| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IRF6 |
| Uniprot No | O14896 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-467aa |
| 氨基酸序列 | MALHPRRVRLKPWLVAQVDSGLYPGLIWLHRDSKRFQIPWKHATRHSPQQ EEENAIFKAWAVETGKYQEGVDDPDPAKWKAQLRCALNKSREFNLMYDGT KEVPMNPVKIYQVCDIPQPQGSIINPGSTGSAPWDEKDNDVDEEDEEDEL DQSQHHVPIQDTFPFLNINGSPMAPASVGNCSVGNCSPEAVWPKTEPLEM EVPQAPIQPFYSSPELWISSLPMTDLDIKFQYRGKEYGQTMTVSNPQGCR LFYGDLGPMPDQEELFGPVSLEQVKFPGPEHITNEKQKLFTSKLLDVMDR GLILEVSGHAIYAIRLCQCKVYWSGPCAPSLVAPNLIERQKKVKLFCLET FLSDLIAHQKGQIEKQPPFEIYLCFGEEWPDGKPLERKLILVQVIPVVAR MIYEMFSGDFTRSFDSGSVRLQISTPDIKDNIVAQLKQLYRILQTQESWQ PMQPTPSMQLPPALPPQ |
| 预测分子量 | 77 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IRF6重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Functional Analysis of IRF6 Mutations in Van der Woude Syndrome"*
**作者**：Kondo S, et al.
**摘要**：该研究通过在大肠杆菌中表达重组IRF6蛋白，结合凝胶迁移实验(EMSA)分析了多个致病突变对IRF6 DNA结合能力的影响，揭示了突变导致腭裂的分子机制。
2. **文献名称**：*"Structural Characterization of Recombinant IRF6 and Its Interaction with SMAD Proteins"*
**作者**：Bailey CM, et al.
**摘要**：利用昆虫细胞系统表达并纯化IRF6重组蛋白，通过X射线晶体学解析其结构，并发现IRF6通过特定结构域与SMAD信号通路蛋白相互作用，调控上皮细胞分化。
3. **文献名称**：*"Production of Recombinant IRF6 for Antibody Development and Epithelial Dysplasia Studies"*
**作者**：Richardson RJ, et al.
**摘要**：研究报道了在哺乳动物细胞(HEK293)中高效表达可溶性IRF6重组蛋白的方法，并利用该蛋白制备特异性抗体，进一步验证了其在口腔上皮发育异常中的功能缺失效应。
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**备注**：以上文献信息为示例性概括，实际研究中请通过PubMed或Web of Science等数据库核对具体文献。IRF6重组蛋白的研究多聚焦于突变功能验证、结构解析及信号通路机制。
Interferon Regulatory Factor 6 (IRF6) is a transcription factor belonging to the IRF family, which plays critical roles in embryonic development, epithelial cell differentiation, and immune regulation. Initially identified for its involvement in orofacial morphogenesis, IRF6 is essential for proper formation of the palate, skin, and other ectodermal tissues. Mutations in the IRF6 gene are strongly associated with human congenital disorders such as Van der Woude syndrome and popliteal pterygium syndrome, characterized by cleft lip/palate and skin abnormalities. Its function extends to regulating keratinocyte proliferation, wound healing, and maintaining epithelial integrity through signaling pathways like TGF-β and p63.
Recombinant IRF6 protein is produced using biotechnology platforms, typically through expression in bacterial (e.g., E. coli) or mammalian cell systems. This engineered protein retains the functional domains of native IRF6. including the conserved DNA-binding domain at the N-terminus and the IRF association domain at the C-terminus. Purification tags (e.g., His-tag) are often added to facilitate isolation. Researchers employ recombinant IRF6 to study its DNA-binding properties, protein-protein interactions, and regulatory mechanisms in developmental biology and disease models.
In biomedical research, recombinant IRF6 serves as a tool to investigate molecular pathways in craniofacial development disorders and epithelial cancers. It enables functional assays, antibody production, and drug screening for potential therapies targeting IRF6-related pathologies. Recent studies also explore its role in modulating immune responses and epithelial-mesenchymal transition, expanding its relevance to cancer metastasis and chronic inflammatory conditions. Quality control involves verifying molecular weight (~55 kDa), phosphorylation status, and DNA-binding activity through techniques like Western blot and electrophoretic mobility shift assays.
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