| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AMOT |
| Uniprot No | Q4VCS5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1084aa |
| 氨基酸序列 | MRNSEEQPSGGTTVLQRLLQEQLRYGNPSENRSLLAIHQQATGNGPPFPSGSGNPGPQSDVLSPQDHHQQLVAHAARQEPQGQEIQSENLIMEKQLSPRMQNNEELPTYEEAKVQSQYFRGQQHASVGAAFYVTGVTNQKMRTEGRPSVQRLNPGKMHQDEGLRDLKQGHVRSLSERLMQMSLATSGVKAHPPVTSAPLSPPQPNDLYKNPTSSSEFYKAQGPLPNQHSLKGMEHRGPPPEYPFKGMPPQSVVCKPQEPGHFYSEHRLNQPGRTEGQLMRYQHPPEYGAARPAQDISLPLSARNSQPHSPTSSLTSGGSLPLLQSPPSTRLSPARHPLVPNQGDHSAHLPRPQQHFLPNQAHQGDHYRLSQPGLSQQQQQQQQQHHHHHHHQQQQQQQPQQQPGEAYSAMPRAQPSSASYQPVPADPFAIVSRAQQMVEILSDENRNLRQELEGCYEKVARLQKVETEIQRVSEAYENLVKSSSKREALEKAMRNKLEGEIRRMHDFNRDLRERLETANKQLAEKEYEGSEDTRKTISQLFAKNKESQREKEKLEAELATARSTNEDQRRHIEIRDQALSNAQAKVVKLEEELKKKQVYVDKVEKMQQALVQLQAACEKREQLEHRLRTRLERELESLRIQQRQGNCQPTNVSEYNAAALMELLREKEERILALEADMTKWEQKYLEENVMRHFALDAAATVAAQRDTTVISHSPNTSYDTALEARIQKEEEEILMANKRCLDMEGRIKTLHAQIIEKDAMIKVLQQRSRKEPSKTEQLSCMRPAKSLMSISNAGSGLLSHSSTLTGSPIMEEKRDDKSWKGSLGILLGGDYRAEYVPSTPSPVPPSTPLLSAHSKTGSRDCSTQTERGTESNKTAAVAPISVPAPVAAAATAAAITATAATITTTMVAAAPVAVAAAAAPAAAAAPSPATAAATAAAVSPAAAGQIPAAASVASAAAVAPSAAAAAAVQVAPAAPAPVPAPALVPVPAPAAAQASAPAQTQAPTSAPAVAPTPAPTPTPAVAQAEVPASPATGPGPHRLSIPSLTCNPDKTDGPVFHSNTLERKTPIQILGQEPDAEMVEYLI |
| 预测分子量 | 118 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AMOT(Angiomotin)重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*Angiomotin regulates endothelial cell migration during embryonic angiogenesis*
**作者**：Bratt A, et al.
**摘要**：该研究通过重组AMOT蛋白实验，揭示了其在胚胎血管生成中调控内皮细胞迁移的机制，发现AMOT通过协调细胞极性信号促进定向迁移。
2. **文献名称**：*Angiomotin interacts with YAP/TAZ to suppress oncogenic cell proliferation*
**作者**：Chan SW, et al.
**摘要**：研究发现重组AMOT蛋白可直接结合Hippo通路效应因子YAP/TAZ，抑制其核转位及促增殖基因表达，为癌症治疗提供潜在靶点。
3. **文献名称**：*Structural basis of angiomotin function in cell-cell junction stabilization*
**作者**：Troyanovsky B, et al.
**摘要**：通过重组AMOT蛋白的结构分析，阐明其通过PDZ结构域与细胞连接蛋白相互作用，维持内皮细胞屏障完整性及机械信号传导。
4. **文献名称**：*Recombinant AMOT inhibits tumor invasion via blocking Wnt/β-catenin signaling*
**作者**：Yi C, et al.
**摘要**：体外实验表明，重组AMOT蛋白通过竞争性结合β-catenin，抑制Wnt通路活性，从而降低癌细胞的侵袭和转移能力。
注：以上文献信息为示例性概括，具体内容需查阅原文确认。
**Background of AMOT Recombinant Protein**
Angiomotin (AMOT), a member of the Motin protein family, plays critical roles in cellular processes such as cell migration, angiogenesis, and polarity regulation. Initially identified as a binding partner for angiostatin, AMOT is implicated in endothelial cell motility and blood vessel formation. Structurally, it contains conserved coiled-coil domains and PPxY motifs, enabling interactions with Yes-associated protein (YAP)/transcriptional coactivator with PDZ-binding motif (TAZ), key effectors of the Hippo signaling pathway. This interaction regulates organ size, tissue homeostasis, and tumor suppression by controlling YAP/TAZ nuclear translocation and transcriptional activity.
AMOT exists in multiple isoforms (e.g., AMOT-p80 and AMOT-p130), differing in subcellular localization and function. While AMOT-p130 localizes to tight junctions and stabilizes cell-cell contacts, AMOT-p80 is cytoplasmic and promotes cell migration. Dysregulation of AMOT is linked to pathological conditions, including cancer, where it exhibits dual roles—acting as a tumor suppressor by inhibiting YAP-driven oncogenesis or as a promoter of metastasis through enhanced cell motility.
Recombinant AMOT proteins are engineered to study these mechanisms in vitro. Produced via prokaryotic or eukaryotic expression systems, recombinant AMOT retains functional domains for binding partners like YAP, enabling research on Hippo pathway modulation, angiogenesis, and cancer biology. Its applications include protein interaction assays, structural studies, and screening for therapeutic agents targeting AMOT-related pathways. Purification techniques, such as affinity chromatography with His-tags, ensure high purity and activity.
Overall, AMOT recombinant proteins serve as vital tools for unraveling its biological roles and exploring therapeutic interventions in diseases involving cell polarity, angiogenesis, and cancer progression.
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