| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLSCR2 |
| Uniprot No | Q9NRY7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-297aa |
| 氨基酸序列 | MRSWNSLFCLNSSRPPGHIVYPKHQAGHTGKQADHLGSQAFYPGRQHDYLVPPAGTAGIPVQNQPGRPEGVPWMPAPPPPLNCPPGLEYLSQIDMILIHQQIELLEVLFSFESSNMYEIKNSFGQRIYFAAEDTNFCIRNCCGRSRPFTLRITDNVGREVITLERPLRCNCCCCPCCLQEIEIQAPPGVPVGYVTQTWHPCLTKFTIKNQKREDVLKISGPCIVCSCIAGVDFEITSLDEQIVVGRISKHWSGFLREAFTDADNFGIQFPRDLDVKMKAVMIGACFLIDYMFFERTR |
| 预测分子量 | 33,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLSCR2重组蛋白的3篇参考文献，按文献名称、作者和摘要内容概括整理：
1. **"Expression and Functional Analysis of Recombinant Phospholipid Scramblase 2 (PLSCR2) in Immune Cells"**
- **作者**: Zhang Y, Chen L, et al.
- **摘要**: 本研究通过大肠杆菌系统表达并纯化了重组PLSCR2蛋白，揭示了其在巨噬细胞中的脂质转运功能及对炎症信号通路的调控作用，表明PLSCR2可能通过影响细胞膜磷脂不对称性参与免疫应答。
2. **"Structural Insights into PLSCR2-Mediated Lipid Scrambling by Cryo-EM"**
- **作者**: Wang H, Li J, et al.
- **摘要**: 利用重组PLSCR2蛋白进行冷冻电镜结构解析，阐明了其跨膜结构域如何介导磷脂分子的双向翻转，为理解PLSCR2在细胞凋亡和血小板活化中的分子机制提供了结构基础。
3. **"PLSCR2 Regulates EGFR Signaling through Interaction with Membrane Phospholipids"**
- **作者**: Gupta S, Rivera-Correa J, et al.
- **摘要**: 通过哺乳动物细胞表达重组PLSCR2.发现其与表皮生长因子受体(EGFR)的共定位及对EGFR内吞和信号传导的调控作用，提示PLSCR2可能通过改变膜微环境影响受体酪氨酸激酶活性。
---
**备注**：以上文献信息为示例，实际引用时需根据具体研究内容核对数据库(如PubMed、Web of Science)以获取准确信息。
**Background of PLSCR2 Recombinant Protein**
Phospholipid scramblase 2 (PLSCR2) is a member of the scramblase protein family, which mediates the rapid, calcium-dependent translocation of phospholipids between the inner and outer leaflets of cell membranes. This process is critical for maintaining membrane asymmetry and facilitating cellular functions such as apoptosis, blood coagulation, and immune signaling. PLSCR2. encoded by the *PLSCR2* gene, is primarily expressed in immune cells, including lymphocytes and platelets, and has been implicated in immune regulation and inflammatory responses. Unlike its well-studied homolog PLSCR1. which localizes to the plasma membrane, PLSCR2 is predominantly found in intracellular compartments, suggesting distinct roles in cellular physiology.
Recombinant PLSCR2 protein is produced using genetic engineering techniques, often in bacterial or mammalian expression systems, to enable functional and structural studies. Its production typically involves cloning the *PLSCR2* gene into an expression vector, followed by purification via affinity chromatography (e.g., His-tag systems). The recombinant protein retains the ability to bind phospholipids and interact with signaling molecules, making it a valuable tool for investigating scramblase activity, membrane dynamics, and intracellular signaling pathways.
Research on PLSCR2 has highlighted its potential involvement in autoimmune diseases, cancer, and viral infections. For example, studies suggest PLSCR2 may regulate cytokine secretion and influence apoptosis in malignant cells. Additionally, its interaction with viral proteins underscores its role in host-pathogen interactions. Despite these advances, the precise molecular mechanisms and physiological relevance of PLSCR2 remain under investigation. Recombinant PLSCR2 continues to serve as a critical reagent for elucidating its biological functions and therapeutic potential in disease contexts.
×
