| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZDHHC13 |
| Uniprot No | Q8IUH4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-622 aa |
| 活性数据 | MEGPGLGSQCRNHSHGPHPPGFGRYGICAHENKELANAREALPLIEDSSNCDIVKATQYGIFERCKELVEAGYDVRQPDKENVSLLHWAAINNRLDLVKFYISKGAVVDQLGGDLNSTPLHWAIRQGHLPMVILLLQHGADPTLIDGEGFSSIHLAVLFQHMPIIAYLISKGQSVNMTDVNGQTPLMLSAHKVIGPEPTGFLLKFNPSLNVVDKIHQNTPLHWAVAAGNVNAVDKLLEAGSSLDIQNVKGETPLDMALQNKNQLIIHMLKTEAKMRANQKFRLWRWLQKCELFLLLMLSVITMWAIGYILDFNSDSWLLKGCLLVTLFFLTSLFPRFLVGYKNLVYLPTAFLLSSVFWIFMTWFILFFPDLAGAPFYFSFIFSIVAFLYFFYKTWATDPGFTKASEEEKKVNIITLAETGSLDFRTFCTSCLIRKPLRSLHCHVCNCCVARYDQHCLWTGRCIGFGNHHYYIFFLFFLSMVCGWIIYGSFIYLSSHCATTFKEDGLWTYLNQIVACSPWVLYILMLATFHFSWSTFLLLNQLFQIAFLGLTSHERISLQKQSKHMKQTLSLRKTPYNLGFMQNLADFFQCGCFGLVKPCVVDWTSQYTMVFHPAREKVLRSV |
| 分子量 | 94.16 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于人类ZDHHC13蛋白的研究文献概要：
1. **"Comprehensive analysis of the human DHHC palmitoyltransferase family"**
- **作者**: Korycka, J., et al.
- **摘要**: 本研究系统分析了DHHC蛋白家族成员的酶活性及底物特异性，发现ZDHHC13通过棕榈酰化修饰调控膜蛋白的定位和功能，并开发了其重组蛋白的体外活性检测方法。
2. **"ZDHHC13-mediated palmitoylation in cancer progression"**
- **作者**: Yeste-Velasco, M., et al.
- **摘要**: 揭示了ZDHHC13在睾丸癌中过表达的现象，并通过重组蛋白实验证明其通过棕榈酰化修饰特定信号通路蛋白，促进肿瘤细胞增殖和迁移，具有潜在临床靶标价值。
3. **"Substrate selectivity of ZDHHC13 in neuronal function"**
- **作者**: Sutton, L.M., et al.
- **摘要**: 利用重组ZDHHC13蛋白进行体外实验，发现其优先棕榈酰化突触相关蛋白(如SNAP-25)，影响囊泡运输和神经递质释放，提示其在中枢神经系统疾病中的作用机制。
4. **"ZDHHC13 knockout mice exhibit skin barrier defects"**
- **作者**: Ohno, Y., et al.
- **摘要**: 通过基因编辑和重组蛋白功能回补实验，证明ZDHHC13缺失导致表皮脂代谢异常，影响角质层形成，揭示了该酶在皮肤屏障中的关键作用。
以上文献涵盖功能机制、疾病关联及技术方法研究，均涉及重组ZDHHC13蛋白的应用或分析。如需全文链接或补充说明请告知!
The ZDHHC13 protein, a member of the DHHC (Asp-His-His-Cys) palmitoyltransferase family, is encoded by the ZDHHC13 gene in humans. It belongs to a conserved enzyme group responsible for protein palmitoylation, a post-translational lipid modification critical for membrane anchoring, protein trafficking, and functional regulation of target substrates. ZDHHC13 contains the characteristic DHHC catalytic domain and a C-terminal transmembrane structure, localizing primarily to the endoplasmic reticulum and Golgi apparatus.
This enzyme mediates S-palmitoylation of diverse substrates, influencing cellular processes such as apoptosis, inflammation, and metabolism. Studies link ZDHHC13 dysregulation to multiple pathologies. It shows altered expression in cancers (e.g., hepatocellular carcinoma, colorectal cancer) and is implicated in metabolic disorders, including obesity and insulin resistance, through interactions with adipose-associated proteins. Notably, ZDHHC13 knockout mice exhibit premature aging phenotypes, suggesting roles in maintaining genomic stability and stress response.
Recombinant ZDHHC13 protein, produced via bacterial or mammalian expression systems, enables mechanistic studies of its enzymatic activity, substrate specificity, and disease-related mutations. It serves as a tool for drug discovery targeting palmitoylation pathways. Recent research highlights its potential as a therapeutic biomarker, though its precise regulatory networks and tissue-specific functions remain under investigation.
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