| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZCCHC4 |
| Uniprot No | Q9H5U6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-205 aa |
| 活性数据 | MEVVLPLDPAVPAPLCPHGPTLLFVKVTQGKEETRRFYACSACRDRKDCNFFQWEDEKLSGARLAAREAHNRRCQPPLSRTQCVERYLKFIELPLTQRKFCQTCQQLLLPDDWGQHSEHQVLGNVSITQLRRPSQLLYPLENKKTNAQYLFADRSCQFLVDLLSALGFRRVLCVGTPRLHELIKLTASGDKKSNIKSLLLDIDFR |
| 分子量 | 50 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZCCHC4蛋白的3篇参考文献及其摘要内容概要：
1. **文献名称**: **"ZCCHC4 mediates rRNA methylation to promote hepatocellular carcinoma progression"**
**作者**: Zhang Y, et al.
**摘要**: 该研究发现ZCCHC4作为28S rRNA的m6A甲基转移酶，在肝癌中高表达，通过促进核糖体生物合成和蛋白质翻译驱动肿瘤生长，揭示了其在肝癌中的致癌机制。
2. **文献名称**: **"Structural basis of RNA methylation by ZCCHC4 in human ribosome biogenesis"**
**作者**: Wang L, et al.
**摘要**: 通过冷冻电镜解析了ZCCHC4蛋白的结构，阐明其催化rRNA特定位点甲基化的分子机制，并利用重组ZCCHC4验证了其酶活功能，为靶向干预提供了结构基础。
3. **文献名称**: **"ZCCHC4 modulates the m6A methylation landscape and regulates embryonic stem cell self-renewal"**
**作者**: Li X, et al.
**摘要**: 研究证明ZCCHC4通过调控关键多能性转录因子mRNA的m6A修饰，维持胚胎干细胞的自我更新，重组ZCCHC4蛋白的体外实验证实了其对RNA甲基化的直接作用。
注：以上文献为示例，实际引用时需核实具体来源及真实性。建议通过PubMed或Web of Science以关键词“ZCCHC4”+“methyltransferase”/“cancer”/“structure”检索最新研究。
Recombinant ZCCHC4 protein is a human zinc finger CCHC-type containing protein 4. primarily studied for its role in RNA metabolism and post-transcriptional regulation. As a member of the ZCCHC family, it contains characteristic CCHC zinc finger motifs that facilitate RNA-binding and protein-protein interactions. ZCCHC4 has been identified as a putative methyltransferase, implicated in the N6-methyladenosine (m6A) modification of RNA—a dynamic epigenetic marker influencing RNA stability, splicing, and translation.
Recent studies highlight its involvement in ribosome biogenesis and DNA damage response pathways. For instance, ZCCHC4 selectively methylates 28S ribosomal RNA at a specific site (A4220), critical for ribosome assembly and translational fidelity. Dysregulation of ZCCHC4 is linked to pathological conditions, including hepatocellular carcinoma, where its overexpression correlates with poor prognosis and tumor proliferation. Additionally, it interacts with viral RNA during infections, suggesting roles in host-pathogen interactions.
Recombinant ZCCHC4 is commonly expressed in E. coli or mammalian systems for functional studies, retaining enzymatic activity and structural integrity. Its applications span in vitro methylation assays, RNA-protein interaction studies, and drug discovery targeting RNA-modifying enzymes. Emerging research also explores its potential as a biomarker or therapeutic target in cancers and viral diseases. Ongoing investigations aim to clarify its substrate specificity, regulatory networks, and broader roles in cellular stress responses.
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