| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLD2 |
| Uniprot No | O14939 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-933aa |
| 氨基酸序列 | MTATPESLFPTGDELDSSQLQMESDEVDTLKEGEDPADRMHPFLAIYELQ SLKVHPLVFAPGVPVTAQVVGTERYTSGSKVGTCTLYSVRLTHGDFSWTT KKKYRHFQELHRDLLRHKVLMSLLPLARFAVAYSPARDAGNREMPSLPRA GPEGSTRHAASKQKYLENYLNCLLTMSFYRNYHAMTEFLEVSQLSFIPDL GRKGLEGMIRKRSGGHRVPGLTCCGRDQVCYRWSKRWLVVKDSFLLYMCL ETGAISFVQLFDPGFEVQVGKRSTEARHGVRIDTSHRSLILKCSSYRQAR WWAQEITELAQGPGRDFLQLHRHDSYAPPRPGTLARWFVNGAGYFAAVAD AILRAQEEIFITDWWLSPEVYLKRPAHSDDWRLDIMLKRKAEEGVRVSIL LFKEVELALGINSGYSKRALMLLHPNIKVMRHPDQVTLWAHHEKLLVVDQ VVAFLGGLDLAYGRWDDLHYRLTDLGDSSESAASQPPTPRPDSPATPDLS HNQFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGL PARDLARHFIQRWNFTKTTKAKYKTPIYPYLLPKSTSTANQLPFTLPGGQ CTTVQVLRSVDRWSAGTLENSILNAYLHTIRESQHFLYIENQFFISCSDG RTVLNKVGDEIVDRILKAHKQGWCYRVYVLLPLLPGFEGDISTGGGNSIQ AILHFTYRTLCRGEYSILHRLKAAMGTAWRDYISICGLRTHGELGGHPVS ELIYIHSKVLIADDRTVIIGSANINDRSLLGKRDSELAVLIEDTETEPSL MNGAEYQAGRFALSLRKHCFGVILGANTRPDLDLRDPICDDFFQLWQDMA ESNANIYEQIFRCLPSNATRSLRTLREYVAVEPLATVSPPLARSELTQVQ GHLVHFPLKFLEDESLLPPLGSKEGMIPLEVWT |
| 预测分子量 | 105,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于PLD2重组蛋白的参考文献概要：
1. **《Expression and Purification of Active Human Phospholipase D2 in Baculovirus-Insect Cell System》**
- 作者：Sung, T.C. et al.
- 摘要：报道利用杆状病毒-昆虫细胞系统高效表达人源PLD2重组蛋白，通过亲和层析纯化获得高活性酶，验证其对底物PtdCho的水解能力及信号传导功能。
2. **《Structural Insights into Phospholipase D2 Function through Recombinant Protein Crystallography》**
- 作者：Yoon, M.S. & Frohman, M.A.
- 摘要：解析重组PLD2蛋白的晶体结构，揭示其催化域与磷脂底物结合的关键位点，阐明其膜定位及调控机制，为靶向药物设计提供结构基础。
3. **《Recombinant PLD2 Modulates EGFR Endocytosis via Phosphatidic Acid Production in Cancer Cells》**
- 作者：Gomez-Cambronero, J. et al.
- 摘要：通过重组PLD2过表达实验，证明其生成的磷脂酸(PA)通过调控网格蛋白依赖的内吞途径，促进EGFR信号内化，影响肿瘤细胞迁移。
注：以上文献为示例性概括，实际引用时需核对具体论文内容及作者信息。建议通过PubMed或Web of Science以"PLD2 recombinant"为关键词检索最新研究。
**Background of PLD2 Recombinant Protein**
Phospholipase D2 (PLD2) is a member of the phospholipase D family, a class of enzymes that catalyze the hydrolysis of phosphatidylcholine to generate phosphatidic acid (PA) and choline. PA acts as a lipid second messenger, regulating diverse cellular processes, including membrane trafficking, cytoskeletal reorganization, cell proliferation, and survival. PLD2. encoded by the *PLD2* gene in humans, is ubiquitously expressed and localized to plasma membranes, endosomes, and other membranous compartments. Structurally, PLD2 contains two conserved catalytic HxKxxxxD (HKD) motifs critical for its enzymatic activity.
Recombinant PLD2 protein is produced using genetic engineering techniques, often expressed in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., insect or mammalian cells) to ensure proper folding and post-translational modifications. Its recombinant form enables detailed biochemical studies, such as enzymatic activity assays, inhibitor screening, and interaction mapping with regulatory proteins (e.g., small GTPases, kinases).
PLD2 has garnered attention due to its role in disease pathways. Overactivation of PLD2 is implicated in cancer progression, neuroinflammation, and metabolic disorders, making it a potential therapeutic target. For example, PLD2-derived PA promotes tumor cell invasion by modulating cytoskeletal dynamics or activating oncogenic signaling cascades like mTOR and Raf/MEK/ERK. In neurodegenerative diseases, dysregulated PLD2 activity may contribute to synaptic dysfunction.
Researchers utilize recombinant PLD2 to explore its structure-function relationships, develop isoform-specific inhibitors, and validate cellular pathways. Recent advances in structural biology (e.g., cryo-EM) have further elucidated PLD2's regulatory mechanisms, aiding drug discovery. Overall, PLD2 recombinant protein serves as a vital tool for dissecting lipid-mediated signaling and advancing targeted therapies.
×
