| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR85 |
| Uniprot No | Q9BTV6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-452 aa |
| 活性数据 | MMGCFALQTV DTELTADSVE WCPLQGCRHL LACGTYQLRR PEDRPAGPQN KGGMEVKEPQ VRLGRLFLYS FNDNNSIHPL VEVQRKDTSA ILDMKWCHIP VAGHALLGLA DASGSIQLLR LVESEKSHVL EPLSSLALEE QCLALSLDWS TGKTGRAGDQ PLKIISSDST GQLHLLMVNE TRPRLQKVAS WQAHQFEAWI AAFNYWHPEI VYSGGDDGLL RGWDTRVPGK FLFTSKRHTM GVCSIQSSPH REHILATGSY DEHILLWDTR NMKQPLADTP VQGGVWRIKW HPFHHHLLLA ACMHSGFKIL NCQKAMEERQ EATVLTSHTL PDSLVYGADW SWLLFRSLQR APSWSFPSNL GTKTADLKGA SELPTPCHEC REDNDGEGHA RPQSGMKPLT EGMRKNGTWL QATAATTRDC GVNPEEADSA FSLLATCSFY DHALHLWEWE GN |
| 分子量 | 50.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WDR85蛋白的参考文献示例(基于现有研究领域推断，部分内容为假设性概括)：
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1. **标题**：*WDR85 interacts with the CRL4-DCAF12 ubiquitin ligase and regulates ribosome biogenesis*
**作者**：Smith A, et al.
**摘要**：研究揭示了WDR85作为CRL4-DCAF12泛素连接酶复合物的互作蛋白，通过调控核糖体RNA加工影响细胞增殖。重组人WDR85蛋白的体外实验证实其直接结合DCAF12.并参与rRNA前体修饰的分子机制。
2. **标题**：*Structural and functional analysis of recombinant human WDR85 in chromatin remodeling*
**作者**：Zhang L, et al.
**摘要**：通过大肠杆菌表达系统获得高纯度重组WDR85蛋白，晶体结构显示其WD40结构域形成典型β-螺旋桨构象。功能实验表明WDR85通过招募组蛋白去乙酰化酶复合物(HDAC)调控染色质重塑和基因沉默。
3. **标题**：*Dysregulation of WDR85 in hepatocellular carcinoma and its role in tumorigenesis*
**作者**：Tanaka K, et al.
**摘要**：研究发现肝癌组织中WDR85表达显著下调，过表达重组WDR85蛋白可抑制肿瘤细胞生长并诱导凋亡，提示其作为肿瘤抑制因子的潜在功能。
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**说明**：以上文献为模拟示例，具体研究请通过学术数据库(如PubMed、Web of Science)以关键词“WDR85”或“recombinant WDR85”检索最新论文。真实文献可能存在更详细的功能研究(如调控RNA代谢、干细胞分化等方向)。
WDR85 (WD Repeat Domain 85) is a human protein belonging to the WD40 repeat family, characterized by conserved tandem repeats of ~40 amino acids that form β-propeller structures. These proteins typically act as scaffolds for molecular complexes, facilitating protein-protein interactions. WDR85 is encoded by the *WDR85* gene located on chromosome 9 (9q34.3) and is ubiquitously expressed across tissues, with higher levels in the brain, testes, and immune cells. Functionally, it plays roles in RNA processing, epigenetic regulation, and chromatin remodeling by interacting with the m6A mRNA methylation complex (METTL3/METTL14) and histone modification machinery. Studies suggest its involvement in cellular differentiation, genome stability, and neurodevelopment. Dysregulation of WDR85 has been loosely associated with neurological disorders and cancers. Recombinant human WDR85 protein, produced via heterologous expression systems like *E. coli* or mammalian cells, retains structural integrity and biological activity. It serves as a vital tool for studying RNA epigenetics, protein interaction networks, and mechanisms underlying transcriptional regulation. Its recombinant form enables *in vitro* binding assays, structural studies (e.g., X-ray crystallography), and screens for therapeutic agents targeting epigenetic pathways. Research on WDR85 continues to expand its role in linking RNA modifications to chromatin dynamics and disease pathways.
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