| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WDR76 |
| Uniprot No | Q9H967 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-626 aa |
| 活性数据 | MSRSGAAAEK ADSRQRPQMK VNEYKENQNI AYVSLRPAQT TVLIKTAKVY LAPFSLSNYQ LDQLMCPKSL SEKNSNNEVA CKKTKIKKTC RRIIPPKMKN TSSKAESTLQ NSSSAVHTES NKLQPKRTAD AMNLSVDVES SQDGDSDEDT TPSLDFSGLS PYERKRLKNI SENADFFASL QLSESAARLR EMIEKRQPPK SKRKKPKREN GIGCRRSMRL LKVDPSGVSL PAAPTPPTLV ADETPLLPPG PLEMTSENQE DNNERFKGFL HTWAGMSKPS SKNTEKGLSS IKSYKANLNG MVISEDTVYK VTTGPIFSMA LHPSETRTLV AVGAKFGQVG LCDLTQQPKE DGVYVFHPHS QPVSCLYFSP ANPAHILSLS YDGTLRCGDF SRAIFEEVYR NERSSFSSFD FLAEDASTLI VGHWDGNMSL VDRRTPGTSY EKLTSSSMGK IRTVHVHPVH RQYFITAGLR DTHIYDARRL NSRRSQPLIS LTEHTKSIAS AYFSPLTGNR VVTTCADCNL RIFDSSCISS KIPLLTTIRH NTFTGRWLTR FQAMWDPKQE DCVIVGSMAH PRRVEIFHET GKRVHSFGGE YLVSVCSINA MHPTRYILAG GNSSGKIHVF MNEKSC |
| 分子量 | 69.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人WDR76蛋白的模拟参考文献示例(请注意，这些文献为虚构示例，实际引用需核实)：
1. **《WDR76 interacts with CUL4-DDB1 to regulate histone H2A ubiquitination》**
*Chen et al., 2018*
摘要：本研究揭示WDR76作为CRL4泛素连接酶复合物的底物识别组分，参与组蛋白H2A的泛素化修饰，调控染色质动态和DNA修复过程。
2. **《WDR76 maintains genomic stability through ATM/ATR signaling pathway》**
*Liu et al., 2017*
摘要：发现WDR76在DNA损伤应答中关键作用，通过激活ATM/ATR信号通路促进细胞周期检查点调控，缺失WDR76导致基因组不稳定性增加。
3. **《Tumor suppressor role of WDR76 in hepatocellular carcinoma via p53-mediated apoptosis》**
*Li et al., 2019*
摘要：揭示WDR76在肝癌中通过稳定p53蛋白诱导细胞凋亡，其低表达与患者预后不良相关，提示其抑癌功能。
4. **《Optimizing recombinant human WDR76 expression in E. coli for structural studies》**
*Saito et al., 2020*
摘要：报道在大肠杆菌中高效表达和纯化重组人WDR76蛋白，优化条件获得可溶性蛋白，用于晶体结构和互作网络分析。
*注：以上内容为模拟生成，实际文献需通过学术数据库检索确认。*
WDR76 (WD repeat-containing protein 76) is a conserved eukaryotic protein belonging to the WD40 repeat family, known for its scaffold-like structure facilitating protein-protein interactions. Characterized by multiple tandem WD40 repeats forming a β-propeller fold, it plays versatile roles in cellular processes. Studies link WDR76 to genomic stability maintenance, DNA damage repair, and epigenetic regulation. It interacts with the CRL4 ubiquitin ligase complex to mediate proteasomal degradation of specific substrates, including proteins involved in cell cycle control and chromatin remodeling. WDR76 also participates in transcriptional regulation, potentially influencing histone modifications and heterochromatin formation.
In mammalian systems, WDR76 is implicated in spermatogenesis, telomere homeostasis, and tumor suppression. Its nuclear-cytoplasmic shuttling suggests context-dependent functions. Dysregulation of WDR76 has been associated with carcinogenesis, where it may act as an oncogene or tumor suppressor depending on cellular conditions. For instance, WDR76 depletion promotes genomic instability, while its overexpression correlates with poor prognosis in certain cancers. Current research focuses on elucidating its substrate specificity, post-translational modifications, and tissue-specific roles. Recombinant human WDR76 proteins are widely used to study its molecular mechanisms, offering potential therapeutic targets for cancer and DNA repair-related disorders.
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