| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZAK |
| Uniprot No | Q9NYL2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-455 aa |
| 活性数据 | MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYGVILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVVIAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLEGLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTSLPDKCNSFLHNKAEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLLPLAARMSEESYFESKTEESNSAEMSCQITATSNGEGHGMNPSLQAMMLMGFGDIFSMNKAGAVMHSGMQINMQAKQNSSKTTSKRRGKKVNMALGFSDFDLSEGDDDDDDDGEEEDNDMDNSE |
| 分子量 | 75.79 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人ZAK蛋白相关的研究文献示例(文献信息为虚构，仅用于示例)：
1. **文献名称**：*The role of recombinant human ZAKα in stress-activated kinase pathways*
**作者**：Smith J, et al.
**摘要**：本研究通过昆虫细胞表达系统成功重组表达并纯化出具有活性的ZAKα蛋白，证实其在激活JNK/p38通路中响应核糖体应激的分子机制，为ZAK介导的细胞凋亡研究提供工具。
2. **文献名称**：*Structural characterization of recombinant ZAK kinase domain using X-ray crystallography*
**作者**：Chen L, et al.
**摘要**：利用大肠杆菌系统重组表达人源ZAK激酶结构域，通过晶体学解析其三维结构，揭示了ZAK底物结合位点的关键氨基酸，为靶向抑制剂设计奠定基础。
3. **文献名称**：*Recombinant ZAK protein as a biosensor for DNA damage signaling*
**作者**：Wang Y, et al.
**摘要**：开发了一种基于重组ZAK蛋白的体外检测体系，证明ZAK可特异性识别紫外线引起的DNA损伤信号，并通过MAPK级联反应触发下游修复基因表达。
4. **文献名称**：*Functional analysis of ZAK isoforms in apoptosis using recombinant proteins*
**作者**：Kumar R, et al.
**摘要**：通过哺乳动物细胞重组表达ZAK两种剪接变体(ZAKα和ZAKβ)，发现二者在TGF-β信号通路中具有相反的促凋亡和抗凋亡功能差异。
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**备注**：以上文献为示例性内容，实际研究中需通过PubMed或Web of Science等平台检索具体文献(关键词：recombinant ZAK protein, human ZAK expression, ZAK kinase signaling)。
Recombinant human ZAK protein, also known as leucine zipper- and sterile alpha motif-containing kinase (ZAKα), is a key signaling molecule belonging to the mitogen-activated protein kinase kinase kinase (MAP3K) family. It plays a central role in cellular stress response pathways, particularly in mediating reactions to ribotoxic stress, DNA damage, and cytoskeletal disruption. ZAK contains an N-terminal kinase domain and a C-terminal leucine zipper motif, enabling dimerization and interaction with downstream targets. Upon activation, it phosphorylates components of the SAPK/JNK and p38 MAPK pathways, triggering apoptosis or cell cycle arrest.
Recombinant ZAK is produced via heterologous expression systems (e.g., E. coli, HEK293 cells) to study its structure-function relationships, enzymatic activity, and regulatory mechanisms. Its recombinant form allows precise investigation of substrate specificity, inhibitor screening, and structural studies using X-ray crystallography or cryo-EM. Researchers utilize it to explore ZAK’s involvement in diseases, including fibrosis, neurodegenerative disorders, and cancer, where dysregulated stress signaling contributes to pathogenesis. Additionally, it serves as a tool for developing targeted therapies, such as kinase inhibitors to modulate pathological stress responses. Current studies focus on its dual roles in pro-survival and pro-death signaling, highlighting context-dependent regulatory networks.
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