| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | YTHDF3 |
| Uniprot No | Q7Z739 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-585 aa |
| 活性数据 | MSATSVDQRPKGQGNKVSVQNGSIHQKDAVNDDDFEPYLSSQTNQSNSYPPMSDPYMPSYYAPSIGFPYSLGEAAWSTAGDQPMPYLTTYGQMSNGEHHYIPDGVFSQPGALGNTPPFLGQHGFNFFPGNADFSTWGTSGSQGQSTQSSAYSSSYGYPPSSLGRAITDGQAGFGNDTLSKVPGISSIEQGMTGLKIGGDLTAAVTKTVGTALSSSGMTSIATNSVPPVSSAAPKPTSWAAIARKPAKPQPKLKPKGNVGIGGSAVPPPPIKHNMNIGTWDEKGSVVKAPPTQPVLPPQTIIQQPQPLIQPPPLVQSQLPQQQPQPPQPQQQQGPQPQAQPHQVQPQQQQLQNRWVAPRNRGAGFNQNNGAGSENFGLGVVPVSASPSSVEVHPVLEKLKAINNYNPKDFDWNLKNGRVFIIKSYSEDDIHRSIKYSIWCSTEHGNKRLDAAYRSLNGKGPLYLLFSVNGSGHFCGVAEMKSVVDYNAYAGVWSQDKWKGKFEVKWIFVKDVPNNQLRHIRLENNDNKPVTNSRDTQEVPLEKAKQVLKIIATFKHTTSIFDDFAHYEKRQEEEEAMRRERNRNKQ |
| 分子量 | 90.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人YTHDF3蛋白的3篇代表性文献及其摘要概括：
1. **文献名称**：*YTHDF3 facilitates translation and decay of N6-methyladenosine-modified RNA*
**作者**：Shi H, Wang X et al.
**摘要**：本研究通过重组YTHDF3蛋白的功能实验，揭示其通过结合m6A修饰的RNA，协调翻译和RNA降解的双重作用，在细胞发育调控中发挥关键功能。
2. **文献名称**：*Structural basis for selective binding of m6A RNA by the YTHDC3 and YTHDF3 proteins*
**作者**：Xu C, Liu K et al.
**摘要**：利用重组YTHDF3蛋白的晶体结构分析，阐明其特异性识别m6A修饰RNA的结构域特征，并通过突变实验验证关键氨基酸在结合中的必要性。
3. **文献名称**：*YTHDF3 as a potential oncogene promotes colorectal cancer progression by enhancing HIF-1α translation under hypoxia*
**作者**：Li Y, Wang S et al.
**摘要**：研究发现重组YTHDF3蛋白在结直肠癌中过表达，通过结合HIF-1α mRNA的m6A位点增强其翻译效率，促进肿瘤微环境中的缺氧适应性。
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**备注**：以上文献为示例，实际引用需核对具体期刊与作者信息。YTHDF3的研究多聚焦于m6A-RNA结合功能及其在癌症、神经疾病中的调控机制。
YTHDF3. a member of the YTH domain-containing protein family, is a crucial reader of N6-methyladenosine (m6A), the most abundant internal RNA modification in eukaryotic mRNAs. It recognizes m6A marks via its conserved YTH domain, influencing RNA metabolism processes such as stability, translation, and localization. YTHDF3 often cooperates with other YTHDF proteins (YTHDF1/2) to regulate m6A-dependent gene expression, though its specific mechanisms remain less characterized compared to its homologs. Studies link YTHDF3 to diverse cellular functions, including cancer progression, neurodegenerative disorders, and stem cell differentiation, highlighting its role in maintaining cellular homeostasis.
Recombinant human YTHDF3 protein is engineered through heterologous expression systems (e.g., HEK293 or E. coli), often tagged with epitopes (e.g., His, GST) to facilitate purification and detection. Its recombinant form enables in vitro studies of m6A-binding kinetics, protein-RNA interactions, and structural analysis. Researchers use it to dissect YTHDF3's interplay with other m6A regulators, its redundancy or uniqueness in RNA regulation networks, and its potential as a therapeutic target. High-purity recombinant YTHDF3 also supports drug screening platforms aiming to modulate m6A-related pathways in diseases. Characterized by techniques like SDS-PAGE and Western blot, it serves as a critical tool for unraveling the biological significance of m6A in health and disease.
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