| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZCWPW1 |
| Uniprot No | Q9H0M4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-477 aa |
| 活性数据 | MGSGLDFAETSCAQPVVSTQSDKEPGITASAADTDNANGEEVPHTQEISVSWEGEAAPEIRTSKLGQPDPAPSKKKSNRLTLSKRKKEAQDEKVEKTQGGHEHRQEDRLKKTVQDHSQIRDQQKGEISGFGQCLVWVQCSFPNCGKWRRLCGNIDPSVLPDNWSCDQNTDVQYNRCDIPEETWTGLESDVAYASYIPGSIIWAKQYGYPWWPGMIESDPDLGEYFLFTSHLDSLPSKYHVTFFGETVSRAWIPVNMLKNFQELSLELSVMKKRRNDCSQKLGVALMMAQEAEQISIQERVNLFGFWSRFNGSNSNGERKDLQLSGLNSPGSCLEKKEKEEELEKEEGEKTDPILPIRKRVKIQTQKTKPRGPKKKFKAPQSKALAASFSEGKEVRTVPKNLGLSACKGACPSSAKEEPRHREPLTQEAGSVPLEDEASSDLDLEQLMEDVGRELGQSGELQHSNSDGEDFPVALFGK |
| 分子量 | 79.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZCWPW1蛋白的参考文献示例(内容为模拟虚构，供参考)：
1. **《ZCWPW1 is essential for epigenetic reprogramming in mammalian meiosis》**
- 作者：Chen L, et al.
- 摘要：阐明ZCWPW1在哺乳动物减数分裂中通过识别组蛋白H3K4me3修饰，促进同源染色体配对和DNA双链断裂修复的作用机制。
2. **《Structural basis of ZCWPW1 as a reader of histone modification in CRISPR-Cas9 editing》**
- 作者：Zhang Y, et al.
- 摘要：解析ZCWPW1蛋白结构，揭示其作为表观遗传"阅读器"结合H3K4me3的结构基础，并探讨其在CRISPR基因编辑中提高靶向效率的应用。
3. **《ZCWPW1 deficiency causes impaired spermatogenesis and male infertility in mice》**
- 作者：Wang H, et al.
- 摘要：通过敲除小鼠模型证明ZCWPW1缺失导致精子形成障碍，证实其在生殖细胞中调控减数分裂特异性基因表达的功能。
4. **《A dual role of ZCWPW1 in DNA repair and epigenetic silencing》**
- 作者：Kumar R, et al.
- 摘要：揭示ZCWPW1通过协调DNA损伤修复和启动子区甲基化修饰，参与维持基因组稳定性及肿瘤抑制机制。
(注：以上文献为示例性内容，实际引用请查阅PubMed等数据库获取真实论文。)
ZCWPW1 (Zinc Finger CW-Type and PWWP Domain-Containing Protein 1) is a human protein encoded by the *ZCWPW1* gene, notable for its dual structural domains. The N-terminal region contains a CW-type zinc finger motif, which facilitates chromatin interaction and epigenetic regulation, while the C-terminal harbors a PWWP domain, typically involved in binding methylated histones or DNA. This domain architecture suggests roles in chromatin remodeling, DNA repair, or transcriptional regulation. Studies link ZCWPW1 to meiosis, particularly in facilitating the pairing of homologous chromosomes by recognizing epigenetic marks like H3K4me3 during gametogenesis. Its interaction with recombination hotspots highlights its importance in genetic diversity and genomic stability.
Recombinant ZCWPW1 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. It enables in vitro analysis of its DNA-binding specificity, enzymatic activity, and interactions with chromatin modifiers. Researchers employ it to explore its role in CRISPR-based epigenome editing, gene regulation, or as a tool to decipher molecular mechanisms underlying infertility or developmental disorders. Additionally, recombinant ZCWPW1 serves as an antigen for antibody development, aiding in disease biomarker discovery. Further investigations aim to clarify its therapeutic potential in reproductive health and diseases linked to chromatin dysregulation.
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