| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDH23 |
| Uniprot No | Q9H251 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDH23重组蛋白的参考文献示例(内容为模拟，仅供参考)：
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1. **文献名称**：*Recombinant CDH23 extracellular domain expression and calcium-dependent adhesion properties*
**作者**：Smith J, et al.
**摘要**：研究报道了CDH23胞外结构域的重组表达与纯化，证实其钙离子依赖性同源二聚化特性，为耳蜗毛细胞黏附机制提供结构依据。
2. **文献名称**：*In vitro reconstitution of CDH23-PCDH15 complex for mechanoelectrical transduction*
**作者**：Kazmierczak P, et al.
**摘要**：利用重组CDH23与PCDH15蛋白，在体外重建了静纤毛尖端连接复合体，揭示二者相互作用对听觉机械电转导的关键作用。
3. **文献名称**：*CDH23 mutations disrupt harmonicin binding in Usher syndrome type 1D*
**作者**：Bork JM, et al.
**摘要**：通过重组蛋白互作实验，发现Usher综合征相关CDH23突变体丧失与harmonicin的结合能力，导致内耳信号复合体组装异常。
4. **文献名称**：*High-yield purification of CDH23 for antibody production and epitope mapping*
**作者**：Xu L, et al.
**摘要**：优化昆虫细胞系统中CDH23重组蛋白表达，获得高纯度产物用于单克隆抗体制备，并鉴定其免疫特异性表位。
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**注**：以上文献为示例性质，实际引用时请核实真实论文信息及数据库来源(如PubMed、ScienceDirect)。建议使用关键词“CDH23 recombinant protein expression/purification/interaction”在学术引擎中检索最新研究。
**Background of CDH23 Recombinant Protein**
CDH23 (Cadherin-Related 23), a member of the cadherin superfamily, is a calcium-dependent transmembrane protein critical for cell-cell adhesion and mechanosensory functions. It is predominantly expressed in sensory hair cells of the inner ear and photoreceptors in the retina. Structurally, CDH23 contains 27 extracellular cadherin repeats, a transmembrane domain, and a cytoplasmic tail. Its extracellular domain mediates homophilic and heterophilic interactions, while the cytoplasmic region binds to scaffolding proteins like harmonin, forming molecular complexes essential for sensory signal transduction.
Mutations in the *CDH23* gene are linked to Usher syndrome type 1D (USH1D), a genetic disorder characterized by congenital deafness, vestibular dysfunction, and progressive vision loss. CDH23 also plays a pivotal role in the assembly of stereocilia tip links—filamentous structures that gate mechanotransduction channels in hair cells. These tip links, composed of CDH23 and protocadherin-15 (PCDH15), convert mechanical sound waves into electrical signals, enabling hearing.
Recombinant CDH23 protein is engineered using expression systems (e.g., mammalian or insect cells) to produce functional extracellular or full-length domains for research and therapeutic applications. It serves as a tool to study protein-protein interactions, structural dynamics, and pathogenic mechanisms underlying hearing loss. Additionally, it aids in drug screening for Usher syndrome and age-related hearing disorders. Purified CDH23 recombinant protein is often tagged (e.g., His-tag) for ease of isolation and characterized via SDS-PAGE, Western blot, or binding assays to confirm activity.
Overall, CDH23 recombinant protein bridges basic research and clinical innovation, offering insights into sensory biology and potential therapies for auditory and visual impairments.
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