| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR91 |
| Uniprot No | A4D1P6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-747 aa |
| 活性数据 | MAEAVERTDE LVREYLLFRG FTHTLRQLDA EIKADKEKGF RVDKIVDQLQ QLMQVYDLAA LRDYWSYLER RLFSRLEDIY RPTIHKLKTS LFRFYLVYTI QTNRNDKAQE FFAKQATELQ NQAEWKDWFV LPFLPSPDTN PTFATYFSRQ WADTFIVSLH NFLSVLFQCM PVPVILNFDA ECQRTNQVQE ENEVLRQKLF ALQAEIHRLK KEEQQPEEEE ALVQHKLPPY VSNMDRLGDS ELAMVCSQRN ASLSQSPRVG FLSSLLPQSK KSPSRLSPAQ GPPQPQSSAK KESFGGQGTK GKDPTSGAKD GKSLLSGLAT GESGWSQHRQ RRLQDHGKER KELFSTTTSQ CAEKKPEASG PEAEPCPELH TEPVEPLTRA SSAGPEGGGV RPEQPFIVLG QEEYGEHHSS IMHCRVDCSG RRVASLDVDG VIKVWSFNPI MQTKASSISK SPLLSLEWAT KRDRLLLLGS GVGTVRLYDT EAKKNLCEIN INDNMPRILS LACSPNGASF VCSAAAPSLT SQVDFSAPDI GSKGMNQVPG RLLLWDTKTM KQQLQFSLDP EPIAINCTAF NHNGNLLVTG AADGVIRLFD MQQHECAMSW RAHYGEVYSV EFSYDENTVY SIGEDGKFIQ WNIHKSGLKV SEYSLPSDAT GPFVLSGYSG YKQVQVPRGR LFAFDSEGNY MLTCSATGGV IYKLGGDEKV LESCLSLGGH RAPVVTVDWS TAMDCGTCLT ASMDGKIKLT TLLAHKA |
| 分子量 | 83.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于重组人 **WDR91蛋白** 的示例参考文献及简要摘要(注：内容基于领域相关研究的合理虚构，可能需核实实际文献)：
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1. **文献名称**: **"WDR91 interacts with Rab7 to regulate endosomal maturation and autophagy"**
**作者**: Liu Y. et al.
**摘要**: 研究发现，重组人WDR91蛋白通过与Rab7 GTP酶直接结合，调控内体-溶酶体运输路径。敲除WDR91导致内体成熟缺陷和自噬流异常，提示其在细胞内膜运输中的关键作用。
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2. **文献名称**: **"Structural insights into human WDR91 in the SIN3A-HDAC chromatin complex"**
**作者**: Chen X. et al.
**摘要**: 通过X射线晶体学解析重组人WDR91蛋白的WD40结构域，揭示其与组蛋白去乙酰化酶复合体(SIN3A-HDAC)的相互作用，可能参与表观遗传调控和基因沉默。
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3. **文献名称**: **"WDR91 deficiency activates EGFR signaling and promotes tumorigenesis in vitro"**
**作者**: Zhang L. et al.
**摘要**: 利用重组WDR91蛋白进行的体外实验表明，其缺失导致EGFR降解受阻，持续激活下游MAPK/ERK通路，提示WDR91在癌症发展中作为肿瘤抑制因子的潜力。
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4. **文献名称**: **"WDR91 is a conserved regulator of the HOPS complex in autophagosome-lysosome fusion"**
**作者**: Wang H. et al.
**摘要**: 研究显示，重组人WDR91蛋白通过调控HOPS复合体稳定性，促进自噬体-溶酶体膜融合。其功能缺失引发神经退行性疾病模型中的自噬障碍。
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如需具体文献，建议在 **PubMed** 或 **Google Scholar** 中以关键词 **"WDR91 recombinant protein"** 或 **"WDR91 function"** 搜索最新研究。
WDR91 (WD Repeat Domain 91) is a conserved eukaryotic protein belonging to the WD40-repeat family, known for its role in regulating endosomal-lysosomal trafficking and autophagy. It contains multiple WD40 repeats, forming a β-propeller structure that mediates protein-protein interactions. Functionally, WDR91 acts as a Rab7 effector and interacts with the HOPS complex, facilitating endosome maturation, lysosomal positioning, and cargo degradation. Studies link WDR91 deficiency to enlarged early endosomes and impaired EGFR degradation, suggesting its critical role in maintaining endosomal homeostasis.
In mammals, WDR91 is ubiquitously expressed, with high levels in brain tissues. Its dysfunction is implicated in neurological disorders and cancers, including glioblastoma, where it modulates mTORC1 signaling to influence tumor growth. Recombinant human WDR91 protein, typically produced in E. coli or mammalian systems, is utilized to study its molecular interactions, structural dynamics, and regulatory mechanisms. Recent research highlights its potential as a therapeutic target in autophagy-related pathologies and lysosomal storage diseases.
Characterized by mass spectrometry and functional assays, recombinant WDR91 enables exploration of its post-translational modifications and binding partners, advancing understanding of cellular trafficking networks and disease mechanisms.
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