| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF435 |
| Uniprot No | Q9H4T2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-348 aa |
| 活性数据 | MTTALEPEDQ KGLLIIKAED HYWGQDSSSQ KCSPHRRELY RQHFRKLCYQ DAPGPREALT QLWELCRQWL RPECHTKEQI LDLLVLEQFL SILPKDLQAW VRAHHPETGE EAVTVLEDLE RELDEPGKQV PGNSERRDIL MDKLAPLGRP YESLTVQLHP KKTQLEQEAG KPQRNGDKTR TKNEELFQKE DMPKDKEFLG EINDRLNKDT PQHPKSKDII ENEGRSEWQQ RERRRYKCDE CGKSFSHSSD LSKHRRTHTG EKPYKCDECG KAFIQRSHLI GHHRVHTGVK PYKCKECGKD FSGRTGLIQH QRIHTGEKPY ECDECGRPFR VSSALIRHQR IHTANKLY |
| 分子量 | 40.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZNF435蛋白的示例参考文献(均为虚构，仅供格式参考)：
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1. **"Structural analysis of human ZNF435 and its DNA-binding specificity"**
*Zhang, L. et al. (2018). Journal of Molecular Biology.*
摘要：解析了ZNF435蛋白的晶体结构，发现其锌指结构域特异性结合DNA的AT富集区，可能与基因转录调控相关。
2. **"Recombinant ZNF435 inhibits tumor growth by modulating apoptosis pathways"**
*Chen, W. & Park, S. (2020). Oncology Reports.*
摘要：实验表明，重组ZNF435蛋白在癌细胞中过表达可激活促凋亡蛋白BAX，抑制小鼠模型中的肿瘤增殖。
3. **"Functional characterization of ZNF435 as a novel regulator of lipid metabolism"**
*Gupta, R. et al. (2021). Cell Metabolism.*
摘要：通过基因敲除技术证明，ZNF435参与脂肪酸氧化通路，重组蛋白可改善高脂饮食小鼠的代谢紊乱。
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*注：以上文献为模拟内容，实际研究中请通过PubMed/Google Scholar以“ZNF435”或“recombinant ZNF435”为关键词查找真实文献。*
Zinc finger protein 435 (ZNF435) is a member of the Kruppel-associated box (KRAB) domain-containing zinc finger protein (ZNF) family, which constitutes one of the largest groups of transcription regulators in humans. Characterized by multiple C2H2-type zinc finger motifs, ZNF435 is hypothesized to bind specific DNA sequences, modulating gene expression through epigenetic mechanisms or direct transcriptional activation/repression. While its exact biological roles remain under investigation, ZNF435 is believed to participate in cellular processes such as differentiation, proliferation, and chromatin remodeling. Genomic studies suggest potential links to developmental disorders and cancers, though functional evidence is still emerging.
Recombinant human ZNF435 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables *in vitro* studies to decipher its molecular interactions, DNA-binding specificity, and structural features. The protein typically includes epitope tags (e.g., His-tag) for purification and detection. Current research focuses on mapping its target genes, characterizing post-translational modifications, and exploring its regulatory networks in health and disease. As a relatively understudied ZNF member, ZNF435 represents a frontier in understanding the diversity of zinc finger-mediated gene regulation, with implications for developing therapeutic strategies targeting transcription factors.
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