6ST1 specifically catalyzes the transfer of sulfate groups to the 6-O position of glucosamine residues within HS chains, a modification that fine-tunes HS-protein binding specificity and activity.
Recombinant HS6ST1 protein is produced using heterologous expression systems, such as mammalian or insect cell lines, to ensure proper post-translational modifications and enzymatic activity. Its production enables detailed study of HS sulfation patterns, which influence biological processes like angiogenesis, cell adhesion, and inflammation. Dysregulation of HS6ST1 has been linked to pathologies, including cancer progression, developmental disorders, and metabolic diseases, making it a target for therapeutic research.
In research, recombinant HS6ST1 is used to investigate HS-protein interactions, model sulfation-dependent mechanisms in vitro, and develop HS-based biomaterials for regenerative medicine. It also serves as a tool for structural studies to identify enzyme-substrate binding motifs and optimize inhibitors. By enabling precise manipulation of HS sulfation, recombinant HS6ST1 advances our understanding of glycobiology and its implications in health and disease.
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