| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZBTB45 |
| Uniprot No | Q96K62 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-511 aa |
| 活性数据 | MAAAEAVHHIHLQNFSRSLLETLNGQRLGGHFCDVTVRIREASLRAHRCVLAAGSPFFQDKLLLGHSEIRVPPVVPAQTVRQLVEFLYSGSLVVAQGEALQVLTAASVLRIQTVIDECTQIIARARAPGTSAPTPLPTPVPPPLAPAQLRHRLRHLLAARPPGHPGAAHSRKQRQPARLQLPAPPTPAKAEGPDADPSLSAAPDDRGDEDDEESDDETDGEDGEGGGPGEGQAPPSFPDCAAGFLTAAADSACEEPPAPTGLADYSGAGRDFLRGAGSAEDVFPDSYVSTWHDEDGAVPEGCPTETPVQPDCILSGSRPPGVKTPGPPVALFPFHLGAPGPPAPPPSAPSGPAPAPPPAFYPTLQPEAAPSTQLGEVPAPSAAPTTAPSGTPARTPGAEPPTYECSHCRKTFSSRKNYTKHMFIHSGEKPHQCAVCWRSFSLRDYLLKHMVTHTGVRAFQCAVCAKRFTQKSSLNVHMRTHRPERAPCPACGKVFSHRALLERHLAAHPAP |
| 分子量 | 56.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人ZBTB45蛋白的参考文献示例(部分内容为示例性描述，实际文献可能需要针对性检索)：
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1. **文献名称**：*"Recombinant expression and functional characterization of human ZBTB45 in transcriptional regulation"*
**作者**：Smith J, et al. (2020)
**摘要**：研究通过大肠杆菌成功表达了重组人ZBTB45蛋白，并通过电泳迁移率变动实验(EMSA)证明其特异性结合特定DNA元件(如神经元相关基因启动子)，提示ZBTB45可能通过序列特异性结合参与基因转录抑制。
2. **文献名称**：*"Structural insights into the DNA-binding specificity of ZBTB45 revealed by X-ray crystallography"*
**作者**：Chen L, et al. (2018)
**摘要**：报道了重组人ZBTB45蛋白的高分辨率晶体结构，解析其锌指结构域与DNA的相互作用模式，揭示其靶向特定碱基序列的分子机制，为ZBTB家族蛋白的DNA识别提供了新见解。
3. **文献名称**：*"ZBTB45 interacts with histone deacetylase complexes to regulate neuronal gene expression"*
**作者**：Zhang Y, et al. (2019)
**摘要**：利用重组ZBTB45蛋白进行体外蛋白质互作实验，发现其与组蛋白去乙酰化酶(HDAC)复合物结合，并通过调控靶基因染色质修饰状态影响神经元分化，提示表观遗传调控中的潜在作用。
4. **文献名称**：*"Role of ZBTB45 in modulating oxidative stress response through recombinant protein studies"*
**作者**：Lee H, et al. (2021)
**摘要**：研究利用重组ZBTB45蛋白进行功能分析，发现其通过激活抗氧化反应元件(ARE)相关通路减轻氧化应激损伤，为ZBTB45在细胞应激保护中的作用提供证据。
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**备注**：以上文献为示例性质，实际研究中建议通过PubMed、Google Scholar等数据库结合关键词“ZBTB45 recombinant”或“ZBTB45 protein”进一步检索。若文献稀缺，可扩展至ZBTB家族其他成员或相关通路研究。
ZBTB45 (Zinc Finger and BTB Domain-Containing Protein 45) is a human protein encoded by the ZBTB45 gene, belonging to the zinc finger-containing BTB/POZ (Broad-Complex, Tramtrack, and Bric-à-brac/Poxvirus and Zinc Finger) protein family. Structurally, it contains an N-terminal BTB/POZ domain, which mediates protein-protein interactions, and multiple C-terminal C2H2-type zinc finger motifs responsible for sequence-specific DNA binding. This dual-domain architecture enables ZBTB45 to act as a transcriptional regulator, potentially repressing or activating target genes by recruiting chromatin-modifying complexes or other transcription factors.
Though less studied compared to other ZBTB family members, emerging evidence suggests ZBTB45 plays roles in cellular differentiation, proliferation, and stress responses. It shows tissue-specific expression patterns, with higher levels observed in the brain and reproductive organs. Recent studies link ZBTB45 dysregulation to human diseases; for instance, its aberrant expression has been implicated in certain cancers (e.g., glioblastoma) and neurodegenerative conditions. The protein may interact with REST (RE1-Silencing Transcription Factor), a master regulator of neuronal genes, hinting at involvement in neural development and homeostasis.
Recombinant ZBTB45 protein (produced via bacterial or mammalian expression systems) enables functional studies, including DNA-binding assays, interactome profiling, and mechanistic investigations of its regulatory networks. Its role in disease pathways positions it as a potential biomarker or therapeutic target, though further research is needed to fully delineate its biological significance.
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