| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATP2A2 |
| Uniprot No | P16615 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 314-756aa |
| 氨基酸序列 | VITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCRMFILDRVEGDTCSLNEFTITGSTYAPIGEVHKDDKPVNCHQYDGLVELATICALCNDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTELKGLSKIERANACNSVIKQLMKKEFTLEFSRDRKSMSVYCTPNKPSRTSMSKMFVKGAPEGVIDRCTHIRVGSTKVPMTSGVKQKIMSVIREWGSGSDTLRCLALATHDNPLRREEMHLEDSANFIKYETNLTFVGCVGMLDPPRIEVASSVKLCRQAGIRVIMITGDNKGTAVAICRRIGIFGQDEDVTSKAFTGREFDELNPSAQRDACLNARCFARVEPSHKSKIVEFLQSFDEITAMTGDGVNDAPALKKAEIGIAMGSGTAVAKTASEMVLADDNFSTIVAAVEEGRAIYNNM |
| 预测分子量 | 55.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Crystal Structure of the Calcium Pump of Sarcoplasmic Reticulum at 2.6 Å Resolution"**
作者：Toyoshima, C., et al.
摘要：该研究通过X射线晶体学解析了SERCA2a(ATP2A2编码蛋白)的三维结构，揭示了钙离子结合位点和泵循环机制，为理解其功能及突变相关疾病提供结构基础。
2. **"Expression of Mutant ATP2A2 Proteins in Cultured Cells: Implications for Darier Disease Pathogenesis"**
作者：Dode, L., et al.
摘要：文章利用重组ATP2A2蛋白在HEK293细胞中表达多种致病突变体，发现突变导致钙转运功能缺陷，阐明了Darier病的分子机制。
3. **"Functional Characterization of Recombinant SERCA2b Splice Variant and Its Role in Apoptosis"**
作者：Linde, C., et al.
摘要：研究在大肠杆菌中重组表达了SERCA2b剪接变体，证实其钙转运活性差异，并发现该变体过表达可抑制内质网应激诱导的细胞凋亡。
4. **"High-throughput Screening Assay for SERCA2 Activity Using Recombinant Protein"**
作者：Sugimoto, Y., et al.
摘要：开发了基于重组ATP2A2蛋白的高通量药物筛选平台，鉴定出多个小分子化合物可调节SERCA2活性，为心力衰竭治疗提供潜在先导药物。
(注：以上文献信息为示例性质，实际引用时请核对真实出版物。)
**Background of ATP2A2 Recombinant Protein**
ATP2A2. also known as SERCA2 (sarco/endoplasmic reticulum Ca²⁺-ATPase 2), is a calcium-transporting ATPase encoded by the ATP2A2 gene. It plays a critical role in maintaining calcium homeostasis by actively transporting cytoplasmic Ca²⁺ into the sarco/endoplasmic reticulum (SR/ER), a process essential for cellular functions such as muscle contraction, signal transduction, and protein folding. SERCA2 exists in multiple isoforms (SERCA2a, SERCA2b, SERCA2c) generated via alternative splicing, with SERCA2a predominantly expressed in cardiac and skeletal muscle, while SERCA2b is ubiquitous.
Recombinant ATP2A2 protein is produced using heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to enable functional and structural studies. Its production typically involves cloning the ATP2A2 cDNA into expression vectors, followed by purification via affinity tags. This recombinant tool allows researchers to investigate SERCA2’s enzymatic activity, calcium transport mechanisms, and interactions with regulatory proteins like phospholamban or sarcolipin.
Mutations in ATP2A2 are linked to Darier disease and familial benign chronic pemphigus (Hailey-Hailey disease), autosomal dominant disorders characterized by skin abnormalities due to disrupted Ca²⁺ signaling and ER stress. Recombinant ATP2A2 proteins facilitate the study of these mutations, aiding in understanding their pathogenic effects on calcium handling and cellular integrity.
Additionally, ATP2A2 is a therapeutic target for heart failure and neurodegenerative diseases, as impaired calcium cycling contributes to contractile dysfunction and apoptosis. Recombinant SERCA2 variants are used in drug discovery to identify compounds that enhance its activity or stability, offering potential treatments. Overall, ATP2A2 recombinant protein serves as a vital resource for dissecting calcium-dependent cellular processes and developing targeted therapies.
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