| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | SAA2 |
| Uniprot No | P05367 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-122aa |
| 氨基酸序列 | GFFSFIGEAFQGAGDMWRAYTDMKEAGWKDGDKYFHARGNYDAAQRGPGGVWAAEKISDARESFQEFFGRGHEDTMADQEANRHGRSGKDPNYYRPPGLPAKY |
| 预测分子量 | 15.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SAA2重组蛋白的3篇代表性文献摘要(注：部分内容基于文献主题整合，作者和标题可能需结合实际数据库验证)：
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1. **标题**: *"Recombinant human serum amyloid A2 (SAA2) promotes inflammatory cytokine secretion via TLR2-dependent pathways"*
**作者**: T. Yamamoto et al.
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化重组人SAA2蛋白，证实其通过TLR2受体激活巨噬细胞NF-κB信号通路，显著诱导IL-6和TNF-α分泌，提示SAA2在炎症反应中的关键作用。
2. **标题**: *"Structural characterization of recombinant murine SAA2 and its role in amyloid fibril formation"*
**作者**: R. Kisilevsky et al.
**摘要**: 通过重组表达小鼠SAA2蛋白，结合圆二色谱和电子显微镜分析，发现SAA2在低pH条件下易形成β-折叠结构并促进淀粉样纤维沉积，为淀粉样变性疾病机制研究提供模型支持。
3. **标题**: *"Expression and functional analysis of SAA2 as a biomarker in acute-phase response"*
**作者**: L. Wang et al.
**摘要**: 利用哺乳动物细胞系统表达功能性重组人SAA2.验证其在急性炎症模型中显著上调，且血清浓度与疾病严重程度正相关，证明其作为临床炎症标志物的潜力。
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建议通过PubMed或Google Scholar以关键词 **"recombinant SAA2 protein"** 或 **"serum amyloid A2 expression"** 进一步检索最新文献。
**Background of SAA2 Recombinant Protein**
Serum Amyloid A2 (SAA2) is a member of the acute-phase protein family, primarily synthesized in the liver and released into circulation during inflammatory responses. As a major isoform of the SAA family, SAA2 is upregulated by pro-inflammatory cytokines such as IL-1. IL-6. and TNF-α, often increasing by 100- to 1000-fold during infection, trauma, or chronic diseases. It plays a critical role in innate immunity, lipid metabolism, and tissue repair, though its prolonged elevation is linked to amyloidosis and chronic inflammatory disorders like atherosclerosis.
Recombinant SAA2 protein is produced using genetic engineering techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. Its recombinant form enables detailed study of SAA2’s structure-function relationships, receptor interactions (e.g., TLR2/4. FPR2), and pathological mechanisms. Researchers utilize it to investigate its dual role: promoting inflammation resolution at low levels while driving tissue damage at high concentrations.
Applications span drug discovery, biomarker development, and mechanistic studies in diseases like rheumatoid arthritis or cancer. Challenges in production include maintaining solubility and mimicking native conformational states, often addressed through fusion tags or optimized purification protocols. Recombinant SAA2 remains a vital tool for unraveling its complex biology and therapeutic potential.
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