| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RIPK1 |
| Uniprot No | Q13546 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-671aa |
| 氨基酸序列 | MQPDMSLNVI KMKSSDFLES AELDSGGFGK VSLCFHRTQG LMIMKTVYKG PNCIEHNEAL LEEAKMMNRL RHSRVVKLLG VIIEEGKYSL VMEYMEKGNL MHVLKAEMST PLSVKGRIIL EIIEGMCYLH GKGVIHKDLK PENILVDNDF HIKIADLGLA SFKMWSKLNN EEHNELREVD GTAKKNGGTL YYMAPEHLND VNAKPTEKSD VYSFAVVLWA IFANKEPYEN AICEQQLIMC IKSGNRPDVD DITEYCPREI ISLMKLCWEA NPEARPTFPG IEEKFRPFYL SQLEESVEED VKSLKKEYSN ENAVVKRMQS LQLDCVAVPS SRSNSATEQP GSLHSSQGLG MGPVEESWFA PSLEHPQEEN EPSLQSKLQD EANYHLYGSR MDRQTKQQPR QNVAYNREEE RRRRVSHDPF AQQRPYENFQ NTEGKGTAYS SAASHGNAVH QPSGLTSQPQ VLYQNNGLYS SHGFGTRPLD PGTAGPRVWY RPIPSHMPSL HNIPVPETNY LGNTPTMPFS SLPPTDESIK YTIYNSTGIQ IGAYNYMEIG GTSSSLLDST NTNFKEEPAA KYQAIFDNTT SLTDKHLDPI RENLGKHWKN CARKLGFTQS QIDEIDHDYE RDGLKEKVYQ MLQKWVMREG IKGATVGKLA QALHQCSRID LLSSLIYVSQ N |
| 预测分子量 | 108 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RIPK1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"RIPK1 inhibits ZBP1-driven necroptosis during development"*
**作者**：Newton, K. et al.
**摘要**：该研究利用重组RIPK1蛋白分析其在细胞死亡信号中的作用，发现RIPK1通过抑制ZBP1介导的坏死性凋亡维持胚胎发育稳态，揭示了RIPK1激酶活性与非激酶功能的双重调控机制。
2. **文献名称**：*"Structure of the RIPK1 catalytic domain bound to necrostatin-1"*
**作者**：Xie, T. et al.
**摘要**：通过重组RIPK1激酶结构域的晶体结构解析，阐明了小分子抑制剂necrostatin-1与RIPK1结合的分子机制，为靶向RIPK1的药物设计提供了结构基础。
3. **文献名称**：*"RIPK1 ensures intestinal homeostasis by preventing apoptosis and necroptosis"*
**作者**：Dannappel, M. et al.
**摘要**：利用RIPK1重组蛋白及基因敲除模型，证明RIPK1通过抑制caspase-8依赖的凋亡和RIPK3介导的坏死性凋亡，维持肠道上皮细胞稳态，其功能缺失导致肠道炎症和早衰。
(注：以上文献名称和作者为简化示例，实际引用需根据具体文献调整。)
Receptor-interacting protein kinase 1 (RIPK1) is a serine/threonine kinase that plays a pivotal role in regulating cell survival, inflammation, and programmed cell death pathways, including apoptosis and necroptosis. Initially identified as a mediator of TNF receptor 1 (TNFR1) signaling, RIPK1 contains a kinase domain, a death domain, and a RIP homotypic interaction motif (RHIM), enabling its participation in diverse protein complexes. It acts as a molecular switch, directing cellular outcomes toward NF-κB-mediated pro-survival signaling or caspase-dependent apoptosis/necroptosis, depending on post-translational modifications (e.g., ubiquitination, phosphorylation) and cellular context.
Recombinant RIPK1 proteins are engineered versions produced via heterologous expression systems (e.g., E. coli, insect, or mammalian cells) to study its structure-function relationships, enzymatic activity, and interactions. These proteins are critical tools for in vitro assays, such as kinase activity profiling, inhibitor screening, and structural studies (e.g., X-ray crystallography). Researchers use recombinant RIPK1 to dissect its role in diseases like neurodegenerative disorders, autoimmune conditions, and cancer, where dysregulated necroptosis or inflammation is implicated. Notably, RIPK1 inhibitors are under investigation for therapeutic applications, driving demand for high-purity recombinant proteins to evaluate drug specificity and mechanisms. However, challenges remain in preserving native conformational states during recombinant production due to its complex regulatory domains. Advances in protein engineering and post-translational modification mimicry continue to enhance the utility of RIPK1 recombinant proteins in both basic research and drug discovery.
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