| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAMP2 |
| Uniprot No | O60895 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 43-175aa |
| 氨基酸序列 | QPLPTTGTPGSEGGTVKNYETAVQFCWNHYKDQMDPIEKDWCDWAMISRPYSTLRDCLEHFAELFDLGFPNPLAERIIFETHQIHFANCSLVQPTFSDPPEDVLLAMIIAPICLIPFLITLVVWRSKDSEAQA |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAMP2重组蛋白的3篇示例参考文献(注：以下内容为虚构示例，实际文献需通过学术数据库检索)：
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1. **标题**: *"Efficient Expression and Purification of Recombinant RAMP2 in Escherichia coli for Functional Studies"*
**作者**: Smith A, et al.
**摘要**: 本研究报道了在大肠杆菌中高效表达可溶性RAMP2重组蛋白的优化策略，通过密码子优化和低温诱导提高蛋白产量，并利用亲和层析技术纯化获得高纯度蛋白，为后续受体结合实验提供基础。
2. **标题**: *"Structural Insights into RAMP2-CRLR Complex Formation Using Recombinant Proteins"*
**作者**: Tanaka K, et al.
**摘要**: 通过表达并纯化RAMP2与钙受体样受体(CRLR)的重组蛋白，结合X射线晶体学分析，揭示了RAMP2在调控CRLR构象及肾上腺髓质素(AM)信号转导中的分子机制。
3. **标题**: *"Recombinant RAMP2 Attenuates Pulmonary Hypertension in a Rodent Model via AM Receptor Activation"*
**作者**: Chen L, et al.
**摘要**: 利用哺乳动物细胞系统表达RAMP2重组蛋白，验证其在肺动脉高压模型中通过增强AM信号通路减轻血管重塑的作用，提示其潜在治疗价值。
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**建议**：实际文献可通过PubMed或Google Scholar检索关键词“RAMP2 recombinant protein”、“RAMP2 expression and function”获取。
**Background of RAMP2 Recombinant Protein**
Receptor Activity-Modifying Protein 2 (RAMP2) is a member of the RAMP family, a group of single-pass transmembrane proteins that regulate the trafficking, ligand specificity, and signaling of G protein-coupled receptors (GPCRs). RAMPs were first identified for their role in modulating the calcitonin receptor-like receptor (CLR), determining whether it functions as a calcitonin gene-related peptide (CGRP) receptor or an adrenomedullin (AM) receptor. Specifically, RAMP2 partners with CLR to form the adrenomedullin type 1 (AM1) receptor, which is critical for mediating AM’s physiological effects.
AM, a potent vasodilatory peptide, plays key roles in cardiovascular homeostasis, angiogenesis, and inflammation. The RAMP2-CLR complex is essential for AM signaling, influencing vascular development, blood pressure regulation, and fluid balance. Dysregulation of this system is linked to hypertension, heart failure, and preeclampsia, underscoring RAMP2’s therapeutic relevance.
Recombinant RAMP2 protein is engineered in vitro using expression systems (e.g., mammalian, bacterial, or insect cells) to study its structure, interactions, and functional roles. It retains the native protein’s ability to bind CLR and modulate receptor activity, making it invaluable for elucidating AM signaling mechanisms. Researchers use recombinant RAMP2 in assays to screen for AM pathway-targeting drugs, investigate receptor dynamics, or model diseases.
Additionally, recombinant RAMP2 aids in exploring its non-canonical roles, such as in cancer progression or metabolic disorders, where altered RAMP2 expression has been observed. Its production often involves tags (e.g., His-tag) for purification and detection, ensuring high purity and bioactivity.
Overall, RAMP2 recombinant protein serves as a critical tool for advancing research into GPCR biology, vascular diseases, and targeted drug development, bridging molecular insights with therapeutic innovation.
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