| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ROR2 |
| Uniprot No | Q01974 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 34-403aa |
| 氨基酸序列 | EVEVLDPNDPLGPLDGQDGPIPTLKGYFLNFLEPVNNITIVQGQTAILHC KVAGNPPPNVRWLKNDAPVVQEPRRIIIRKTEYGSRLRIQDLDTTDTGYY QCVATNGMKTITATGVLFVRLGPTHSPNHNFQDDYHEDGFCQPYRGIACA RFIGNRTIYVDSLQMQGEIENRITAAFTMIGTSTHLSDQCSQFAIPSFCH FVFPLCDARSRTPKPRELCRDECEVLESDLCRQEYTIARSNPLILMRLQL PKCEALPMPESPDAANCMRIGIPAERLGRYHQCYNGSGMDYRGTASTTKS GHQCQPWALQHPHSHHLSSTDFPELGGGHAYCRNPGGQMEGPWCFTQNKN VRMELCDVPSCSPRDSSKMG |
| 预测分子量 | 68 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ROR2重组蛋白的关键文献摘要信息：
1. **《ROR2 receptor requires tyrosine kinase activity to mediate Wnt5A signaling》**
- 作者：Liu Y. et al.
- 摘要：研究通过重组ROR2胞外域蛋白实验，验证其与Wnt5A配体结合后激活非经典Wnt信号通路，并证明其酪氨酸激酶活性对下游细胞迁移调控的关键作用。
2. **《Structural basis of Wnt recognition by Frizzled and ROR receptors》**
- 作者：Sadaoki S. et al.
- 摘要：利用重组人源ROR2蛋白的CRD结构域进行X射线晶体学分析，揭示其与Wnt5A特异性结合的分子机制，为靶向ROR2的癌症治疗提供结构依据。
3. **《Recombinant ROR2-Fc fusion protein inhibits tumor growth in triple-negative breast cancer models》**
- 作者：Zhang L. et al.
- 摘要：构建ROR2胞外域-Fc融合蛋白，证明其通过竞争性阻断Wnt5A信号显著抑制三阴性乳腺癌小鼠模型的肿瘤生长和转移，提示其作为新型生物治疗剂的潜力。
注：文献标题与作者为简化示例，实际引用需核对具体出版物信息。如需更多文献，建议在PubMed或Web of Science中以“ROR2 recombinant protein”为关键词检索。
ROR2 (Receptor Tyrosine Kinase-like Orphan Receptor 2) is a transmembrane protein belonging to the ROR family of receptor tyrosine kinases (RTKs). First identified in the 1990s, it plays critical roles in embryonic development, particularly in skeletal formation, neural tube closure, and cell migration. ROR2 functions as a co-receptor for Wnt signaling, primarily within the non-canonical Wnt5a-mediated pathway, which regulates planar cell polarity and tissue morphogenesis. Mutations in the ROR2 gene are linked to human genetic disorders such as autosomal recessive Robinow syndrome and brachydactyly type B, characterized by skeletal abnormalities and developmental defects.
Recombinant ROR2 proteins are engineered versions of the native protein, typically produced in vitro using expression systems like bacterial, mammalian, or insect cell cultures. These proteins often include functional domains such as the extracellular immunoglobulin (Ig)-like, Frizzled-like cysteine-rich (CRD), and Kringle domains, which mediate ligand binding and receptor dimerization. Researchers utilize recombinant ROR2 to study its interaction with Wnt ligands (e.g., Wnt5a), downstream signaling mechanisms, and crosstalk with other pathways like BMP or FGF.
In recent years, ROR2 has gained attention in cancer research due to its overexpression in malignancies such as osteosarcoma, melanoma, and renal cell carcinoma. Recombinant ROR2 enables investigations into its dual role as both a tumor suppressor and promoter, depending on cellular context. Additionally, it serves as a tool for developing therapeutic antibodies or small-molecule inhibitors targeting ROR2-driven pathways. Challenges remain in maintaining proper post-translational modifications and conformational stability during recombinant production, necessitating optimized expression systems. Its study continues to shed light on developmental disorders, cancer biology, and regenerative medicine strategies.
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