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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANG |
| Uniprot No | P03950 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-147aa |
| 氨基酸序列 | DNSRYTHFLTQHYDAKPQGRDDRYCESIMRRRGLTSPCKDINTFIHGNKRSIKAICENKNGNPHRENLRISKSSFQVTTCKLHGGSPWPPCQYRATAGFRNVVVACENGLPVHLDQSIFRRP |
| 预测分子量 | 18.0kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组ANG(Angiogenin)蛋白的3篇参考文献，信息基于真实研究领域，但具体作者和标题为虚拟示例：
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1. **标题**: "Expression and Purification of Recombinant Human Angiogenin in Escherichia coli"
**作者**: Smith J, et al.
**摘要**: 本研究报道了利用大肠杆菌表达系统高效生产重组人ANG蛋白的优化方法。通过密码子优化和诱导条件调控，成功获得可溶性蛋白，并经离子交换层析纯化。活性检测表明重组ANG具有促进内皮细胞迁移的生物学功能，为后续功能研究奠定基础。
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2. **标题**: "Angiogenin's Role in ALS: Neuroprotective Effects of Recombinant Protein in Motor Neurons"
**作者**: Lee H, et al.
**摘要**: 文章探讨重组ANG蛋白在肌萎缩侧索硬化(ALS)模型中的神经保护作用。体外实验显示，重组ANG能抑制运动神经元凋亡并促进RNA稳定性，机制可能与调控应激颗粒形成相关，提示其在神经退行性疾病治疗中的潜力。
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3. **标题**: "Structural Insights into the RNA-Binding Activity of Recombinant Angiogenin by X-ray Crystallography"
**作者**: García R, et al.
**摘要**: 通过X射线晶体学解析重组ANG蛋白与RNA底物的复合物结构，揭示了其核糖核酸酶活性位点及RNA结合域的关键残基。该研究阐明了ANG在基因表达调控中的分子机制，为设计靶向ANG的药物提供结构基础。
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如需具体文献，建议通过PubMed或Web of Science检索关键词“recombinant angiogenin”获取最新研究。
**Background of ANG Recombinant Protein**
Angiogenin (ANG), a member of the ribonuclease (RNase) A superfamily, is a multifunctional protein initially identified for its role in promoting blood vessel formation (angiogenesis). Unlike classical RNases, ANG exhibits relatively weak ribonucleolytic activity but plays critical roles in cell survival, proliferation, and stress response. It is expressed in various tissues and is particularly vital in physiological processes such as embryogenesis, wound healing, and tumor progression. ANG’s unique ability to cleave tRNA under stress conditions generates tiRNAs (tRNA-derived stress-induced RNAs), which modulate translation and enhance cell adaptation to environmental stressors.
Recombinant ANG protein is produced using biotechnological methods, typically through expression in *E. coli*, yeast, or mammalian cell systems. This allows large-scale production of purified, bioactive ANG for research and therapeutic applications. Recombinant ANG retains key functional properties, including ribonuclease activity, nuclear translocation capacity, and interactions with cellular receptors like nucleolin.
Research highlights ANG’s dual roles: it supports cell survival under mild stress but may trigger apoptosis under severe conditions. Dysregulation of ANG is linked to cancers, neurodegenerative diseases (e.g., ALS), and ischemic conditions. In cancer, ANG often exhibits elevated expression, promoting tumor angiogenesis and growth. Conversely, in neurodegenerative contexts, ANG mutations or deficiencies impair RNA homeostasis, contributing to neuronal death.
Therapeutic exploration of recombinant ANG focuses on its neuroprotective and angiogenic potential. For instance, it is being studied for treating stroke, ALS, and chronic wounds. Challenges include understanding its context-dependent mechanisms, optimizing delivery systems, and ensuring targeted activity. Overall, recombinant ANG serves as a valuable tool for deciphering its biological complexity and developing novel therapies.
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