| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | YY1AP1 |
| Uniprot No | Q9H869 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-739 aa |
| 活性数据 | MGFSNMEDDGPEEEERVAEPQANFNTPQALRFEELLANLLNEQHQIAKELFEQLKMKKPSAKQQKEVEKVKPQCKEVHQTLILDPAQRKRLQQQMQQHVQLLTQIHLLATCNPNLNPEASSTRICLKELGTFAQSSIALHHQYNPKFQTLFQPCNLMGAMQLIEDFSTHVSIDCSPHKTVKKTANEFPCLPKQVAWILATSKVFMYPELLPVCSLKAKNPQDKILFTKAEDNLLALGLKHFEGTEFLNPLISKYLLTCKTARQLTVRIKNLNMNRAPDNIIKFYKKTKQLPVLGKCCEEIQPHQWKPPIEREEHRLPFWLKASLPSIQEELRHMADGAREVGNMTGTTEINSDQGLEKDNSELGSETRYPLLLPKGVVLKLKPVADRFPKKAWRQKRSSVLKPLLIQPSPSLQPSFNPGKTPAQSTHSEAPPSKMVLRIPHPIQPATVLQTVPGVPPLGVSGGESFESPAALPAMPPEARTSFPLSESQTLLSSALVPKVMMPSPASSMFRKPYVRRRPSKRRGARAFRCIKPAPVIHPASVIFTVPATTVKIVSLGGGCNMIQPVNAAVAQSPQTIPIATLLVNPTSFPCPLNQPLVASSVSPLIVSGNSVNLPIPSTPEDKAHMNVDIACAVADGENAFQGLEPKLEPQELSPLSATVFPKVEHSPGPPPVDKQCQEGLSENSAYRWTVVKTEEGRQALEPLPQGIQESLNNSSPGDLEEVVKMEPEDATEEISGFL |
| 分子量 | 107.03 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人YY1AP1蛋白的3条参考文献的模拟示例(注：以下内容为虚构，仅供格式参考)：
1. **《YY1AP1 promotes hepatocellular carcinoma progression through transcriptional activation of CDCA5》**
- **作者**：Li et al.
- **摘要**：研究发现重组YY1AP1蛋白通过结合YY1转录因子，调控下游靶基因CDCA5的表达，从而促进肝癌细胞增殖和转移。
2. **《Functional characterization of recombinant YY1AP1 in chromatin remodeling and DNA damage response》**
- **作者**：Zhang et al.
- **摘要**：利用大肠杆菌表达系统纯化重组YY1AP1蛋白，证实其通过参与染色质重塑复合体调控DNA损伤修复通路，影响基因组稳定性。
3. **《YY1AP1 interacts with the Hippo pathway effector YAP to drive oncogenic growth》**
- **作者**：Wang et al.
- **摘要**：研究发现重组YY1AP1蛋白与Hippo通路关键蛋白YAP形成复合物，共同激活促癌基因表达，促进肿瘤细胞侵袭和上皮间质转化(EMT)。
*注：以上文献信息为示例性描述，实际研究中需以真实出版物为准。若需具体文献，建议通过PubMed或Web of Science以“YY1AP1”或“recombinant YY1AP1”为关键词检索。*
YY1-associated protein 1 (YY1AP1) is a nuclear protein that functions as a transcriptional co-regulator, primarily interacting with the Yin Yang 1 (YY1) transcription factor to modulate gene expression. It plays a critical role in chromatin remodeling, DNA repair, and transcriptional regulation by facilitating the assembly of multiprotein complexes. Structurally, YY1AP1 contains a conserved N-terminal domain critical for binding YY1 and a C-terminal region involved in protein-protein interactions. Studies suggest its involvement in embryonic development, cell proliferation, and differentiation, with links to pathways like Wnt/β-catenin signaling.
Mutations or dysregulation of YY1AP1 are associated with human diseases, particularly Gabriele-de Vries syndrome, a rare neurodevelopmental disorder characterized by intellectual disability, facial dysmorphism, and growth abnormalities. Research highlights its role in maintaining genomic stability and regulating genes critical for neuronal function. Recombinant YY1AP1 protein, produced via bacterial or eukaryotic expression systems, is widely used to study molecular mechanisms, screen for binding partners, and explore therapeutic strategies. Its functional versatility and disease relevance make it a key target for understanding epigenetic regulation and developmental pathologies. Ongoing research aims to unravel its tissue-specific roles and potential as a biomarker or drug target.
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