| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | YTHDC1 |
| Uniprot No | Q96MU7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-709 aa |
| 活性数据 | MAADSREEKDGELNVLDDILTEVPEQDDELYNPESEQDKNEKKGSKRKSDRMESTDTKRQKPSVHSRQLVSKPLSSSVSNNKRIVSTKGKSATEYKNEEYQRSERNKRLDADRKIRLSSSASREPYKNQPEKTCVRKRDPERRAKSPTPDGSERIGLEVDRRASRSSQSSKEEVNSEEYGSDHETGSSGSSDEQGNNTENEEEGVEEDVEEDEEVEEDAEEDEEVDEDGEEEEEEEEEEEEEEEEEEEEYEQDERDQKEEGNDYDTRSEASDSGSESVSFTDGSVRSGSGTDGSDEKKKERKRARGISPIVFDRSGSSASESYADQTSKLKYVLQDARFFLIKSNNHENVSLAKAKGVWSTLPVNEKKLNLAFRSARSVILIFSVRESGKFQGFARLSSESHHGGSPIHWVLPAGMSAKMLGGVFKIDWICRRELPFTKSAHLTNPWNEHKPVKIGRDGQEIELECGTQLCLLFPPDESIDLYQVIHKMRHKRRMHSQPRSRGRPSRREPVRDVGRRRPEDYDIHNSRKKPRIDYPPEFHQRPGYLKDPRYQEVDRRFSGVRRDVFLNGSYNDYVREFHNMGPPPPWQGMPPYPGMEQPPHHPYYQHHAPPPQAHPPYSGHHPVPHEARYRDKRVHDYDMRVDDFLRRTQAVVSGRRSRPRERDRERERDRPRDNRRDRERDRGRDRERERERLCDRDRDRGERGRYRR |
| 分子量 | 109.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **文献名称**：*YTHDC1 mediates nuclear export of N6-methyladenosine methylated mRNAs*
**作者**：Roundtree IA, Luo GZ, Zhang Z, et al.
**摘要**：该研究揭示YTHDC1通过识别mRNA上的m6A修饰，调控甲基化mRNA的核输出。作者利用重组YTHDC1蛋白进行体外结合实验，证实其与mRNA结合依赖m6A修饰，并影响基因表达与细胞分化。
2. **文献名称**：*Structural basis for selective binding of m6A by YTHDC1*
**作者**：Xu C, Liu K, Ahmed H, et al.
**摘要**：本研究解析了重组人YTHDC1蛋白与m6A修饰RNA复合物的晶体结构，揭示了YTHDC1通过保守芳香残基选择性识别m6A的分子机制，为靶向YTHDC1的功能干预提供结构基础。
3. **文献名称**：*YTHDC1 regulates maternal mRNA stability in oocytes by m6A-dependent mechanisms*
**作者**：Kasowitz SD, Ma J, Anderson SJ, et al.
**摘要**：文章发现YTHDC1通过调控卵母细胞中母源mRNA的稳定性维持生殖发育。重组YTHDC1蛋白的体外实验表明其结合m6A修饰的RNA并延缓降解，暗示其在哺乳动物生殖中的关键作用。
4. **文献名称**：*The m6A reader YTHDC1 modulates viral RNA sensing and innate immunity*
**作者**：Kim GW, Imam H, Khan M, et al.
**摘要**：研究表明YTHDC1通过识别病毒RNA的m6A修饰调控宿主免疫应答。重组YTHDC1蛋白的互作实验证实其抑制RIG-I信号通路，揭示了其在抗病毒反应中的双重功能。
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以上文献均涉及重组YTHDC1蛋白的应用，涵盖功能机制、结构解析及病理生理研究，可为进一步实验提供参考。
YTHDC1 (YTH Domain-Containing Protein 1) is an RNA-binding protein that plays a critical role in regulating post-transcriptional gene expression by recognizing N6-methyladenosine (m6A), the most abundant internal modification in eukaryotic mRNA. It belongs to the YTH protein family, characterized by a conserved YTH domain that selectively binds m6A-modified RNAs. Structurally, YTHDC1 contains an N-terminal arginine/glycine-rich region involved in protein-protein interactions and nuclear localization, and a C-terminal YTH domain responsible for m6A recognition.
Primarily localized in the nucleus, YTHDC1 regulates RNA splicing, export, and stability by interacting with spliceosome components (e.g., SRSF3) and nuclear pore complexes. It facilitates m6A-dependent mRNA export to the cytoplasm, influences alternative splicing by recruiting splicing factors, and maintains genomic stability through DNA repair mechanisms. Dysregulation of YTHDC1 is implicated in diseases such as cancer, where it may act as a tumor suppressor or promoter depending on context, and neurological disorders linked to aberrant RNA processing.
Recombinant YTHDC1 protein, produced via bacterial or mammalian expression systems, is widely used to study m6A-related molecular mechanisms, screen for small-molecule modulators, and explore therapeutic strategies targeting epitranscriptomic pathways. Its role in bridging m6A methylation with cellular function makes it a focal point in understanding RNA epigenetics and disease biology.
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