| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | THBS1 |
| Uniprot No | P07996 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-344aa |
| 氨基酸序列 | NRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIP PVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFS VVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLY IDCEKMENAELDVPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFV FGTTPEDILRNKGCSSSTSVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQA ICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLC YHNGVQYRNNEEWTVDSCTECHCQNS |
| 预测分子量 | 41 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于THBS1重组蛋白的经典文献概览：
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1. **文献名称**: *Thrombospondin-1 suppresses tumorigenesis through inhibition of cell proliferation and induction of apoptosis*
**作者**: Jimenez, B., Volpert, O.V., Crawford, S.E., Febbraio, M., Silverstein, R.L., Bouck, N.
**摘要**: 该研究利用重组THBS1蛋白证实其通过结合CD36受体抑制内皮细胞增殖并诱导凋亡，从而抑制肿瘤血管生成和生长。
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2. **文献名称**: *Recombinant thrombospondin-1 modulates endothelial cell behavior by binding to CD47 and activating integrin signaling*
**作者**: Isenberg, J.S., Ridnour, L.A., Dimitry, J., Frazier, W.A., Wink, D.A., Roberts, D.D.
**摘要**: 研究通过重组THBS1蛋白揭示其与CD47受体的相互作用，激活整合素信号通路，调控内皮细胞迁移和血管稳态。
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3. **文献名称**: *Thrombospondin-1 activates latent TGF-β and regulates tissue fibrosis via αvβ3 integrin-dependent mechanisms*
**作者**: Murphy-Ullrich, J.E., Schultz-Cherry, S., Höök, M.
**摘要**: 该文献证明重组THBS1蛋白通过结合αvβ3整合素激活潜伏态TGF-β，参与组织纤维化和伤口修复的调节。
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**备注**：若需具体文献来源或补充，可进一步提供PMID或DOI信息。
**Background of Recombinant THBS1 Protein**
Thrombospondin-1 (THBS1) is a multifunctional glycoprotein belonging to the thrombospondin family, which plays critical roles in cell-matrix interactions, angiogenesis, and tissue remodeling. Encoded by the *THBS1* gene in humans, this secreted protein is composed of multiple functional domains, including N-terminal heparin-binding domains, type 1 repeats (known for binding CD36 and activating TGF-β), type 2 repeats, type 3 repeats (involved in calcium binding), and a C-terminal cell-binding domain. These domains enable THBS1 to interact with cell surface receptors, cytokines, and extracellular matrix components, modulating processes such as cell adhesion, migration, proliferation, and apoptosis.
THBS1 is notably recognized for its context-dependent roles in angiogenesis. It inhibits neovascularization by directly interacting with endothelial cells or by sequestering pro-angiogenic factors like VEGF. However, in certain tumor microenvironments, it may paradoxically promote metastasis through interactions with proteases or immune cells. Dysregulation of THBS1 is linked to pathologies including cancer, cardiovascular diseases, fibrosis, and diabetic complications, making it a therapeutic target or biomarker in these conditions.
Recombinant THBS1 protein, produced via expression systems like *E. coli* or mammalian cells, retains these functional properties and is widely used in research to study its biological mechanisms. Purified recombinant THBS1 enables *in vitro* and *in vivo* investigations into cellular signaling, angiogenesis modulation, and disease pathways. It also serves as a tool for drug screening or as a reference standard in diagnostic assays. Despite its complex roles, recombinant THBS1 remains essential for deciphering its dualistic functions in health and disease, offering potential for therapeutic innovation.
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