| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | THBS2 |
| Uniprot No | P35442 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1172aa |
| 氨基酸序列 | MVWRLVLLALWVWPSTQAGHQDKDTTFDLFSISNINRKTIGAKQFRGPDPGVPAYRFVRFDYIPPVNADDLSKITKIMRQKEGFFLTAQLKQDGKSRGTLLALEGPGLSQRQFEIVSNGPADTLDLTYWIDGTRHVVSLEDVGLADSQWKNVTVQVAGETYSLHVGCDLIDSFALDEPFYEHLQAEKSRMYVAKGSARESHFRGLLQNVHLVFENSVEDILSKKGCQQGQGAEINAISENTETLRLGPHVTTEYVGPSSERRPEVCERSCEELGNMVQELSGLHVLVNQLSENLKRVSNDNQFLWELIGGPPKTRNMSACWQDGRFFAENETWVVDSCTTCTCKKFKTICHQITCPPATCASPSFVEGECCPSCLHSVDGEEGWSPWAEWTQCSVTCGSGTQQRGRSCDVTSNTCLGPSIQTRACSLSKCDTRIRQDGGWSHWSPWSSCSVTCGVGNITRIRLCNSPVPQMGGKNCKGSGRETKACQGAPCPIDGRWSPWSPWSACTVTCAGGIRERTRVCNSPEPQYGGKACVGDVQERQMCNKRSCPVDGCLSNPCFPGAQCSSFPDGSWSCGSCPVGFLGNGTHCEDLDECALVPDICFSTSKVPRCVNTQPGFHCLPCPPRYRGNQPVGVGLEAAKTEKQVCEPENPCKDKTHNCHKHAECIYLGHFSDPMYKCECQTGYAGDGLICGEDSDLDGWPNLNLVCATNATYHCIKDNCPHLPNSGQEDFDKDGIGDACDDDDDNDGVTDEKDNCQLLFNPRQADYDKDEVGDRCDNCPYVHNPAQIDTDNNGEGDACSVDIDGDDVFNERDNCPYVYNTDQRDTDGDGVGDHCDNCPLVHNPDQTDVDNDLVGDQCDNNEDIDDDGHQNNQDNCPYISNANQADHDRDGQGDACDPDDDNDGVPDDRDNCRLVFNPDQEDLDGDGRGDICKDDFDNDNIPDIDDVCPENNAISETDFRNFQMVPLDPKGTTQIDPNWVIRHQGKELVQTANSDPGIAVGFDEFGSVDFSGTFYVNTDRDDDYAGFVFGYQSSSRFYVVMWKQVTQTYWEDQPTRAYGYSGVSLKVVNSTTGTGEHLRNALWHTGNTPGQVRTLWHDPRNIGWKDYTAYRWHLTHRPKTGYIRVLVHEGKQVMADSGPIYDQTYAGGRLGLFVFSQEMVYFSDLKYECRDI |
| 预测分子量 | 129,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于THBS2重组蛋白的3篇参考文献及其简要摘要：
1. **《Recombinant THBS2 suppresses tumor growth by modulating angiogenesis and immune response in a murine model》**
- **作者**: Li X, et al.
- **摘要**: 研究利用重组THBS2蛋白在小鼠肿瘤模型中验证其通过抑制血管生成和增强抗肿瘤免疫反应(如调节T细胞浸润)抑制肿瘤生长的作用，提示其潜在治疗应用。
2. **《Structural and functional analysis of recombinant THBS2 in extracellular matrix remodeling》**
- **作者**: Smith JR, et al.
- **摘要**: 通过重组THBS2蛋白解析其三维结构，发现其通过结合整合素和基质金属蛋白酶(MMPs)调控细胞外基质重塑，影响细胞迁移和组织修复过程。
3. **《THBS2 as a diagnostic biomarker: Serum levels correlate with fibrosis progression in chronic liver disease》**
- **作者**: Wang Y, et al.
- **摘要**: 研究表明重组THBS2蛋白可作为定量检测工具，发现慢性肝病患者血清THBS2水平与肝纤维化程度正相关，提示其作为无创诊断标志物的潜力。
4. **《Recombinant THBS2 inhibits TGF-β signaling and attenuates renal fibrosis in vitro》**
- **作者**: Kim H, et al.
- **摘要**: 体外实验显示重组THBS2蛋白通过阻断TGF-β/Smad通路减少肾成纤维细胞活化，为靶向THBS2治疗纤维化疾病提供机制依据。
*注：以上文献为虚拟示例，实际研究需查阅具体数据库(如PubMed)。建议通过关键词“THBS2 recombinant protein”或“THBS2 function”检索近期论文。*
**Background of THBS2 Recombinant Protein**
Thrombospondin-2 (THBS2) is a multifunctional extracellular matrix (ECM) glycoprotein belonging to the thrombospondin family, which plays critical roles in cell adhesion, migration, proliferation, and tissue remodeling. Encoded by the *THBS2* gene, this secreted protein is structurally characterized by conserved domains, including N-terminal, procollagen, type 1 repeats, type 2 repeats, and a C-terminal domain, enabling interactions with various ECM components, cell surface receptors (e.g., CD36. integrins), and growth factors. Unlike its homolog THBS1. THBS2 is preferentially expressed during tissue development and repair, influencing angiogenesis, inflammation, and matrix organization.
Recombinant THBS2 protein is produced using biotechnological platforms, such as mammalian or prokaryotic expression systems, to ensure proper folding and post-translational modifications. Purification techniques like affinity chromatography yield high-purity, bioactive THBS2. which retains its native functional properties. This recombinant form is widely used in research to study its dual role in diseases. For instance, THBS2 exhibits context-dependent effects in cancer: it can suppress tumor growth by inhibiting angiogenesis or promote metastasis via ECM modulation. Elevated THBS2 levels are linked to fibrosis, cardiovascular diseases, and osteoarthritis, highlighting its diagnostic or therapeutic potential.
In recent years, THBS2 has gained attention as a biomarker for certain cancers and fibrotic disorders. Studies also explore its utility in regenerative medicine, given its ability to regulate stem cell behavior and tissue repair. However, its complex interactions and signaling mechanisms remain under investigation, necessitating further research to harness its full clinical potential.
Overall, recombinant THBS2 serves as a vital tool for deciphering the protein’s biological functions and developing targeted therapies for ECM-related pathologies.
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