Recombinant Human ALT2 protein

中文名： 谷丙转氨酶2(ALT2)重组蛋白
别名： ALT2；AAT2；ALT2；Alanine aminotransferase 2

货号: PA1000-8509

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	ALT2
Uniprot No	Q8TD30
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-523aa
氨基酸序列	MGSSHHHHHH SSGLVPRGSH MGSMQRAAAL VRRGCGPRTP SSWGRSQSSA AAEASAVLKV RPERSRRERI LTLESMNPQV KAVEYAVRGP IVLKAGEIEL ELQRGIKKPF TEVIRANIGD AQAMGQQPIT FLRQVMALCT YPNLLDSPSF PEDAKKRARR ILQACGGNSL GSYSASQGVN CIREDVAAYI TRRDGGVPAD PDNIYLTTGA SDGISTILKI LVSGGGKSRT GVMIPIPQYP LYSAVISELD AIQVNYYLDE ENCWALNVNE LRRAVQEAKD HCDPKVLCII NPGNPTGQVQ SRKCIEDVIH FAWEEKLFLL ADEVYQDNVY SPDCRFHSFK KVLYEMGPEY SSNVELASFH STSKGYMGEC GYRGGYMEVI NLHPEIKGQL VKLLSVRLCP PVSGQAAMDI VVNPPVAGEE SFEQFSREKE SVLGNLAKKA KLTEDLFNQV PGIHCNPLQG AMYAFPRIFI PAKAVEAAQA HQMAPDMFYC MKLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPVEKLKTV LQKVKDFHIN FLEKYA
预测分子量	60 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于ALT2重组蛋白的参考文献示例，涵盖不同研究方向：

1. **"Expression and Characterization of Recombinant Human ALT2 in E. coli"**

*作者：Zhang L, et al.*

**摘要**：研究成功在大肠杆菌中表达并纯化了人源ALT2重组蛋白，分析了其酶动力学特性，证实其催化丙氨酸与α-酮戊二酸转化的活性，并探讨了pH和温度对酶活性的影响。

2. **"Mitochondrial ALT2 Isoform: A Key Regulator of Glutamine Metabolism in Cancer Cells"**

*作者：Wang Y, et al.*

**摘要**：揭示了ALT2重组蛋白在肿瘤细胞线粒体中的功能，证明其通过调控谷氨酰胺代谢促进肿瘤增殖，为靶向ALT2的癌症治疗提供了理论依据。

3. **"Structural Insights into the Catalytic Mechanism of ALT2 via Crystallography"**

*作者：Kimura T, et al.*

**摘要**：通过X射线晶体学解析了重组ALT2蛋白的三维结构，阐明了其底物结合位点及催化机制，为设计特异性抑制剂奠定结构基础。

4. **"Comparative Analysis of ALT1 and ALT2 Recombinant Proteins in Liver Disease Models"**

*作者：Chen H, et al.*

**摘要**：对比ALT1与ALT2重组蛋白在肝损伤模型中的表达差异，发现ALT2特异性上调与线粒体功能障碍相关，提示其作为肝病生物标志物的潜力。

*注：以上文献为示例，实际引用时需核实真实来源及作者信息。*

背景信息

ALT2. also known as Aldo-Keto Reductase Family 1 Member B1 (AKR1B1), is a key enzyme in the polyol pathway, a glucose metabolism route implicated in diabetic complications. This 36 kDa cytoplasmic protein catalyzes the NADPH-dependent reduction of glucose to sorbitol, which accumulates intracellularly under hyperglycemic conditions. Excessive sorbitol production disrupts osmotic balance and redox homeostasis, contributing to tissue damage in diabetes-related pathologies such as neuropathy, retinopathy, and nephropathy.

The recombinant ALT2 protein, produced through genetic engineering in expression systems like *E. coli* or mammalian cells, enables detailed biochemical characterization and drug discovery. Its crystal structure reveals a (β/α)8 triosephosphate isomerase (TIM) barrel fold with a conserved catalytic tetrad (Tyr48. His110. Trp111. Lys77). Recombinant forms facilitate studies on enzyme kinetics, inhibitor screening, and structure-activity relationships, particularly for diabetes therapeutic development.

Recent research highlights ALT2's broader roles beyond glucose metabolism, including involvement in inflammatory pathways and cellular stress responses. Paradoxically, some studies suggest potential protective functions in cancer progression through detoxification mechanisms. The recombinant protein serves as a critical tool for resolving these dual pathological/beneficial roles and developing tissue-specific modulators.

Current applications include using recombinant ALT2 in high-throughput assays to identify next-generation inhibitors like ranirestat, which show improved selectivity over older drugs such as epalrestat. Ongoing work focuses on understanding isoform-specific functions and designing targeted therapies to mitigate diabetic complications while preserving physiological functions.