| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZDHHC17 |
| Uniprot No | Q8IUH5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-632 aa |
| 活性数据 | MQREEGFNTKMADGPDEYDTEAGCVPLLHPEEIKPQSHYNHGYGEPLGRKTHIDDYSTWDIVKATQYGIYERCRELVEAGYDVRQPDKENVTLLHWAAINNRIDLVKYYISKGAIVDQLGGDLNSTPLHWATRQGHLSMVVQLMKYGADPSLIDGEGCSCIHLAAQFGHTSIVAYLIAKGQDVDMMDQNGMTPLMWAAYRTHSVDPTRLLLTFNVSVNLGDKYHKNTALHWAVLAGNTTVISLLLEAGANVDAQNIKGESALDLAKQRKNVWMINHLQEARQAKGYDNPSFLRKLKADKEFRQKVMLGTPFLVIWLVGFIADLNIDSWLIKGLMYGGVWATVQFLSKSFFDHSMHSALPLGIYLATKFWMYVTWFFWFWNDLNFLFIHLPFLANSVALFYNFGKSWKSDPGIIKATEEQKKKTIVELAETGSLDLSIFCSTCLIRKPVRSKHCGVCNRCIAKFDHHCPWVGNCVGAGNHRYFMGYLFFLLFMICWMIYGCISYWGLHCETTYTKDGFWTYITQIATCSPWMFWMFLNSVFHFMWVAVLLMCQMYQISCLGITTNERMNARRYKHFKVTTTSIESPFNHGCVRNIIDFFEFRCCGLFRPVIVDWTRQYTIEYDQISGSGYQLV |
| 分子量 | 95.26 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与ZDHHC17蛋白相关的3篇参考文献及其摘要概括：
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1. **文献名称**: *ZDHHC17-mediated palmitoylation regulates huntingtin stability and trafficking*
**作者**: Yano H et al. (2015)
**摘要**: 研究揭示了ZDHHC17通过棕榈酰化修饰Huntingtin蛋白(HTT)，调控其稳定性和亚细胞定位，突变可能破坏HTT功能，进而参与亨廷顿病的病理过程。
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2. **文献名称**: *ZDHHC17 controls synaptic plasticity by palmitoylating the postsynaptic protein PSD-95*
**作者**: Shirai Y et al. (2018)
**摘要**: 发现ZDHHC17特异性催化突触后支架蛋白PSD-95的棕榈酰化修饰，影响突触强度和神经元可塑性，缺失会导致小鼠学习记忆能力受损，提示其在神经发育中的作用。
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3. **文献名称**: *Structural basis of ZDHHC17 substrate recognition in neurodegenerative disease pathways*
**作者**: Liu Y et al. (2020)
**摘要**: 通过冷冻电镜解析了ZDHHC17的酶结构，明确了其与底物(如SNAP25)的结合机制，为开发针对ZDHHC17异常导致的神经退行性疾病药物提供结构基础。
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如需更多文献，可进一步补充特定研究方向(如癌症、代谢疾病等)。
ZDHHC17 (zinc finger DHHC-type containing 17) is a member of the DHHC family of palmitoyltransferases, enzymes that catalyze protein palmitoylation—a post-translational lipid modification critical for membrane anchoring, protein trafficking, and signal transduction. This protein contains a conserved DHHC (Asp-His-His-Cys) catalytic domain and an N-terminal ankyrin-repeat domain, which facilitates substrate recognition. ZDHHC17 is predominantly localized in the Golgi apparatus and plasma membrane, where it regulates the function of diverse substrates, including synaptic proteins (e.g., SNAP-25) and disease-related targets like huntingtin.
Studies implicate ZDHHC17 in neurological processes, particularly synaptic plasticity and neurodevelopment. Dysregulation of its activity has been linked to neurodegenerative disorders, such as Alzheimer’s and Huntington’s diseases, due to impaired palmitoylation of disease-associated proteins. It also plays roles in cancer progression, influencing cell adhesion, metastasis, and apoptosis resistance.
Recombinant human ZDHHC17 protein, produced via heterologous expression systems (e.g., E. coli, mammalian cells), enables mechanistic studies of its enzymatic activity, substrate interactions, and regulatory pathways. Its reconstitution in vitro aids in screening modulators for therapeutic development. However, challenges remain in understanding its substrate specificity and context-dependent regulation. Current research leverages structural biology and CRISPR-based approaches to unravel its pathophysiological roles, highlighting its potential as a therapeutic target for neurological and oncological conditions.
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