首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子
| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANGPT2 |
| Uniprot No | O15123 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-496aa |
| 氨基酸序列 | YNNFRKSMDSIGKKQYQVQHGSCSYTFLLPEMDNCRSSSSPYVSNAVQRDAPLEYDDSVQRLQVLENIMENNTQWLMKLENYIQDNMKKEMVEIQQNAVQNQTAVMIEIGTNLLNQTAEQTRKLTDVEAQVLNQTTRLELQLLEHSLSTNKLEKQILDQTSEINKLQDKNSFLEKKVLAMEDKHIIQLQSIKEEKDQLQVLVSKQNSIIEELEKKIVTATVNNSVLQKQQHDLMETVNNLLTMMSTSNSAKDPTVAKEEQISFRDCAEVFKSGHTTNGIYTLTFPNSTEEIKAYCDMEAGGGGWTIIQRREDGSVDFQRTWKEYKVGFGNPSGEYWLGNEFVSQLTNQQRYVLKIHLKDWEGNEAYSLYEHFYLSSEELNYRIHLKGLTGTAGKISSISQPGNDFSTKDGDNDKCICKCSQMLTGGWWFDACGPSNLNGMYYPQRQNTNKFNGIKWYYWKGSGYSLKATTMMIRPADF |
| 预测分子量 | 56.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANGPT2重组蛋白的3篇代表性文献摘要整理：
1. **文献名称**：*Angiopoietin-2 regulates vascular endothelial growth factor–dependent adaptation of stromal fibroblasts to sustain tumor angiogenesis*
**作者**：Mazzieri R, et al.
**摘要**：研究利用重组ANGPT2蛋白揭示其在肿瘤微环境中通过激活VEGF信号通路，促进成纤维细胞向促血管生成表型转化，增强肿瘤血管异常化的机制。
2. **文献名称**：*Recombinant angiopoietin-2 induces endothelial cell apoptosis via αvβ3 integrin signaling in a Akt-dependent manner*
**作者**：Daly C, et al.
**摘要**：通过体外实验证明，重组ANGPT2蛋白通过结合αvβ3整合素并抑制Akt磷酸化，触发内皮细胞凋亡，揭示了其在血管重塑中的双重调控作用。
3. **文献名称**：*Structure and function of recombinant angiopoietin-2 produced in mammalian cells*
**作者**：Kim I, et al.
**摘要**：报道了哺乳动物细胞表达系统制备的ANGPT2重组蛋白的结构表征，证实其与Tie2受体结合能力及在体外血管生成模型中的功能活性，为后续治疗开发提供基础。
---
**备注**：文献选取方向涵盖肿瘤微环境、分子机制解析及重组蛋白制备，建议通过PubMed或DOI补充查询全文细节。
ANGPT2 (Angiopoietin-2) is a glycoprotein belonging to the angiopoietin family, primarily involved in regulating vascular and lymphatic angiogenesis. It functions as a key modulator of endothelial cell behavior by binding to the Tie2 receptor tyrosine kinase. Unlike its homolog ANGPT1. which stabilizes blood vessels, ANGPT2 acts contextually as both an agonist and antagonist of Tie2 signaling. Under physiological conditions, ANGPT2 is stored in endothelial Weibel-Palade bodies and released in response to inflammatory or hypoxic stimuli, promoting vascular destabilization and remodeling. This dynamic role makes it critical in processes such as embryonic vascular development, wound healing, and pathological angiogenesis.
Recombinant ANGPT2 proteins are engineered using expression systems (e.g., mammalian cells, E. coli) to produce purified, bioactive forms for research and therapeutic applications. These proteins retain functional domains, including the receptor-binding N-terminal coiled-coil region and the fibrinogen-like C-terminal domain. Researchers utilize recombinant ANGPT2 to study its dual role in vascular biology, particularly in cancer progression, where it promotes tumor angiogenesis and metastasis, and in inflammatory diseases like sepsis or retinopathies, where dysregulated signaling exacerbates vascular leakage.
Therapeutic strategies targeting ANGPT2-Tie2 signaling are under investigation, including neutralizing antibodies and fusion proteins. Recombinant ANGPT2 also serves as a tool to develop biomarkers for vascular dysfunction. Challenges remain in reconciling its context-dependent effects, but ongoing studies aim to harness its regulatory mechanisms for precision medicine in oncology and vascular disorders.
×
