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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ANGPTL6 |
| Uniprot No | Q8NI99 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-470aa |
| 氨基酸序列 | RAGAPRCTYTFVLPPQKFTGAVCWSGPASTRATPEAANASELAALRMRVGRHEELLRELQRLAAADGAVAGEVRALRKESRGLSARLGQLRAQLQHEAGPGAGPGADLGAEPAAALALLGERVLNASAEAQRAAARFHQLDVKFRELAQLVTQQSSLIARLERLCPGGAGGQQQVLPPPPLVPVVPVRLVGSTSDTSRMLDPAPEPQRDQTQRQQEPMASPMPAGHPAVPTKPVGPWQDCAEARQAGHEQSGVYELRVGRHVVSVWCEQQLEGGGWTVIQRRQDGSVNFFTTWQHYKAGFGRPDGEYWLGLEPVYQLTSRGDHELLVLLEDWGGRGARAHYDGFSLEPESDHYRLRLGQYHGDAGDSLSWHNDKPFSTVDRDRDSYSGNCALYQRGGWWYHACAHSNLNGVWHHGGHYRSRYQDGVYWAEFRGGAYSLRKAAMLIRPLKL |
| 预测分子量 | 56.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ANGPTL6重组蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**：*ANGPTL6 promotes insulin sensitivity and glucose uptake by activating the PI3K/AKT signaling pathway in skeletal muscle*
**作者**：Chen Y, et al.
**摘要**：该研究通过重组ANGPTL6蛋白处理小鼠骨骼肌细胞，发现其通过激活PI3K/AKT信号通路增强胰岛素敏感性，并促进葡萄糖摄取，提示其在2型糖尿病治疗中的潜在应用。
2. **文献名称**：*Structural and functional characterization of recombinant human ANGPTL6 reveals its role in angiogenesis and tumor growth*
**作者**：Kojima T, et al.
**摘要**：作者利用哺乳动物细胞系统表达重组人ANGPTL6蛋白，解析其结构特征，并证明其通过促进内皮细胞增殖和迁移加速血管生成，同时在肿瘤异种移植模型中观察到促癌生长作用。
3. **文献名称**：*ANGPTL6 regulates adipose tissue lipid metabolism through lipoprotein lipase activity modulation*
**作者**：Xu A, et al.
**摘要**：研究显示重组ANGPTL6蛋白通过抑制脂肪组织中脂蛋白脂肪酶(LPL)活性，调节脂质代谢平衡，高表达ANGPTL6的转基因小鼠表现出肥胖抵抗表型，提示其在代谢综合征中的调控机制。
注：以上文献信息为示例性内容，实际研究中请以具体发表的论文数据为准。建议通过PubMed或Web of Science以“ANGPTL6 recombinant protein”为关键词检索最新文献。
**Background of ANGPTL6 Recombinant Protein**
Angiopoietin-like protein 6 (ANGPTL6), also known as angiopoietin-related growth factor (AGF), is a secreted glycoprotein belonging to the angiopoietin-like family. It plays a multifaceted role in metabolic regulation, energy homeostasis, and tissue repair. Structurally, ANGPTL6 contains a coiled-coil N-terminal domain and a fibrinogen-like C-terminal domain, which are critical for its biological activity. Primarily expressed in the liver and adipose tissue, ANGPTL6 enhances insulin sensitivity, promotes glucose uptake, and modulates lipid metabolism by inhibiting lipoprotein lipase (LPL) activity, thereby influencing triglyceride clearance.
Studies highlight its protective role in metabolic disorders. Transgenic mice overexpressing ANGPTL6 exhibit resistance to diet-induced obesity, improved glucose tolerance, and reduced inflammation. Conversely, ANGPTL6 deficiency exacerbates metabolic dysfunction, underscoring its importance in maintaining metabolic health. Beyond metabolism, ANGPTL6 is implicated in angiogenesis and tissue regeneration, particularly in the heart and skeletal muscle, by stimulating endothelial cell proliferation and migration.
Recombinant ANGPTL6 protein is engineered using expression systems like *E. coli* or mammalian cells to ensure proper folding and post-translational modifications. This bioactive form enables researchers to study its mechanisms in vitro and in vivo, including its interaction with receptors such as integrins and its role in signaling pathways like PI3K/Akt and AMPK. Potential therapeutic applications are emerging, with ANGPTL6 explored as a candidate for treating obesity, type 2 diabetes, and cardiovascular diseases. However, its dual role in metabolism and angiogenesis necessitates careful evaluation to balance benefits and risks, such as unintended vascular proliferation.
In summary, ANGPTL6 recombinant protein serves as a vital tool for unraveling metabolic and regenerative pathways, offering promising avenues for translational research in metabolic syndromes and tissue repair.
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