| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF440 |
| Uniprot No | Q8IYI8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-595 aa |
| 活性数据 | MDPVAFKDVA VNFTQEEWAL LDISQRKLYR EVMLETFRNL TSLGKRWKDQ NIEYEHQNPR RNFRSLIEEK VNEIKDDSHC GETFTPVPDD RLNFQEKKAS PEVKSCESFV CGEVGLGNSS FNMNIRGDIG HKAYEYQEYG PKPCKCQQPK KAFRYRPSFR TQERDHTGEK PNACKVCGKT FISHSSVRRH MVMHSGDGPY KCKFCGKAFH CLRLYLIHER IHTGEKPCEC KQCGKSFSYS ATHRIHKRTH TGEKPYEYQE CGKAFHSPRS YRRHERIHMG EKAYQCKECG KAFTCPRYVR IHERTHSRKN LYECKQCGKA LSSLTSFQTH VRLHSGERPY ECKICGKDFC SVNSFQRHEK IHSGEKPYKC KQCGKAFPHS SSLRYHERTH TGEKPYECKQ CGKAFRSASH LRVHGRTHTG EKPYECKECG KAFRYVNNLQ SHERTQTHIR IHSGERRYKC KICGKGFYCP KSFQRHEKTH TGEKLYECKQ RSVVPSVVPV PFDIMKGLTL ERSPINASNV GKPSELCQSF ECMVGLTLKR NPMSVSNDGK PSDLPHTFEY VVGHTMERSP MHVRNVGNPS DLPRTFEFMK GHKHT |
| 分子量 | 69.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人ZNF440蛋白的研究目前公开的文献较为有限，以下为基于锌指蛋白家族研究和重组蛋白技术撰写的假设性参考文献示例(非真实文献，仅供格式参考)：
1. **"Expression and Functional Analysis of Recombinant Human ZNF440 in HEK293 Cells"**
*作者：Li X, et al. (2022)*
摘要：本研究成功在HEK293细胞中表达了带His标签的重组人ZNF440蛋白，验证其核定位特性，并通过ChIP-seq发现其可能结合到与细胞周期调控相关的基因启动子区域。
2. **"Structural Characterization of ZNF440 Zinc Finger Domain by NMR Spectroscopy"**
*作者：Zhang Y, et al. (2021)*
摘要：首次报道了ZNF440蛋白锌指结构域的核磁共振结构，揭示了其与DNA相互作用的潜在位点，为研究其转录调控功能提供结构基础。
3. **"ZNF440 Knockdown Suppresses Migration in Colorectal Cancer Cells"**
*作者：Wang T, et al. (2023)*
摘要：通过siRNA敲低ZNF440导致结直肠癌细胞迁移能力下降，重组ZNF440蛋白的添加可部分恢复表型，提示其可能在肿瘤转移中发挥作用。
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**备注**：
ZNF440作为锌指蛋白家族成员，其真实研究可能聚焦于基因调控或疾病关联方向。建议通过以下途径获取最新文献：
1. **PubMed/NCBI**：搜索 `"ZNF440" AND (recombinant OR structure OR function)`
2. **Google Scholar**：使用关键词 `recombinant ZNF440 protein expression`
3. **UniProt数据库**：查看ZNF440的注释信息(ID: [Q9UJU6](https://www.uniprot.org/uniprotkb/Q9UJU6/entry))获取潜在研究线索。
Zinc finger protein 440 (ZNF440) is a member of the zinc finger protein family, characterized by conserved Cys₂His₂ (C2H2)-type zinc finger domains that facilitate DNA or RNA binding. As a transcription factor, ZNF440 is hypothesized to regulate gene expression by interacting with specific DNA sequences, though its precise biological functions remain understudied compared to other zinc finger proteins. The human ZNF440 gene is located on chromosome 19 (19q13.43) and encodes a protein with multiple tandem zinc finger motifs, suggesting potential roles in transcriptional regulation, chromatin remodeling, or cellular differentiation.
Recombinant human ZNF440 protein is typically produced using expression systems like *E. coli* or mammalian cell cultures, followed by purification via affinity chromatography. This engineered protein enables functional studies to decipher its DNA-binding specificity, protein-protein interactions, and regulatory targets. Emerging research indicates zinc finger proteins may be involved in developmental processes and disease pathways, including cancer or neurological disorders, positioning ZNF440 as a candidate for exploring these connections. However, its exact physiological roles, downstream genes, and relevance to human pathologies require further investigation. Recombinant ZNF440 tools are critical for unraveling its molecular mechanisms and evaluating its potential as a therapeutic or diagnostic target.
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