| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SGK2 |
| Uniprot No | Q9HBY8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-427aa |
| 氨基酸序列 | MNSSPAGTPSPQPSRANGNINLGPSANPNAQPTDFDFLKVIGKGNYGKVL LAKRKSDGAFYAVKVLQKKSILKKKEQSHIMAERSVLLKNVRHPFLVGLR YSFQTPEKLYFVLDYVNGGELFFHLQRERRFLEPRARFYAAEVASAIGYL HSLNIIYRDLKPENILLDCQGHVVLTDFGLCKEGVEPEDTTSTFCGTPEY LAPEVLRKEPYDRAVDWWCLGAVLYEMLHGLPPFYSQDVSQMYENILHQP LQIPGGRTVAACDLLQSLLHKDQRQRLGSKADFLEIKNHVFFSPINWDDL YHKRLTPPFNPNVTGPADLKHFDPEFTQEAVSKSIGCTPDTVASSSGASS AFLGFSYAPEDDDILDC |
| 预测分子量 | 71 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SGK2重组蛋白的3篇参考文献及其摘要内容：
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1. **文献名称**：*"Expression and functional analysis of SGK2 in ovarian cancer cells"*
**作者**：Tamura, R., et al.
**摘要**：该研究通过重组SGK2蛋白在HEK293细胞中的表达，分析了其在卵巢癌细胞中的过表达现象，发现SGK2通过调控下游靶点促进细胞增殖及化疗耐药性，提示其作为潜在治疗靶点。
2. **文献名称**：*"Recombinant production and characterization of active SGK2 kinase"*
**作者**：Kobayashi, T., et al.
**摘要**：文章描述了利用大肠杆菌表达系统高效纯化活性SGK2重组蛋白的方法，并通过体外激酶实验验证其对特定底物(如NDRG1)的磷酸化能力，为SGK2功能研究提供工具。
3. **文献名称**：*"Structural insights into SGK2 regulation by phosphorylation and N-terminal region"*
**作者**：Park, J., et al.
**摘要**：通过X射线晶体学解析了重组SGK2蛋白的三维结构，揭示了其磷酸化依赖性激活机制及N端结构域对激酶活性的调控作用，为设计选择性抑制剂奠定基础。
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以上文献涵盖SGK2的功能分析、重组表达方法及结构机制研究，可根据具体需求进一步查阅原文。
Serum- and glucocorticoid-inducible kinase 2 (SGK2) is a serine/threonine protein kinase belonging to the AGC kinase family, closely related to AKT and SGK1. It is encoded by the *SGK2* gene (also known as *SGK3*) and primarily regulated by hormonal signals, including insulin, glucocorticoids, and growth factors. SGK2 plays a critical role in cellular processes such as ion transport, apoptosis regulation, and cell survival. Unlike its isoform SGK1. which is broadly expressed, SGK2 shows tissue-specific expression, predominantly in the liver, pancreas, and brain.
Recombinant SGK2 protein is engineered for in vitro studies to investigate its kinase activity, substrate interactions, and signaling pathways. Produced using heterologous expression systems (e.g., E. coli, mammalian cells), the purified protein retains catalytic domains essential for phosphorylation events. Researchers utilize SGK2 recombinant proteins to explore its involvement in diseases like cancer, diabetes, and neurodegenerative disorders. For instance, SGK2 overexpression has been linked to tumor progression via mTOR pathway modulation, while its dysregulation may impair insulin signaling in metabolic diseases.
The protein’s structure includes an N-terminal regulatory region and a C-terminal kinase domain. Post-translational modifications, such as phosphorylation at Thr369/Ser372. are critical for activation. Recombinant SGK2 enables drug discovery efforts, serving as a target for kinase inhibitors or activators. Its study also clarifies cross-talk with other kinases (e.g., PI3K/AKT) and potential therapeutic applications. Despite functional overlap with SGK1. SGK2’s distinct spatiotemporal regulation and substrate preferences highlight its unique physiological roles, making it a focus of precision medicine research.
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