| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HS2ST1 |
| Uniprot No | Q7LGA3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-356aa |
| 氨基酸序列 | MGLLRIMMPPKLQLLAVVAFAVAMLFLENQIQKLEESRSKLERAIARHEVREIEQRHTMDGPRQDATLDEEEDMVIIYNRVPKTASTSFTNIAYDLCAKNKYHVLHINTTKNNPVMSLQDQVRFVKNITSWKEMKPGFYHGHVSYLDFAKFGVKKKPIYINVIRDPIERLVSYYYFLRFGDDYRPGLRRRKQGDKKTFDECVAEGGSDCAPEKLWLQIPFFCGHSSECWNVGSRWAMDQAKYNLINEYFLVGVTEELEDFIMLLEAALPRFFRGATELYRTGKKSHLRKTTEKKLPTKQTIAKLQQSDIWKMENEFYEFALEQFQFIRAHAVREKDGDLYILAQNFFYEKIYPKSN |
| 预测分子量 | 41,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HS2ST1重组蛋白的3篇参考文献的简要信息(注：文献为示例性内容，实际引用需核实原文)：
---
1. **文献名称**: "Functional characterization of recombinant human heparan sulfate 2-O-sulfotransferase (HS2ST1) in glycosaminoglycan biosynthesis"
**作者**: Chen Y, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了功能性重组HS2ST1蛋白，证实其能够催化硫酸肝素(HS)链的2-O-磺酸化修饰，并揭示其底物特异性对细胞信号通路(如FGF2信号)的影响。
2. **文献名称**: "Structural insights into HS2ST1-mediated sulfation patterning and its role in Wnt signaling regulation"
**作者**: Smith J, et al.
**摘要**: 通过X射线晶体学解析了重组HS2ST1蛋白的三维结构，发现其活性位点关键氨基酸对硫酸转移的调控机制，并证明HS2ST1通过修饰Wnt蛋白的硫酸肝素结合域影响胚胎发育过程。
3. **文献名称**: "HS2ST1 overexpression alters extracellular matrix remodeling and promotes cancer metastasis"
**作者**: Lee H, et al.
**摘要**: 利用哺乳动物细胞表达系统制备重组HS2ST1.发现其过表达通过增强肿瘤细胞表面HS的2-O-磺酸化水平，促进整合素信号通路激活，从而驱动乳腺癌细胞侵袭和转移。
---
如需具体文献，建议在PubMed或Google Scholar中检索关键词(如"HS2ST1 recombinant" "heparan sulfate 2-O-sulfotransferase")，并优先选择近年发表的实验研究。
HS2ST1 (heparan sulfate 2-O-sulfotransferase 1) is a key enzyme involved in the post-translational modification of heparan sulfate (HS), a linear polysaccharide critical for regulating cellular signaling and extracellular matrix interactions. This enzyme specifically catalyzes the transfer of a sulfate group to the 2-O position of glucuronic acid residues within HS chains, a modification essential for mediating ligand-receptor interactions. HS2ST1 plays a pivotal role in modulating biological processes such as growth factor signaling (e.g., FGF2. VEGF), cell adhesion, and developmental morphogenesis by shaping the sulfation patterns of HS, which determine its binding specificity to proteins.
Recombinant HS2ST1 protein is produced through genetic engineering, often using mammalian or insect expression systems to ensure proper post-translational modifications and enzymatic activity. Its production enables detailed biochemical studies, including substrate specificity, kinetic analysis, and structural characterization. Researchers utilize this recombinant protein to investigate HS sulfation mechanisms in pathologies such as cancer metastasis, inflammatory disorders, and congenital defects (e.g., renal dysplasias linked to HS2ST1 mutations). Additionally, it serves as a tool for in vitro synthesis of selectively sulfated HS oligosaccharides, which are explored for therapeutic applications, including drug delivery systems and regenerative medicine. The study of HS2ST1 also contributes to understanding developmental biology, as altered HS sulfation patterns disrupt embryogenesis and organogenesis. By providing a controlled source of functional enzyme, recombinant HS2ST1 accelerates research into HS-related molecular pathways and potential therapeutic interventions targeting sulfation-dependent interactions.
×
