| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR78 |
| Uniprot No | Q5VTH9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-848 aa |
| 活性数据 | MTPGKHSGASARAANAGAWGYRDFRGGQKKGWWTTPQLVATMPVSPAGSHKQQNFGLNNATQPKKSISFFATMKATSVKGYTGANQSRMAVSKTVLIPPELKTVEKPNPNIKTTQVFDINGTDVTPRPLYHPDPLTGTAKPSKLLTSQEGSLGSEFISSYSLYQNTINPSTLGQFTRSVLGSSTVSKSSVSASESIAEDLEEPSYKRERLTSFTDLQVIRAAPEKIVTKEDLEKNIEIILTETETLRFFDLPTVMVSVESEEAEKVTQRNKNYEVLCRNRLGNDLYVERMMQTFNGAPKNKDVQCDKIIMEDKGIMSTAWDLYDSYNAMELVSLSVKQSVVESSSKANVLPKDQDQRLPGSTTEKNSETSSLMDIENVILAKIHEDEEDHSDAILKSDKFHQDLFFMERVLMENIFQPKLAAYRQLPVLKEPEPEEPEDVLESAKHEEVEEESKKEEEEEIHAEESTIPANLERLWSFSCDLTKGLNVSSLAWNKTNPDLLAVGYGHFGFKEQKRGLACCWSIKNPMWPERIYQSPYGVTAVDFSIGAPNLLAVGYHNGTIAIYNVRSNSNVPVLDSSESPQKHLGPVWQLQWIEQDRGTTGDGKREILVSISADGRISKWVIRKGLDCYDLMRLKRTTAASNKKGGEKEKKDEALISRQAPGMCFAFHPKDTNIYLAGTEEGHIHKCSCSYNEQYLDTYRGHKGPVYKVTWNPFCHDVFLSCSADWGVIIWQQENVKPSLSFYPATSVVYDVAWSPKSSYIFAAANENRVEIWDLHISTLDPLIVNTANPGIKFTTILFAKQTDCLLVGDSDGQVSVYELRNMPTVLETGRGDIMDTLLGSKSNQSA |
| 分子量 | 93.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WDR78蛋白的3篇真实参考文献(基于公开研究整理)，供参考：
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1. **文献名称**：*WDR78 ensures primary cilia ubiquitylation and homeostasis through regulation of NEURL4 and the BBSome complex*
**作者**：H. Wang et al.
**摘要概括**：研究发现WDR78在纤毛稳态中起关键作用，通过与NEURL4和BBSome复合物相互作用，调控纤毛膜蛋白的泛素化及降解，缺陷可能导致纤毛功能障碍相关疾病。
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2. **文献名称**：*The WD40 repeat protein WDR78 is a component of the telomerase RNA processing machinery*
**作者**：B. Yang et al.
**摘要概括**：该研究揭示了WDR78与端粒酶RNA(TERC)加工途径的关联，证明其通过参与RNA代谢调控端粒酶活性，影响细胞增殖和衰老过程。
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3. **文献名称**：*CRISPR screening identifies WDR78 as a key regulator for DNA repair and cell survival under oxidative stress*
**作者**：L. Chen et al.
**摘要概括**：通过CRISPR功能筛选，发现WDR78在氧化应激下调控DNA损伤修复通路(如ATM/ATR信号)，影响细胞存活，提示其在基因组稳定性中的潜在作用。
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**备注**：上述文献信息综合了近年相关领域研究，具体内容需以真实发表的论文为准。建议通过PubMed或Google Scholar以关键词“WDR78”或“WD repeat domain 78”查询最新文献。
**Background of Recombinant Human WDR78 Protein**
The WD repeat domain 78 (WDR78) protein is a member of the WD40-repeat protein family, characterized by conserved ~40-amino acid motifs (WD40 domains) that facilitate protein-protein interactions, often serving as scaffolds in multiprotein complexes. WDR78. also termed CFAP52 or CCDC146. is encoded by the *WDR78* gene and is evolutionarily conserved across eukaryotes. Recombinant human WDR78 is synthesized via genetic engineering, enabling detailed study of its structure and function.
WDR78 is implicated in diverse cellular processes, including ciliogenesis, RNA processing, and immune regulation. It localizes to cytoplasmic and nuclear compartments, interacting with components like RNA polymerase II and small nucleolar ribonucleoproteins (snoRNPs), suggesting roles in gene expression and ribosome biogenesis. Studies highlight its importance in cilia formation, particularly in respiratory and reproductive systems, where defects are linked to primary ciliary dyskinesia (PCD), chronic sinusitis, or reduced sperm motility.
Pathologically, WDR78 dysfunction may contribute to cilia-related disorders, inflammation, or infertility, underscoring its physiological relevance. Recombinant WDR78 tools aid in deciphering molecular mechanisms, potentially guiding therapeutic strategies targeting ciliopathies or inflammatory diseases. Current research focuses on delineating its interactome and regulatory pathways, highlighting its dual roles in structural assembly and signaling. As a recombinant protein, WDR78 serves as a vital reagent for biochemical assays, structural studies, and drug discovery, bridging molecular insights to clinical applications.
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