| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WBP5 |
| Uniprot No | Q9UHQ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-104 aa |
| 活性数据 | MKSCQKMEGK PENESEPKHE EEPKPEEKPE EEEKLEEEAK AKGTFRERLI QSLQEFKEDI HNRHLSNEDM FREVDEIDEI RRVRNKLIVM RWKVNRNHPY PYLM |
| 分子量 | 12.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于假设的示例性参考文献，供参考(请注意，以下内容为虚构，实际文献需通过学术数据库查询)：
1. **文献名称**: "Efficient Expression and Purification of Recombinant Human WBP5 in E. coli"
**作者**: Smith J, et al.
**摘要**: 本研究开发了一种优化的大肠杆菌表达系统，用于高效生产重组人WBP5蛋白。通过密码子优化和亲和层析纯化，获得高纯度蛋白，为后续结构功能研究奠定基础。
2. **文献名称**: "Ubiquitin Ligase Activity of WBP5 and Its Role in TGF-β Signaling"
**作者**: Chen L, et al.
**摘要**: 文中验证了重组人WBP5的E3泛素连接酶活性，并发现其通过WW结构域与Smad4相互作用，调控TGF-β信号通路的泛素化降解过程，影响细胞分化。
3. **文献名称**: "Recombinant WBP5 Suppresses Tumor Growth via Apoptosis Induction in Colorectal Cancer Models"
**作者**: Wang Y, et al.
**摘要**: 通过体外实验表明，重组WBP5蛋白能激活caspase通路，诱导结直肠癌细胞凋亡，并在小鼠模型中显著抑制肿瘤生长，提示其潜在抗癌应用。
**建议**：实际研究中，请通过PubMed、Google Scholar等平台，以“WWP5”(或确认正确名称)、“recombinant protein”、“ubiquitin ligase”等关键词检索最新文献。若WBP5存在拼写变体，需结合基因编号(如HGNC)进一步确认。
**Background of Recombinant Human WBP5 Protein**
The WW domain-binding protein 5 (WBP5), also known as WBP11 or NAGBP1. is a multifunctional adaptor protein involved in cellular processes such as transcriptional regulation, RNA splicing, and protein-protein interactions. It belongs to the WW domain-binding protein family, characterized by conserved PPXY motifs that mediate interactions with WW domain-containing partners, including Nedd4-family E3 ubiquitin ligases. WBP5 plays roles in ubiquitination pathways, mRNA processing, and signal transduction, impacting cell proliferation, differentiation, and apoptosis.
Recombinant human WBP5 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, functional protein for research. Its recombinant form retains critical domains, such as the PPXY motifs and regions for RNA binding, enabling studies on its molecular interactions and regulatory mechanisms. Researchers utilize recombinant WBP5 to explore its involvement in diseases like cancer, neurodevelopmental disorders, and viral infections, where dysregulated ubiquitination or splicing pathways are implicated.
Applications include *in vitro* binding assays, structural studies, and functional analyses to decipher its role in cellular homeostasis. By enabling precise manipulation, recombinant WBP5 aids in developing targeted therapies modulating ubiquitin-dependent degradation or RNA metabolism.
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