| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EPX |
| Uniprot No | P11678 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-715aa |
| 氨基酸序列 | MHLLPALAGVLATLVLAQPCEGTDPASPGAVETSVLRDCIAEAKLLVDAA YNWTQKSIKQRLRSGSASPMDLLSYFKQPVAATRTVVRAADYMHVALGLL EEKLQPQRSGPFNVTDVLTEPQLRLLSQASGCALRDQAERCSDKYRTITG RCNNKRRPLLGASNQALARWLPAEYEDGLSLPFGWTPSRRRNGFLLPLVR AVSNQIVRFPNERLTSDRGRALMFMQWGQFIDHDLDFSPESPARVAFTAG VDCERTCAQLPPCFPIKIPPNDPRIKNQRDCIPFFRSAPSCPQNKNRVRN QINALTSFVDASMVYGSEVSLSLRLRNRTNYLGLLAINQRFQDNGRALLP FDNLHDDPCLLTNRSARIPCFLAGDTRSTETPKLAAMHTLFMREHNRLAT ELRRLNPRWNGDKLYNEARKIMGAMVQIITYRDFLPLVLGKARARRTLGH YRGYCSNVDPRVANVFTLAFRFGHTMLQPFMFRLDSQYRASAPNSHVPLS SAFFASWRIVYEGGIDPILRGLMATPAKLNRQDAMLVDELRDRLFRQVRR IGLDLAALNMQRSRDHGLPGYNAWRRFCGLSQPRNLAQLSRVLKNQDLAR KFLNLYGTPDNIDIWIGAIAEPLLPGARVGPLLACLFENQFRRARDGDRF WWQKRGVFTKRQRKALSRISLSRIICDNTGITTVSRDIFRANIYPRGFVN CSRIPRLNLSAWRGT |
| 预测分子量 | 106 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EPX重组蛋白的3篇参考文献示例，涵盖表达、结构及功能研究：
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1. **标题**: "High-yield expression and purification of recombinant human eosinophil peroxidase in Escherichia coli"
**作者**: Zhang L, et al.
**期刊**: *Protein Expression and Purification* (2018)
**摘要**: 研究报道了通过大肠杆菌系统高效表达人源EPX重组蛋白的优化策略，采用His标签纯化获得高纯度蛋白，并验证其酶活性，为后续功能研究提供可靠工具。
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2. **标题**: "Structural insights into the catalytic mechanism of eosinophil peroxidase through recombinant protein crystallography"
**作者**: Thompson R, et al.
**期刊**: *Journal of Molecular Biology* (2020)
**摘要**: 通过重组EPX蛋白的结晶结构解析，揭示了其底物结合位点及氧化反应机制，阐明EPX与中性粒细胞过氧化物酶的活性差异，为靶向抑制剂设计奠定基础。
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3. **标题**: "Recombinant EPX exacerbates airway inflammation in murine asthma models via ROS-dependent pathways"
**作者**: Chen H, et al.
**期刊**: *Allergy* (2021)
**摘要**: 在小鼠哮喘模型中，外源重组EPX蛋白通过激活活性氧(ROS)信号通路增强Th2炎症反应，提示EPX在过敏性疾病中的潜在治疗靶点价值。
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注：以上文献信息为示例性内容，实际引用需以真实发表的论文为准。建议通过PubMed或Web of Science以关键词“eosinophil peroxidase recombinant”检索最新研究。
**Background of EPX Recombinant Protein**
Eosinophil peroxidase (EPX) is a heme-containing enzyme primarily secreted by eosinophils, a type of immune cell involved in combating parasitic infections and modulating inflammatory responses. As a member of the mammalian peroxidase family, EPX plays a critical role in eosinophil-mediated host defense by catalyzing the production of reactive oxygen species (ROS) and toxic intermediates, such as hypohalous acids, which contribute to pathogen destruction and tissue inflammation. Dysregulated EPX activity is associated with allergic diseases, asthma, and eosinophil-related disorders, making it a key biomarker and therapeutic target.
Recombinant EPX protein is engineered using genetic engineering techniques, typically expressed in heterologous systems like *E. coli*, mammalian cells, or insect cells to ensure proper folding and post-translational modifications. The recombinant form retains the functional characteristics of native EPX, including enzymatic activity and substrate specificity, enabling its use in biochemical assays, diagnostic tools, and drug development.
Research applications of recombinant EPX include elucidating its role in eosinophil biology, studying its interactions with immune modulators, and screening inhibitors for therapeutic purposes. In diagnostics, EPX levels in bodily fluids (e.g., serum, sputum) are measured to assess eosinophilic inflammation in conditions like asthma or eosinophilic esophagitis. Additionally, recombinant EPX aids in developing targeted therapies, such as monoclonal antibodies or small-molecule inhibitors, to mitigate eosinophil-driven tissue damage.
Overall, recombinant EPX serves as a vital tool for advancing understanding of eosinophil function and designing precision treatments for inflammatory diseases. Its production and characterization continue to support both basic research and clinical innovations.
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