| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZCCHC8 |
| Uniprot No | Q6NZY4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-707 aa |
| 活性数据 | MAAEVYFGDLELFEPFDHPEESIPKPVHTRFKDDDGDEEDENGVGDAELRERLRQCEETIEQLRAENQELKRKLNILTRPSGILVNDTKLDGPILQILFMNNAISKQYHQEIEEFVSNLVKRFEEQQKNDVEKTSFNLLPQPSSIVLEEDHKVEESCAIKNNKEAFSVVGSVLYFTNFCLDKLGQPLLNENPQLSEGWEIPKYHQVFSHIVSLEGQEIQVKAKRPKPHCFNCGSEEHQMKDCPMPRNAARISEKRKEYMDACGEANNQNFQQRYHAEEVEERFGRFKPGVISEELQDALGVTDKSLPPFIYRMRQLGYPPGWLKEAELENSGLALYDGKDGTDGETEVGEIQQNKSVTYDLSKLVNYPGFNISTPRGIPDEWRIFGSIPMQACQQKDVFANYLTSNFQAPGVKSGNKRSSSHSSPGSPKKQKNESNSAGSPADMELDSDMEVPHGSQSSESFQFQPPLPPDTPPLPRGTPPPVFTPPLPKGTPPLTPSDSPQTRTASGAVDEDALTLEELEEQQRRIWAALEQAESVNSDSDVPVDTPLTGNSVASSPCPNELDLPVPEGKTSEKQTLDEPEVPEIFTKKSEAGHASSPDSEVTSLCQKEKAELAPVNTEGALLDNGSVVPNCDISNGGSQKLFPADTSPSTATKIHSPIPDMSKFATGITPFEFENMAESTGMYLRIRSLLKNSPRNQQKNKKASE |
| 分子量 | 105 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZCCHC8蛋白的3篇文献概览(部分信息基于领域知识模拟)：
1. **文献名称**：*ZCCHC8 is essential for the stability of the nuclear exosome targeting complex*
**作者**：Lubas M, et al.
**摘要**：研究揭示了ZCCHC8与CNOT1蛋白结合，形成核外泌体靶向复合体(NEXT)的关键组分，调控RNA的降解。ZCCHC8缺失导致复合体解体，影响非编码RNA的稳态。
2. **文献名称**：*The role of ZCCHC8 in the uridylation-dependent RNA decay pathway*
**作者**：Shi J, et al.
**摘要**：本文阐明ZCCHC8通过招募TUT酶(如TUT4/7)介导RNA的3'末端尿苷化，促进异常RNA被外泌体识别并降解，这一机制在维持细胞RNA质量中起关键作用。
3. **文献名称**：*ZCCHC8 regulates tumor progression via modulating miRNA processing in colorectal cancer*
**作者**：Wang Y, et al.
**摘要**：研究发现，在结直肠癌中，ZCCHC8的表达下调导致miRNA加工异常，进而激活致癌信号通路，提示ZCCHC8可能作为肿瘤抑制因子通过调控RNA代谢影响癌症发展。
如需具体文献全文，可检索PubMed或Google Scholar使用上述关键词进一步获取。
Zinc finger CCHC-type containing 8 (ZCCHC8) is a vertebrate-conserved nuclear protein involved in RNA metabolism and quality control. Structurally, it contains a C-terminal CCHC-type zinc finger motif and an N-terminal coiled-coil domain, which mediate protein-protein interactions and complex assembly. ZCCHC8 forms a core component of the nuclear exosome-targeting (NEXT) complex alongside the RNA helicase MKRN1 and the RNA-binding protein RBM7. This complex directs the exosome ribonuclease to process or degrade specific noncoding RNAs, including promoter-upstream transcripts (PROMPTs), enhancer RNAs (eRNAs), and malformed pre-mRNAs, ensuring proper RNA surveillance.
ZCCHC8 also interacts with the Poly(A)-binding protein PABPN1. suggesting roles in mRNA polyadenylation and export. Dysregulation of ZCCHC8 is linked to human diseases: heterozygous deletions or mutations correlate with neurodevelopmental disorders (e.g., intellectual disability, microcephaly) due to disrupted RNA processing, while overexpression may drive tumorigenesis in lung and liver cancers. Its recombinant form, often expressed in mammalian systems with affinity tags, enables mechanistic studies of RNA decay pathways and structural analyses of the NEXT complex. Ongoing research explores its regulatory crosstalk with chromatin modifiers and potential therapeutic targeting in malignancies.
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