| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SCFR |
| Uniprot No | P10721 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-516aa |
| 氨基酸序列 | QPSVSPGEPSPPSIHPGKSDLIVRVGDEIRLLCTDPGFVKWTFEILDETNENKQNEWITEKAEATNTGKYTCTNKHGLSNSIYVFVRDPAKLFLVDRSLYGKEDNDTLVRCPLTDPEVTNYSLKGCQGKPLPKDLRFIPDPKAGIMIKSVKRAYHRLCLHCSVDQEGKSVLSEKFILKVRPAFKAVPVVSVSKASYLLREGEEFTVTCTIKDVSSSVYSTWKRENSQTKLQEKYNSWHHGDFNYERQATLTISSARVNDSGVFMCYANNTFGSANVTTTLEVVDKGFINIFPMINTTVFVNDGENVDLIVEYEAFPKPEHQQWIYMNRTFTDKWEDYPKSENESNIRYVSELHLTRLKGTEGGTYTFLVSNSDVNAAIAFNVYVNTKPEILTYDRLVNGMLQCVAAGFPEPTIDWYFCPGTEQRCSASVLPVDVQTLNSSGPPFGKLVVQSSIDSSAFKHNGTVECKAYNDVGKTSAYFNFAFKEQIHPHT |
| 预测分子量 | 82.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SCFR(干细胞因子受体，c-Kit)重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Structural basis for activation of the receptor tyrosine kinase KIT by stem cell factor"*
**作者**：Yuzawa, S., et al.
**摘要**：该研究通过X射线晶体学解析了重组c-Kit受体胞外域与干细胞因子(SCF)的复合物结构，揭示了SCF诱导受体二聚化及激活的分子机制，为靶向药物设计提供了结构依据。
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2. **文献名称**：*"Production of soluble recombinant human stem cell factor receptor (c-Kit) extracellular domain in Pichia pastoris"*
**作者**：Wang, Y., et al.
**摘要**：报道了在毕赤酵母系统中高效表达功能性重组人c-Kit胞外域的方法，并验证其与SCF的特异性结合能力，为开发基于受体阻断的疗法奠定基础。
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3. **文献名称**：*"Targeting oncogenic c-Kit with recombinant immunotoxins in gastrointestinal stromal tumors"*
**作者**：Du, X., et al.
**摘要**：研究构建了靶向突变型c-Kit的重组免疫毒素，通过体外和动物实验证明其能选择性杀伤胃肠道间质瘤细胞，为耐药性肿瘤治疗提供了新策略。
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4. **文献名称**：*"Kinetic and structural analysis of a pathogenic mutant of c-Kit kinase using recombinant protein expression"*
**作者**：Mol, C.D., et al.
**摘要**：通过表达纯化重组c-Kit激酶结构域，分析了致癌突变对激酶活性和药物敏感性的影响，揭示了伊马替尼耐药的部分分子机制。
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这些文献涵盖了重组SCFR蛋白的结构解析、表达优化及治疗应用，反映了其在基础研究与临床开发中的重要性。
**Background of SCFR Recombinant Protein**
Stem Cell Factor Receptor (SCFR), also known as c-Kit or CD117. is a transmembrane tyrosine kinase receptor critical for regulating cell proliferation, survival, and differentiation. It binds to its ligand, Stem Cell Factor (SCF), triggering dimerization and autophosphorylation of the receptor, which activates downstream signaling pathways like PI3K/AKT, MAPK/ERK, and JAK/STAT. These pathways mediate essential biological processes, including hematopoiesis, gametogenesis, melanogenesis, and mast cell development.
SCFR recombinant protein is engineered through genetic modification, typically expressed in mammalian or insect cell systems to ensure proper post-translational modifications. The recombinant form retains the functional domains of native SFR, including the extracellular ligand-binding region, transmembrane segment, and intracellular kinase domain. Its production enables standardized studies on SCF-SCFR interactions, signaling mechanisms, and therapeutic targeting.
Dysregulation of SCFR, such as gain-of-function mutations, is implicated in cancers (e.g., gastrointestinal stromal tumors, acute myeloid leukemia) and other disorders. Recombinant SCFR serves as a tool to study disease mechanisms, screen inhibitors (e.g., imatinib), and develop targeted therapies. Additionally, it supports ex vivo stem cell expansion for regenerative medicine by mimicking physiological SCF signaling.
In research, SCFR recombinant protein is widely used in cell culture, biochemical assays, and structural studies. Its availability accelerates drug discovery and advances understanding of tyrosine kinase receptor biology, offering translational potential in oncology and beyond.
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